全文获取类型
收费全文 | 1349篇 |
免费 | 57篇 |
国内免费 | 6篇 |
专业分类
耳鼻咽喉 | 6篇 |
儿科学 | 10篇 |
妇产科学 | 7篇 |
基础医学 | 161篇 |
口腔科学 | 38篇 |
临床医学 | 79篇 |
内科学 | 379篇 |
皮肤病学 | 12篇 |
神经病学 | 93篇 |
特种医学 | 44篇 |
外科学 | 194篇 |
综合类 | 8篇 |
预防医学 | 72篇 |
眼科学 | 10篇 |
药学 | 94篇 |
中国医学 | 2篇 |
肿瘤学 | 203篇 |
出版年
2023年 | 19篇 |
2022年 | 22篇 |
2021年 | 48篇 |
2020年 | 13篇 |
2019年 | 21篇 |
2018年 | 29篇 |
2017年 | 27篇 |
2016年 | 30篇 |
2015年 | 24篇 |
2014年 | 30篇 |
2013年 | 65篇 |
2012年 | 101篇 |
2011年 | 109篇 |
2010年 | 55篇 |
2009年 | 44篇 |
2008年 | 84篇 |
2007年 | 97篇 |
2006年 | 109篇 |
2005年 | 107篇 |
2004年 | 83篇 |
2003年 | 73篇 |
2002年 | 70篇 |
2001年 | 14篇 |
2000年 | 9篇 |
1999年 | 16篇 |
1998年 | 22篇 |
1997年 | 17篇 |
1996年 | 12篇 |
1995年 | 10篇 |
1994年 | 8篇 |
1993年 | 5篇 |
1992年 | 13篇 |
1991年 | 4篇 |
1989年 | 5篇 |
1988年 | 2篇 |
1987年 | 1篇 |
1986年 | 5篇 |
1985年 | 3篇 |
1982年 | 1篇 |
1981年 | 1篇 |
1974年 | 1篇 |
1966年 | 1篇 |
1965年 | 1篇 |
1963年 | 1篇 |
排序方式: 共有1412条查询结果,搜索用时 31 毫秒
1.
Kazuhiko Tsuruya Atsumi Harada Shinji Kubo Kouji Mitsuiki Kazuhito Takeda 《Clinical and experimental nephrology》1997,1(2):131-135
A 69-year-old man was admitted to our kidney center with endstage renal failure. We started intermittent peritoneal dialysis
immediately because of severe azotemia, hyperkalemia, and metabolic acidosis. Two weeks after admission, he developed uremic
pericarditis with frequent ventricular premature contractions and supraventricular premature contractions. The intermittent
peritoneal dialysis was then replaced by intensive hemodialysis, and oral administration of 300 mg/d of cibenzoline was started.
Four days later, he developed thirst, weakness, and dyspnea due to respiratory muscular paralysis. We initiated respiratory
support with a respirator because analysis of his blood gases revealed marked hypercapnia and hypoxia. He also developed hypoglycemia
and prolonged PQ and QRS intervals on the electrocardiogram, which we believed were due to cibenzoline intoxication; we discontinued
the cibenzoline immediately. All symptoms improved, and he was extubated 5 days later. After 2 months, his pericardial effusion
disappeared. He now continues maintenance hemodialysis as an outpatient. We suspect that the cibenzoline induced the respiratory
muscular paralysis for 2 reasons: 1) the patient experienced the respiratory muscular paralysis, at the same time he also
experienced thirst, weakness, hypoglycemia, and prolonged PQ and QRS intervals on electrocardiogram, and all of these symptoms
improved after the discontinuation of cibenzoline, and 2) his plasma concentration of cibenzoline became remarkably elevated,
to 20 times above the standard therapeutic level. This patient's clinical course indicates that hemodialysis might be superior
to intermittent peritoneal dialysis for treatment of cibenzoline intoxication. 相似文献
2.
Shiroh Miura Takashi Kurita Kazuhito Noda Mitsuyoshi Ayabe Hisamichi Aizawa Takayuki Taniwaki 《Journal of clinical neuroscience》2009,16(4):589-590
We describe a 53-year-old man with herpes simplex virus (HSV) brainstem encephalitis diagnosed based by positive HSV immunoglobulin M antibodies from cerebrospinal fluid. The MRI findings of this case had three unique features. First, the lesions were symmetrical. Second, the lesions may have been associated with reactivation of HSV infection in the region of the trigeminal nerve. Third, diffusion-weighted and apparent diffusion coefficient (ADC) imaging, conducted for the first time on an HSV brainstem encephalitis case, suggested that the lesions were associated with vasogenic edema. 相似文献
3.
Satoru Miyawaki Ryuichi Yamazaki Takehiko Harada Shigehiko Takanashi Tadashi Nagashima Hiroshi Nakaguchi Ryo Okazaki Kazuhito Yamazaki Yasuo Ishida Akira Matsuno 《Journal of clinical neuroscience》2007,14(5):481-484
Skull metastasis of thyroid carcinoma is rare. The majority of skull metastases of thyroid carcinoma are of the follicular subtype, rather than thyroid papillary carcinoma. In this report, a 55-year-old woman with skull metastasis from thyroid papillary carcinoma is presented. The metastatic lesion of the skull was hypervascular and osteolytic, and the bleeding was profuse during resection. There have been only four reports of skull metastasis from thyroid papillary carcinoma. The mean period from the initial diagnosis of thyroid carcinoma until the detection of skull metastasis is 23.3 years, whereas in this patient, it was about 2 years. Therefore, in the clinical course of thyroid papillary carcinoma, skull metastasis should be considered, and the patient should be meticulously followed up. 相似文献
4.
5.
Kazuhito Yamamoto Hirotaka Osada Masao Seto Michinori Ogura Hisamitsu Suzuki Kazuhiko R. Utsumi Atsushi Oyama Yutaka Ariyoshi Shigeo Nakamura Souji Kurita Toshitada Takahashi Ryuzo Ueda 《Cancer science》1992,83(5):465-476
A case of non-Hodgkin's lymphoma showed a phenotypic and genotypic cell lineage switch twice during nine years of his clinical history; first, T-cell type, pleomorphic small cell lymphoma developed, followed by B-cell type, diffuse centroblastic/centrocytic lymphoma, and finally T-zone lymphoma without follicles again developed, from which AST-1 cultured cell line was established. Karyotype analysis demonstrated a shared abnormal chromosome, der(1)t(1;?)(p36;?), among the first relapsed B-cell tumor, the second relapsed T-cell tumor and AST-1 cell line. Furthermore, T-cell receptor (TCR) γ gene rearrangement bands of the same size were observed in the first relapsed B-cell tumor and the second relapsed T-cell tumor as well as AST-1 cell line. These results suggested that both relapsed tumors of different cell lineages are derived from a common malignant clone, presumably a committed lymphoid stem cell. A unique translocation, t(2;14)(q37;q11.2), which may involve TCR δ/α gene complex, was observed in the second relapsed tumor and AST-1 cells. To attempt to isolate the breakpoint of this translocation, the configuration of TCR δ/α gene complex was studied. The result showed that two rearrangements of TCR α gene detected with Jα probes were the products of the normal TCR rearrangement process, and were not involved in the translocation at this region. This patient, together with the AST-1 cell line, provided us a unique opportunity to study the development and clonal evolution of malignant lymphoma. 相似文献
6.
S. M. Mirbod Hideyo Yoshida Marjan Jamali Kazuhito Masamura Ryoichi Inaba Hirotoshi Iwata 《International archives of occupational and environmental health》1997,70(1):22-28
The aims of this study were (1) to evaluate subjective symptoms in the hand-arm system of all traffic police motorcyclists
of a city located in the central part of Japan and (2) to assess their hand-arm vibration exposure associated with traffic
police motorcycle riding. The study population consisted of 119 motorcycling traffic policemen and 49 male controls. By means
of a questionnaire, information on the occupational history and the presence of subjective symptoms in the hand-arm system
of all subjects was obtained. Vibration was measured on the handlebars of the representative motorcycles and on the hands
of the riders. The 4- and 8-h energy-equivalent frequency-weighted acceleration as well as the lifetime vibration dose were
calculated for all police motorcyclists. The prevalence of finger blanching in the traffic police motorcyclists was 4.2%,
but none of the controls had this symptom. The rates of finger numbness (19.3%), finger stiffness (16.0%), shoulder pain (13.4%),
and shoulder stiffness (45.4%) were significantly higher among police motorcyclists as compared with controls. The root-mean-square
(rms) frequency-weighted acceleration on the handlebars of police motorcycles was in the range of 2.2–4.9 m/s2 rms. The computed 4- and 8-h energy-equivalent frequency-weighted acceleration values were 2.8– 4.5 and 2.0 –3.2 m/s2 rms, respectively. A pattern of increasing percentage prevalence with increasing cumulative vibration dose was noticed. The
subjects with a lifetime vibration dose of more than 20.1 m2 h 3 s-4 (ln scale) showed significantly higher prevalence rates for symptoms in the fingers and shoulders as compared with the control
group. As occupational vibration exposure of traffic police motorcyclists might be considered a risk factor for the development
of symptoms in the hand-arm system of the riders, its evaluation and control is needed for prevention methodology evolution.
Received: 15 April 1996/Accepted: 8 November 1996 相似文献
7.
Md. Iqbal Hossain Chowdhury Yoshio Koyanagi Michihiko Suzuki Susumu Kobayashi Kazuhito Yamaguchi Naoki Yamamoto 《Virus genes》1992,6(1):63-78
Syncytia or multinucleated giant-cell formation is one of the major cytopathic effects induced by human immunodeficiency virus (HIV) infection. Cell fusion results from the strong interaction of CD4 molecules on the surface of the uninfected T cells and gp120, an external envelope glycoprotein of HIV on the infected T cells. We studied the production of HIV in fusion cells between MOLT-4 and virus-infected MOLT-4/HIV cells and found that HIV production was enhanced up to three- to fivefold, which showed a good correlation with the appearance and extent of syncytia formation. Blocking the fusion by monoclonal antibody against a binding epitope of CD4 molecule to gp120 decreased the HIV production significantly. Enhancement of HIV production was observed by more than five-fold in comparison with chronically infected cells, which were fusion free 20 hr postcocultivation. Electron microscopic observation also showed the presence of abundant HIV particles inside the fused cells and on the outer surface. AZT blocked the HIV augmentation of fused cells in coculture completely. Southern blot analysis revealed that both integrated and unintegrated HIV DNA were highly accumulated in fusion cells, as compared with fusion-free MOLT-4/HIV cells. Among unintegrated DNA, circular and linear DNA were accumulated to a similar degree. Northern blot hybridization showed that rapid enhancement of all three species of HIV-specific RNA containing genomic (9.2 kb) and subgenomic (4.3 and 1.9 kb) RNAs were found 20 hr postinfection in fusion cells. These data suggest that syncytia formation is an extremely active infection process of HIV, by which multiple rounds of reinfection might take place. 相似文献
8.
Ueda K 《Rinsho byori. The Japanese journal of clinical pathology》2005,53(5):437-445
Circulating autoantibodies are useful diagnostic markers of cancers and autoimmune diseases. Research over the past decade has resulted in some reports on the presence of autoantibodies against disease-related proteins such as annexin-I & II, recoverin and protein gene product 9.5 in the sera of patients with lung cancer, and also against calreticulin and alpha-enolase in autoimmune diseases. In this study, we first identified the a-enolase autoantibody in the sera of patients with lung adenocarcinoma by proteomics-based analysis. The comparison of two-dimensional polyacrylamide gel electrophoresis (2D-PAGE)/western blot (WB)/ECL detection revealed distinct distributions of antibodies in the sera of lung adenocarcinoma, tuberculosis and healthy subjects which reacted with soluble proteins derived from the adenocarcinoma A549 cell line. We found 16 spots in patients with adenocarcinoma by 2D-PAGE/WB/ECL detection and identified alpha-enolase, chaperonin, and other autoantibodies in the adenocarcinoma patients' sera. The specificities of an antibody against alpha-enolase was preliminarily observed in sera from 3 of 5 patients with adenocarcinoma, 0 of 10 patients with tuberculosis and 0 of 10 healthy subjects. In conclusion, we first identified alpha-enolase autoantibody in sera of lung adenocarcinoma and the autoantibody was seemed to be a specific marker of the lung adenocarcinoma. In addition, we also identified various autoantibodies in esophageal cancer, hepatocellular carcinoma, and non-Hodgkin's lymphoma. Moreover, we tried to identify the corresponding antigen of an unknown anti-cytoplasmic autoantibody, and an anti-red blood cell antibody by proteomics-based analysis. These antibodies might become new diagnosis markers. 相似文献
9.
Tatsuo Yamazaki Shigeki Tomita Kazuhito Ichikawa Yuko Ono Fujiyuki Inaba Ichio Fukasawa Yasuo Imai Johji Imura Hirokazu Fukui Takahiro Fujimori Noriyuki Inaba 《Pathobiology》2006,73(4):176-182
OBJECTIVES: We investigated the relationship between P16-immunostaining patterns and clinicopathological factors in early uterine cervix cancers and assessed whether P16-immunostaining patterns predict the prognosis of the patients with early uterine cervix cancers. METHODS: Twenty-nine early squamous cell carcinoma (SCC) specimens of the uterus were examined using immunohistochemistry for P16 expression. The P16-immunostaining pattern was classified into two groups: the homogeneous type and the heterogeneous type. P16-immunostaining patterns were evaluated in different parts of the carcinoma in situ (CIS): the center of the tumor and the front interface of the infiltrating tumor. RESULTS: All specimens were of the homogeneous type in CIS. The P16-immunostaining pattern was significantly of the heterogeneous type in the front interface of the infiltrating tumor with lymphatic invasion, vascular invasion, lymph node metastasis, and recurrence. Regarding the P16-immunostaining patterns in the front interface of the infiltrating tumor, the patients with the heterogeneous type showed a significantly worse prognosis than the patients with the homogeneous type. CONCLUSIONS: The prognosis of patients with early uterine cervical SCC may be predicted by evaluating the P16-immunostaining pattern in the front interface of the infiltrating tumor. 相似文献
10.
Role of ubiquitin carboxy terminal hydrolase-L1 in neural cell apoptosis induced by ischemic retinal injury in vivo 总被引:7,自引:0,他引:7
下载免费PDF全文
![点击此处可从《The American journal of pathology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Harada T Harada C Wang YL Osaka H Amanai K Tanaka K Takizawa S Setsuie R Sakurai M Sato Y Noda M Wada K 《The American journal of pathology》2004,164(1):59-64
Ubiquitin is thought to be a stress protein that plays an important role in protecting cells under stress conditions; however, its precise role is unclear. Ubiquitin expression level is controlled by the balance of ubiquitinating and deubiquitinating enzymes. To investigate the function of deubiquitinating enzymes on ischemia-induced neural cell apoptosis in vivo, we analyzed gracile axonal dystrophy (gad) mice with an exon deletion for ubiquitin carboxy terminal hydrolase-L1 (UCH-L1), a neuron-specific deubiquitinating enzyme. In wild-type mouse retina, light stimuli and ischemic retinal injury induced strong ubiquitin expression in the inner retina, and its expression pattern was similar to that of UCH-L1. On the other hand, gad mice showed reduced ubiquitin induction after light stimuli and ischemia, whereas expression levels of antiapoptotic (Bcl-2 and XIAP) and prosurvival (brain-derived neurotrophic factor) proteins that are normally degraded by an ubiquitin-proteasome pathway were significantly higher. Consistently, ischemia-induced caspase activity and neural cell apoptosis were suppressed approximately 70% in gad mice. These results demonstrate that UCH-L1 is involved in ubiquitin expression after stress stimuli, but excessive ubiquitin induction following ischemic injury may rather lead to neural cell apoptosis in vivo. 相似文献