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van Leth F Huisamen CB Badaro R Vandercam B de Wet J Montaner JS Hall DB Wit FW Lange JM;NN Study Group 《Journal of acquired immune deficiency syndromes (1999)》2005,38(3):296-300
BACKGROUND: The initial rate of plasma HIV-1 RNA (pVL) decline has been proposed as a marker of early efficacy of antiretroviral therapy (ART) and a possible predictor of late efficacy. We compared the rate of pVL decline in patients starting ART with nevirapine (NVP), efavirenz (EFV), or both drugs combined in addition to lamivudine (3TC) and stavudine (d4T). METHODS: Analysis of the viral decay constant (VDc) during the first 2 weeks of treatment in patients enrolled in the 2NN study who remained on allocated treatment. RESULTS: The median VDc (log10 copies per day, [interquartile range]) was similar for NVP (0.30 [0.25-0.36], EFV (0.31 [0.27-0.37]), and NVP + EFV (0.30 [0.27-0.36]). Patients with a baseline pVL >100,000 copies/mL were 8.7 (95% confidence interval [CI]: 6.2-12.3) times more likely to have a VDc >75th percentile. A high VDc was not associated with plasma drug concentration or with a decreased risk of virologic failure at week 48 after the start of therapy (hazard ratio = 0.8, 95% CI: 0.6-1.2). CONCLUSION: NVP, EFV, or NVP + EFV in combination with 3TC and d4T show similar rates of pVL decline during the first 2 weeks of treatment. The VDc with these regimens is not predictive of late virologic efficacy. 相似文献
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Laboratory of Neurophysiology of Brain Integrative Activity and Section Brain and Behavior,, A. I. Karaev Institute of Physiology, Academy of Sciences of the Azerbaijan SSR, Baku. (Presented by Academician of the Academy of Medical Sciences of the USSR O. S. Adrianov.) Translated from Byulleten' Éksperimental'noi Biologii i Meditsiny, Vol. 111, No. 3, pp. 227–229, March, 1991. 相似文献
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Mureev S Kushnir S Kolesnikov AA Breitling R Alexandrov K 《Molecular and biochemical parasitology》2007,155(2):71-83
The trypanosomatid protozoan Leishmania tarentolae has been extensively used as a model system for studying causative agents of several tropical diseases and more recently as a host for recombinant protein production. Here we analyze the rates of partial or complete deletions of expression cassettes integrated into small ribosomal RNA and tubulin gene clusters as well as into ornithine decarboxylase gene of L. tarentolae. In approximately 60% of cases gene conversion was responsible for the deletion while in the rest of the cases deletion occurred within the expression cassette. We used this observation to design constitutive and inducible expression vectors that could be stably integrated into the genome and subsequently depleted of the antibiotic resistance genes using thymidine kinase or bleomycin resistance genes as negative selection markers. This enabled us to obtain L. tarentolae strains containing constitutive or inducible markerless expression cassettes. Analysis of the markerless strains demonstrated that although stability varied among clones some were stable for as many as 200 generations. We expect that this approach will be useful for the construction of strains carrying multiple expression cassettes for analysis of trypanosomatid pathogenicity mechanisms and overexpression of multi-subunit protein complexes for biochemical and structural studies. 相似文献
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Louise Chappell-Maor Masha Kolesnikov Jung-Seok Kim Anat Shemer Zhana Haimon Jonathan Grozovski Sigalit Boura-Halfon Takahiro Masuda Marco Prinz Steffen Jung 《European journal of immunology》2020,50(3):353-362
Conditional mutagenesis and fate mapping have contributed considerably to our understanding of physiology and pathology. Specifically, Cre recombinase-based approaches allow the definition of cell type-specific contributions to disease development and of inter-cellular communication circuits in respective animal models. Here we compared Cx3cr1CreER and Sall1CreER transgenic mice and their use to decipher the brain macrophage compartment as a showcase to discuss recent technological advances. Specifically, we highlight the need to define the accuracy of Cre recombinase expression, as well as strengths and pitfalls of these particular systems that should be taken into consideration when applying these models. 相似文献
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Vassiliy A. Medvedev Ilya E. Kolesnikov Pavel K. Olshin Mikhail D. Mikhailov Alina A. Manshina Daria V. Mamonova 《Materials》2022,15(7)
Optical materials doped with several lanthanides are unique in their properties and are widely used in various fields of science and technology. The study of these systems provides solutions for noncontact thermometry, bioimaging, sensing technology, and others. In this paper, we report on the demonstration of YVO4 nanoparticles doped with one, two, and three different rare earth ions (Tm3+, Er3+, and Nd3+). We discuss the morphology, structural properties, and luminescence behavior of particles. Luminescence decay kinetics reveal the energy transfer efficiency (up to 78%) for different ions under the selective excitation of individual ions. Thus, we found that the energy transition from Tm3+ is more favorable than from Er3+ while we did not observe any significant energy rearrangement in the samples under the excitation of Nd3+. The observed strong variation of REI lifetimes makes the suggested nanoparticles promising for luminescent labeling, anticounterfeiting, development of data storage systems, etc. 相似文献
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