Clinical Analysis of 57 Patients with Ovarian Dysgerminoma

Objective  Ovarian dysgerminoma is an uncommon ovarian malignancy, Its clinicai features are special and there are many factors affecting its prognosis. If treated properly, the patient can be cured. Otherwise it may endanger the patient’s life. The aim of this study is to investigate the clinical features and factors related to prognosis of ovarian dysgerminoma. Methods  Data from 57 patients with pure ovarian dysgerminoma were analyzed retrospectively. The patients were admitted to the Cancer Center, Sun Yat-sen University from January 1.1964 to December 31, 2000. Results  The main clinical features were abdominal mass (56.1% ), abdominal pain (21.1% ), abdominal swelling (17.5%.), vaginal bleeding (5.3% )and genital tract abnormalities (5.3%). Twenty-six patients had stage I diseases, 8 stage II.9 stage III.1 stage IV and 13 recurrent and persistent diseases. The uterus was involved in 41.2% of patients with stage II -III diseases. Combined modality was given to 52 cases and a single-method treatment to 5 cases. The total overall 5 and 10-year survival rates for stages I-IV was 80.1 % and 70.0% respectively. The 5-year survival rate for stage I was 100%, stage II 55.2%. stage III 55.6% and stage IV 0%; for recurrent and persistent diseases, 72.7%. The stage I group of 12 patients. received adnexectomy and 14 patients underwent hysterectomy and adnexa removal. There was no significant difference between the 5 and 10-year survival rates (all 100%). Of the 23 patients in the stage I group to whom oniy chemotherapy was given after operation, 19 cases received 3 or more courses and were well without recurrence; 4 patients received only one course and one of them recurred 21 months after the operation. In the group of stages II and III cases, the 5-year survival rate was 86.7% for those whose chemotherapy courses were 3≥ 4 and 25.0% for patients who received less than 4 courses of chemotherapy (P<0.05). Conclusions  The prognosis of ovarian dysgerminoma is closely related to the disease stage and treatment modality. A fertility-preserving operation can be considered in early -staged patients, but caution needs to be exercised in the middle to late staged cases. Good results can be achieved with an operation-based combined modality in recurrent patients.     

Radical cystectomy with ileoneocystoplasty

There were examined 32 patients with cancer of bladder, to whom radical cystectomy with ileoneocystoplasty in the author's modification was performed. Operative intervention was done for the transitional cell carcinoma of bladder in T2-T4 stage. The method of the bladder formation using resection of the ileum segment 40-60 cm in length was adduced. The intestinal segment was transsected along the side opposite to mesentery, the transplant was placed in U-like fashion, the posterior wall of bladder was formed sewing together medial edges, anastomosis between bladder and ureter was performed. In 17 patients postoperative complications had occurred, mainly of septic-purulent character. Postoperative mortality was 9.4%. Radical cystectomy with ileoneocystoplasty is an effective method of medico-social rehabilitation of patients, which secures restoration of urodynamics of upper urinary ways and urination.     

The Influence of biotinylation of 2F5 antibody on peptide selection from the combinatorial phage library

The effect of biotinylation of monoclonal antibodies (MAbs) on the yield and repertoire of peptides selected by biopanning is discussed in the present study. A comparative analysis of peptides selected from a phage library using the biotinylated and nonbiotinylated MAb 2F5 was performed. The yield of the peptides homologous to the native epitope was observed to be 1.7 times higher for the biotinylated antibodies, whereas the use of the nonbiotinylated antibodies in the enzyme-linked immunoassay (ELISA) resulted in a greater binding capacity of the selected phages with MAb 2F5. It is notable that the phages, displaying peptides that have a fouror five-amino-acid sequence similarity with the native epitope, demonstrate the highest binding affinity to the antibodies. The phages exposing peptides with three-amino-acid sequence similarity exhibit various binding affinities. In summary, the results have revealed that a rational biopanning protocol should be chosen according to the aim of each particular study.     

Hygienic assessment of protective effects of overalls and individual protective devices used in the production of ampicillin trihydrate and its dosage form, ampicillin sodium salt

The working clothes and individual protective devices recommended by the industrial Rules for the production of ampicillin trihydrate and its medicinal derivatives such as natrium salt ampicillin, do not provide adequate protection of the skin and respiratory mucous from antibiotic contamination. The use of the overalls in combination with the pressurized helmet (designed and produced at the 'Sintez' enterprise) which provided air supply for breathing and, partially, under the overalls, significantly decreased contamination of the body. At the stages of manual handling of the finished products, adequate protection was attained by using the protective paste produced at the 'Altaivitamine' enterprise.     

Acute coronary syndromes

The first 150 words of the full text of this article appear below. Key points Coronary artery disease accounts for >30% ofdeaths in Western society. The diagnosis of myocardial infarctionshould be qualified by size, causation and time from occurrence. Mortalityis reduced by immediate or ‘primary’ percutaneouscoronary intervention or thrombolysis within the first 24 hof onset of ST-segment elevation myocardial infarction. Strategiesto reduce platelet activation (glycoprotein IIb/IIIa receptorantagonists, or clopidogrel) are now recommended in the treatmentof high-risk non-ST-segment myocardial infarction/unstable angina. Elevatedserum troponins may be the result of non-ischaemic myocardialdamage, especially in critical illness.