荧光原位杂交技术分析人结肠菌群方法研究

建立荧光原位杂交技术分析人体内结肠菌群的方法。取受试者新鲜粪便 ,选用 5种特异性的 16SrRNA寡核苷酸探针 ,检测粪便样本收集后的保存时间、温度 ,离心条件及样本固定液存放时间对杂交计数结果的影响。结果建立最佳实验条件为 :粪便样本收集后应尽快在 4℃下保存 ,放置时间不要超过 12小时即作处理 ;样本的适宜离心条件为 70 0g 2分钟 ;样本用多聚甲醛固定后在 - 80℃下存放时间不要超过 5个月。该方法具有较好的稳定性 ,可以有效地检出个体之间结肠菌群的差异。     

Simultaneous changes of leg circumference and interface pressure under different compression bandages.

OBJECTIVES: To assess the validity of measuring changes of sub bandage pressure and leg circumference in different body positions for an in vivo characterization of the elastic properties of bandage systems. DESIGN: Experimental study. MATERIALS AND METHODS: Different compression bandages were applied on the leg. The variations of interface pressure and leg circumference above the inner ankle (that depends on the elastic property of the bandage) were measured simultaneously by a pressure transducer and by strain-gauge plethysmography in 50 patients. Stiffness is defined as the increase of pressure per increase of circumference. RESULTS: The most consistent parameter to differentiate elastic from inelastic bandages was the pressure-difference between the standing and the lying position corrected for the actual increase of leg circumference (modified static stiffness index, mSSI; sensitivity and specificity 100%). Neglecting the individual changes of the circumference and considering the pressure difference alone allows a differentiation which is slightly less accurate (sensitivity 100%, specificity 88%) but much simpler to use. CONCLUSIONS: The static stiffness index is a useful tool to differentiate elastic from inelastic bandage material even without correction for the individual increase of leg circumference.