全文获取类型
收费全文 | 68268篇 |
免费 | 7909篇 |
国内免费 | 4808篇 |
专业分类
耳鼻咽喉 | 675篇 |
儿科学 | 760篇 |
妇产科学 | 548篇 |
基础医学 | 6757篇 |
口腔科学 | 1658篇 |
临床医学 | 8895篇 |
内科学 | 8214篇 |
皮肤病学 | 706篇 |
神经病学 | 2922篇 |
特种医学 | 2665篇 |
外国民族医学 | 31篇 |
外科学 | 7090篇 |
综合类 | 14744篇 |
现状与发展 | 21篇 |
一般理论 | 6篇 |
预防医学 | 5848篇 |
眼科学 | 1630篇 |
药学 | 7615篇 |
117篇 | |
中国医学 | 5261篇 |
肿瘤学 | 4822篇 |
出版年
2024年 | 322篇 |
2023年 | 1180篇 |
2022年 | 3191篇 |
2021年 | 4011篇 |
2020年 | 3310篇 |
2019年 | 2387篇 |
2018年 | 2421篇 |
2017年 | 2600篇 |
2016年 | 2302篇 |
2015年 | 3578篇 |
2014年 | 4379篇 |
2013年 | 4323篇 |
2012年 | 6391篇 |
2011年 | 6686篇 |
2010年 | 4948篇 |
2009年 | 4080篇 |
2008年 | 4564篇 |
2007年 | 4047篇 |
2006年 | 3601篇 |
2005年 | 2984篇 |
2004年 | 1952篇 |
2003年 | 1617篇 |
2002年 | 1235篇 |
2001年 | 958篇 |
2000年 | 899篇 |
1999年 | 694篇 |
1998年 | 390篇 |
1997年 | 356篇 |
1996年 | 296篇 |
1995年 | 241篇 |
1994年 | 198篇 |
1993年 | 139篇 |
1992年 | 124篇 |
1991年 | 105篇 |
1990年 | 112篇 |
1989年 | 78篇 |
1988年 | 65篇 |
1987年 | 56篇 |
1986年 | 45篇 |
1985年 | 36篇 |
1984年 | 13篇 |
1983年 | 10篇 |
1982年 | 10篇 |
1981年 | 9篇 |
1980年 | 4篇 |
1979年 | 10篇 |
1978年 | 4篇 |
1976年 | 4篇 |
1975年 | 3篇 |
1974年 | 3篇 |
排序方式: 共有10000条查询结果,搜索用时 0 毫秒
1.
2.
Near-infrared diffuse correlation spectroscopy/tomography (DCS/DCT) has recently emerged as a noninvasive measurement/imaging technology for tissue blood flow. In DCT studies, the high-dense collection of light temporal autocorrelation curves (g2(τ)) via fiber array are critical for image reconstruction of blood flow. Previously, the camera-based fiber array limits the field of view (FOV), precluding its applications on large-size human tissues. The line-shape fiber probe based on lens combination, which is predominantly used in current DCT studies, requires rotated-scanning over the surface of target tissue, substantially prolonging the measurement time and increasing the system instability. In this study, we design a noncontact optical probe for DCT based on collimating micro-lens fiber array, termed as FA-nc-DCT system. For each source/detector fiber, a single optical path was collimated by coupling with one micro-lens in the fiber array that is integrated in a square-shape base. Additionally, an 8×8 optical switch is used to share the hardware laser and detectors without spatial scanning. The FA-nc approach for the precise collection of g2(τ) curves was validated through a speed-varied phantom experiment and the human experiments of cuff occlusion, from which the expected value of the blood flow index (BFI) was obtained. Furthermore, the flow anomaly in the phantom and the ischemic muscle in human were accurately reconstructed from the FA-nc-DCT system, which is combined with the imaging framework based on the Nth-order linear algorithm that we recently created. Those outcomes demonstrated the great potential of FA-nc-DCT technology for fast and robust imaging of various diseases such as human breast cancers. 相似文献
3.
Bortezomib is a novel proteasome inhibitor, which has been successfully used to treat mantle cell lymphoma and multiple myeloma. However, the direct effects of bortezomib on acute promyelocytic leukaemia (APL) have not been fully investigated. In the present study, the WST-8 assay, western blotting, flow cytometry, monodansylcadaverine staining and transmission electron microscopy were performed. It was demonstrated that bortezomib treatment induced a time- and dose-dependent decrease in the viability of NB4 cells. Bortezomib treatment induced cell apoptosis in NB4 cells, as assessed by Annexin V/propidium iodide analysis, and the detection of cleaved caspase-3, cleaved poly(ADP-ribose) polymerase, Bax and Bcl-2 expression. Furthermore, bortezomib treatment induced autophagy in NB4 cells, as indicated by autophagosome formation, p62 degradation, LC3-I to LC3-II conversion and formation of acidic autophagic vacuoles. Notably, autophagy induced by bortezomib was initiated prior to apoptosis. Inhibition of autophagy by knocking down Beclin-1 expression increased bortezomib-induced apoptosis in NB4 cells. Therefore, the present study revealed that the combination of bortezomib and autophagy inhibition may be a potential treatment strategy for APL. 相似文献
5.
Lei Yang You-Ling Shi Yan Ma Wei-Wei Ren Guang-Ming Pang Jiao Liu 《APMIS : acta pathologica, microbiologica, et immunologica Scandinavica》2022,130(1):43-52
Krüppel-like factor 16 (KLF16), a member of the Krüppel-like factor (KLF) family, has been extensively investigated in multiple cancer types. However, the role of KLF16 in oral squamous cell carcinoma (OSCC) remains unknown. Thus, we conducted this study to investigate its related mechanism. KLF16 expression in OSCC cell lines was quantified by western blotting. Then, OECM1 and OC3 cells were divided into Blank, siCtrl, siKLF16#1 and siKLF16#2 groups. Subsequently, cell proliferation was detected using 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide (MTT) assays, cell migration and invasion were detected with wound healing and Transwell assays, and cell cycle distribution and cell apoptosis were detected via flow cytometry. KLF16, p21, CDK4, Cyclin D1 and p-Rb expression was detected by western blotting. Finally, xenograft models were established in nude mice to observe the in vivo effects of KLF16 on OSCC. KLF16 protein expression was upregulated in OSCC cells. Compared to the cells in the Blank group, the OECM1 and OC3 cells in the siKLF16#1 group and siKLF16#2 group exhibited a sharp decrease in proliferation but a remarkable increase in apoptosis. Moreover, the proportion of cells in the G0/G1 phase notably increased and that in the S phase decreased, with evident decreases in cell invasion and migration. Moreover, KLF16, cyclin-dependent kinase 4 (CDK4), Cyclin D1 and p-Rb protein expression was upregulated, but p21 expression was downregulated. The mice in the siKLF16#1 and siKLF16#2 xenograft model groups exhibited slower tumour growth and smaller tumours with evident downregulation of Ki67 expression compared to the mice in the Blank group. KLF16 expression was upregulated in OSCC cells, and interfering with KLF16 led to cell cycle arrest, inhibited OSCC cell growth and promoted cell apoptosis. 相似文献
6.
7.
8.
9.
10.
周蕾 《国际妇产科学杂志》2015,42(1):91-95
宫颈癌对妇女健康构成严重威胁,人乳头瘤病毒感染与宫颈病变及宫颈癌的发生密切相关。关于宫颈癌发生发展的机制仍在研究中。近年研究发现一种多功能核蛋白,即死亡结构域相关蛋白(death domain associated protein,Daxx),其与细胞内蛋白或病毒蛋白相互作用,参与调节细胞凋亡、转录调控、抗病毒等细胞活动,在不同途径中发挥不同的生理或病理作用。通过对Daxx功能及其作用机制的研究有助于进一步阐明宫颈癌发生发展的机制,有助于发现新的预防和治疗方法。综述Daxx的一般特性和研究现况及其在宫颈病变的研究进展。 相似文献