全文获取类型
收费全文 | 796篇 |
免费 | 69篇 |
国内免费 | 4篇 |
专业分类
耳鼻咽喉 | 6篇 |
儿科学 | 46篇 |
妇产科学 | 13篇 |
基础医学 | 118篇 |
口腔科学 | 26篇 |
临床医学 | 55篇 |
内科学 | 216篇 |
皮肤病学 | 22篇 |
神经病学 | 27篇 |
特种医学 | 23篇 |
外科学 | 84篇 |
综合类 | 13篇 |
一般理论 | 2篇 |
预防医学 | 41篇 |
眼科学 | 14篇 |
药学 | 101篇 |
中国医学 | 11篇 |
肿瘤学 | 51篇 |
出版年
2023年 | 5篇 |
2022年 | 9篇 |
2021年 | 24篇 |
2020年 | 16篇 |
2019年 | 21篇 |
2018年 | 32篇 |
2017年 | 21篇 |
2016年 | 19篇 |
2015年 | 20篇 |
2014年 | 36篇 |
2013年 | 37篇 |
2012年 | 68篇 |
2011年 | 44篇 |
2010年 | 36篇 |
2009年 | 28篇 |
2008年 | 47篇 |
2007年 | 49篇 |
2006年 | 44篇 |
2005年 | 39篇 |
2004年 | 63篇 |
2003年 | 49篇 |
2002年 | 22篇 |
2001年 | 17篇 |
2000年 | 18篇 |
1999年 | 10篇 |
1998年 | 8篇 |
1996年 | 2篇 |
1995年 | 3篇 |
1993年 | 2篇 |
1992年 | 7篇 |
1991年 | 9篇 |
1990年 | 8篇 |
1989年 | 5篇 |
1988年 | 4篇 |
1987年 | 5篇 |
1985年 | 3篇 |
1984年 | 3篇 |
1981年 | 3篇 |
1980年 | 2篇 |
1979年 | 5篇 |
1978年 | 2篇 |
1977年 | 2篇 |
1975年 | 3篇 |
1969年 | 1篇 |
1968年 | 2篇 |
1967年 | 1篇 |
1966年 | 2篇 |
1965年 | 2篇 |
1964年 | 1篇 |
1962年 | 1篇 |
排序方式: 共有869条查询结果,搜索用时 15 毫秒
1.
Aristolochic acid, an alkaloid from the plant Aristolochia species, interacts with the major basic phospholipase A2 from Vipera russelli venom. It is an uncompetitive inhibitor with a Ki of 9.9 X 10(-4)M when phosphatidylcholine is used as substrate. The inhibition of direct and indirect hemolysis is higher compared to the inhibition of phosphatidylcholine hydrolysis. Edema-inducing activity of Vipera russelli phospholipase A2 is inhibited by aristolochic acid when injected either as a mixture or separately. Both i.m. and i.p. administration of aristolochic acid following phospholipase injection are equally effective in inhibiting edema. The alkaloid inhibits the edema-inducing activity as soon as it reaches the site, but does not aid in recovery. Aristolochic acid failed to inhibit other pathological activities of the enzyme. 相似文献
2.
3.
Chetan Anand Akmal Pasha M. D. Swetha Putte Gowda B. E. Amitha Rani N. G. K. Karanth 《Food and Agricultural Immunology》2007,18(1):39-51
A polyclonal antibody-based enzyme-linked immunosorbent assay (ELISA) method was developed for the N-methylcarbamate insecticide bendiocarb (2,2-dimethyl-1,3-benzodioxol-4-yl methylcarbamate). Two novel haptens having dimethylbenzodioxyl and dimethylbenzofuranyl groups connected to oxyacetyl-γ-aminobutanoic acid and oxyacetyl-β-alanine spacer arm respectively were synthesised. The first hapten was conjugated to carrier proteins to make antigens that were used to raise polyclonal antibodies in rabbits. The antibodies specifically recognised bendiocarb and its metabolite 2,2-dimethyl-1,3-benzodiox-4-ol with an IC50 value of 9 ppb (ng ml-1). The assay was standardised using the competitive ELISA format at 0.0625 µg antibody concentration and at 1/10k pesticide-HRP dilution. Matrix effect studies were carried out in four vegetable and cereal food samples. Matrix effect elimination in cabbage, cauliflower and rice was achieved by simple dilution of the extract. Five different approaches were attempted to achieve matrix clean up in paddy rice. C-18 column and gel permeation column chromatography (GPC) helped in the matrix removal. The spike and recovery studies for all the four food samples gave a recovery in the range of 75-95%, thus indicating the efficiency of the matrix elimination procedures developed. 相似文献
4.
Serotypic and genotypic characterization of human serotype 10 rotaviruses from asymptomatic neonates. 总被引:2,自引:7,他引:2
下载免费PDF全文
![点击此处可从《Journal of clinical microbiology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
S J Dunn H B Greenberg R L Ward O Nakagomi J W Burns P T Vo K A Pax M Das K Gowda C D Rao 《Journal of clinical microbiology》1993,31(1):165-169
Human rotaviruses were isolated from asymptomatic neonates at various hospitals and clinics in the city of Bangalore, India, and were found to be subgroup I specific and possess long RNA patterns (M. Sukumaran, K. Gowda, P. P. Maiya, T. P. Srinivas, M. S. Kumar, S. Aijaz, R. R. Reddy, L. Padilla, H. B. Greenberg, and C. D. Rao, Arch. Virol. 126:239-251, 1992). Three of these strains were adapted to tissue culture and found by serotype analysis and neutralization assays to be of serotype 10, a serotype commonly found in cattle but infrequently found in humans and not previously identified in neonates. By RNA-RNA hybridization, a high level of relatedness to a serotype 10 bovine rotavirus strain and a low-to-medium level of relatedness to a human rotavirus strain were observed. Since this human isolate shares a genogroup with bovine rotavirus, it is likely that it originated by interspecies transmission. A human rotavirus strain isolated from asymptomatic neonates and similar to bovine rotavirus might represent a good vaccine candidate. 相似文献
5.
I. E. Ezeagu A. G. Gopal Krishna S. Khatoon L. R. Gowda 《Ecology of food and nutrition》2004,43(4):295-305
Analysis showed the seeds of Adenanthera pavonina contained appreciable amounts of proteins (29.44g/100g), crude fat (17.99g/100g), and minerals, comparable to commonly consumed staples. Total sugar was low (8.2g/100g) while starch (41.95g/100g) constitutes the major carbohydrates. Low levels of antinutrients were reported and methionine and cystine were the most deficient amino acids. Linoleic and oleic acids make up 70.7 percent of the total fatty acids. Free fatty acid levels were relatively high but peroxide and saponification values of 29.6mEqkg-1 and 164.1mgKOHg-1respectively point to a resemblance to oils processed for food. It was concluded that A. pavonina seeds represent a potential source of oil and protein that could alleviate shortages. 相似文献
6.
M. M. Kesavulu A. S. Prakasha Gowda T. N. C. Ramya N. Surolia K. Suguna 《Chemical biology & drug design》2005,66(4):211-219
Abstract: Plasmepsin group of enzymes are key enzymes in the life cycle of malarial parasites. As inhibition of plasmepsins leads to the parasite's death, these enzymes can be utilized as potential drug targets. Although many drugs are available, it has been observed that Plasmodium falciparum, the species that causes most of the malarial infections and subsequent death, has developed resistance against most of the drugs. Based on the cleavage sites of hemglobin, the substrate for plasmepsins, we have designed two compounds (p‐nitrobenzoyl‐leucine‐β‐alanine and p‐nitrobenzoyl‐leucine‐isonipecotic acid), synthesized them, solved their crystal structures and studied their inhibitory effect using experimental and theoretical (docking) methods. In this paper, we discuss the synthesis, crystal structures and inhibitory nature of these two compounds which have a potential to inhibit plasmepsins. 相似文献
7.
Reversible myocardial dysfunction: basics and evaluation 总被引:3,自引:0,他引:3
Large areas of non-functional but viable myocardium with reversible dysfunction are commonly seen in patients with acute myocardial infarction. Both reperfusion of acutely ischemic myocardium and chronic myocardial ischemia may produce a reversible forms of ventricular dysfunction. The two main conditions that lead to reversible myocardial dysfunction are stunned myocardium and hibernating myocardium. Myocardial stunning represents post-ischemic myocardial dysfunction that persists despite restoration of normal flow, with gradual return of contractile function. Hibernating myocardium is a state of persistently impaired myocardial function at rest due to reduced coronary blood flow owing to residual stenosis that can be restored toward normal by revascularization. The success of the revascularization procedures depends on the presence of amount of dysfunctional but viable myocardium. The basics and evaluation of reversible myocardial dysfunction are reviewed. 相似文献
8.
T‐2 toxin is the most toxic among mycotoxins and poses a potential health hazard for both humans and animals. At high doses, T‐2 toxin can cause shock‐like syndrome that can result in death. We evaluated the effect of time course and route of exposure on hepatic oxidative damage in mice and it is only such study so far to compare the effects of dermal and subcutaneous exposure of T‐2 toxin. Mice were exposed to 1 LD50 of T‐2 toxin either by percutaneous (5.94 mg/kg body weight) or subcutaneous (1.54 mg/kg body weight) route and sacrificed at 0, 1, 3, and 7 days postexposure. Analysis of a number of serum biochemical variables, antioxidant enzymes activity, gene and protein expression by immunoblot assay showed time and route dependent effects of T‐2 induced hepatic oxidative damage. Time dependent increase in protein carbonyl content and protein oxidation was seen in serum and liver. Results of our study may provide possible mechanism for developing medical countermeasures against T‐2 toxin. © 2013 Wiley Periodicals, Inc. Environ Toxicol 30: 64–73, 2015. 相似文献
9.
10.
Hudson Keenihan SN Ratiwayanto S Soebianto S Krisin Marwoto H Krishnegowda G Gowda DC Bangs MJ Fryauff DJ Richie TL Kumar S Baird JK 《The American journal of tropical medicine and hygiene》2003,69(1):36-41
Immune responses directed at glycosylphosphatidylinositol (GPI) anchors of Plasmodium falciparum may offer protection against symptomatic malaria. To independently explore the effect of age on generation of the anti-GPI IgG response, we measured serum anti-GPI IgGs in a longitudinal cohort of migrant Javanese children (6-12 years old) and adults (> or = 20 years old) with equivalent numbers of exposures to P. falciparum in Papua, Indonesia. While the peak response in adults was achieved after a single infection, comparable responses in children required > or = 3-4 infections. Significantly fewer children (16%) than adults (41%) showed a high (optical density > 0.44) anti-GPI IgG response (odds ratio [OR] = 3.8, 95% confidence interval [CI] = 2.3-6.3, P < 0.0001), and adults were more likely to show a persistently high response (OR = 5.5, 95% CI = 1.0-56.8, P = 0.03). However, the minority of children showing a strong response were significantly less likely to experience symptoms with subsequent parasitemia compared with those with a weak response (OR = 4.0, 95% CI = 1.1-13.8, P = 0.02). This effect was not seen among high- and low-responding adults (OR = 1.2, 95% CI = 0.5-2.8, P = 0.60). Host age, independent of cumulative exposure, apparently represents a key determinant of the quantitative and qualitative nature of the IgG response to P. falciparum GPI. 相似文献