基于网格化管理的持续质量改进模式在静脉血栓栓塞症防控中的应用效果

目的 探讨基于网格化管理的持续质量改进模式(grid management-PDCA,GM-PDCA)在静脉血栓栓塞症(venous thromboembolism,VTE)防控中的应用效果,以期为提高护理质量和管理效率提供现实依据.方法 2019年1-12月,便利抽样法选取某院的住院患者为对照组,即GM-PDCA实施...     

随机光学重构显微镜在外泌体观察中的应用

目的 探讨随机光学重构显微镜（STORM）在外泌体观察中的应用价值,并介绍外泌体在随机光学重构显微镜下的成像原理及方法。 方法 实验中在肾性甲旁亢患者原代培养的甲状旁腺细胞的培养液上清中加入EXOQUICK-TC外泌体沉淀剂来获取细胞培养上清中的外泌体,将外泌体膜表面的特异性跨膜蛋白CD63作免疫荧光标记后利用随机光学重构显微镜对外泌体行超高分辨率成像并测量外泌体直径。 结果 实验中随机光学重构显微镜成功的对继发性甲旁亢甲状旁腺细胞原代培养液上清中的外泌体行单分子定位、直径测量及超高分辨率成像。 结论 因随机光学重构显微镜具有独特的光学特性并突破了光学衍射极限的限制,较传统光学显微镜,能获得20~50 nm的分辨率,可对外泌体行单分子精确定位、直径测量、超高分辨率成像。基于STORM的成像优势,相信STORM及其他超高分辨率成像技术将在外泌体及外泌体参与的生物学过程的研究中发挥重要作用。       

LncRNA MEG3 impacts proliferation,invasion, and migration of ovarian cancer cells through regulating <Emphasis Type="Italic">PTEN</Emphasis>

Objective and design

We investigated the expressions of lncRNA MEG3 and PTEN in ovarian cancer tissues and their effects on cell proliferation, cycle and apoptosis of ovarian cancer.

Methods

Expression levels of MEG3 in ovarian cancer cell lines and normal ovarian cell lines were detected by qRT-PCR. Cell viability was detected by MTT assay. Cell apoptosis and cell cycle distribution were measured by flow cytometry. Cell invasion capability was tested by transwell assay. Cell migration capacity was tested by wound healing. The xenograft model was constructed to explore the effect of lncRNA MEG3 on ovarian cancer in vivo.

Result

Compared with normal ovarian cells, expression levels of MEG3 and PTEN were relatively lower in ovarian cancer cells. There was a positive correlation between the expression of PTEN and the expression of MEG3. Enhanced expression level of PTEN suppressed SKOV3 cell proliferation, increased cell apoptosis rate, and decreased cell invasion and migration.

Conclusion

LncRNA MEG3 and PTEN were down-regulated in ovarian cancer cells. LncRNA MEG3 regulated the downstream gene PTEN in ovarian cancer cells to prohibit cell proliferation, promote apoptosis and block cell cycle progression.

     

益气健脾汤加减联合拖线引流治疗泛发性肛周脓肿临床研究

目的:观察益气健脾汤加减联合拖线引流疗法治疗泛发性肛周脓肿的临床疗效。方法:选取102例泛发性肛周脓肿患者为研究对象,按照随机数表法分为研究组与对照组各51例。对照组实施局部切开引流,研究组采取益气健脾汤加减联合拖线引流疗法。比较两组患者在治疗前及治疗2周后,其中医症候(肛周肿痛、创面焮热、溃后伤口不敛)积分变化; 记录两组患者创面愈合时间、术后2周内复发率及成瘘率差异; 分析两组患者在术后1 d、术后1周及术后2周时,其疼痛程度[数字评分法(NRS)]及肛门功能(Wexner量表)评分变化。结果:治疗2周后,两组患者中医症候积分低于治疗前(P<0.05),且研究组低于对照组(P<0.05); 研究组创面愈合时间短于对照组(P<0.05),且术后2周内复发率及成瘘率均较对照组低(P<0.05); 术后1 d时,两组患者NRS、Wexner评分比较差异无统计学意义(P>0.05),术后1周及术后2周时,两组患者肛门NRS、Wexner评分均较术后1 d时降低(P<0.05),且研究组较对照组低(P<0.05)。结论:益气健脾汤加减联合拖线引流疗法可缓解泛发性肛周脓肿患者疾病症状,加快创面愈合速度并降低复发率、成瘘率,改善患者疼痛程度与肛门功能。     

医务人员感染冠状病毒危险因素的Meta分析

目的系统评价冠状病毒所致医务人员感染的危险因素,为医务人员提供基于循证证据的感染防控方案。方法计算机检索多个中英文数据库自2002～2020年2月的文献,采用Stata12.0软件进行Meta分析。结果共纳入11项研究,4 795名医务人员。Meta分析结果显示医务人员感染冠状病毒的危险因素包含以下高风险医疗程序:气管插管[OR=4.231,95%CI(1.625,11.015)]、气管切开[OR=5.069,95%CI(2.437,10.543)]、吸痰术[OR=1.672,95%CI(1.349,2.072)]、无创通气[OR=3.140,95%CI(1.439,6.851)]、胸外按压[OR=4.500,95%CI(1.500,13.800)]。结论实施高风险医疗程序增加医务人员感染风险,医务人员应警惕相关危险因素,积极采取防护措施。     

LncRNA MEG3 impacts proliferation,invasion, and migration of ovarian cancer cells through regulating PTEN

Objective and design

We investigated the expressions of lncRNA MEG3 and PTEN in ovarian cancer tissues and their effects on cell proliferation, cycle and apoptosis of ovarian cancer.

Methods

Expression levels of MEG3 in ovarian cancer cell lines and normal ovarian cell lines were detected by qRT-PCR. Cell viability was detected by MTT assay. Cell apoptosis and cell cycle distribution were measured by flow cytometry. Cell invasion capability was tested by transwell assay. Cell migration capacity was tested by wound healing. The xenograft model was constructed to explore the effect of lncRNA MEG3 on ovarian cancer in vivo.

Result

Compared with normal ovarian cells, expression levels of MEG3 and PTEN were relatively lower in ovarian cancer cells. There was a positive correlation between the expression of PTEN and the expression of MEG3. Enhanced expression level of PTEN suppressed SKOV3 cell proliferation, increased cell apoptosis rate, and decreased cell invasion and migration.

Conclusion

LncRNA MEG3 and PTEN were down-regulated in ovarian cancer cells. LncRNA MEG3 regulated the downstream gene PTEN in ovarian cancer cells to prohibit cell proliferation, promote apoptosis and block cell cycle progression.

     

尿路结石患者掌握饮食预防知识的调查分析

目的 了解尿路结石患饮食预防知识的掌握情况。方法 对200例尿石症患进行饮食与尿石症关系认知情况、饮食习惯调查。结果 收回有效问卷180分。患对饮食与尿石症形成与复发的关系有初步的认识；患对饮食中各种具体成分对尿石症形成与复发的关系知识缺乏。结论 强调饮食预防的重要性，消除患不良的饮食习惯；饮食教育应具有针对性及长期性，才能促进尿路结石患掌握饮食预防知识。     

标准化病人联合“互联网+”多学科教学 在静疗专科护士培训中的应用

目的:探讨标准化病人联合“互联网+”多学科教学的方法在静脉治疗专科护士培训中的效果。方法:选择四川省人民医院静脉治疗专科护士学员33名为研究对象,随机分为A组17人,B组16人,分别采用标准化病人联合“互联网+”多学科教学、传统带教的临床教学。在学员结束实习时进行理论知识、操作考核,满意度调查,并将结果进行比较分析。结果:在理论考核的案例分析部分,A组得分为(23.78±1.76)高于B组得分(18.35±1.09);操作考核部分,A组得分(94.97±1.37)高于B组得分(90.79±1.59)。差异有统计学意义(P<0.05)。2组满意度调查结果显示没有差异。结论:标准化病人联合“互联网+”多学科教学有助于增强静疗专科护士的综合学习和临床实践能力。     

无缝隙护理模式在乳腺癌根治保留肋间臂神经术中的应用

目的观察在乳腺癌根治保留肋间臂神经（ICBN）术中应用无缝隙护理模式患肢功能恢复情况。方法 186例乳腺癌根治保留ICBN患者,随机分为两组,实验组97例,对照组89例,实验组按照无缝隙管理理念,给予无缝隙干预,对照组按常规指导患肢功能锻炼。结果全部病例术后1个月、2个月进行随访,实验组及对照组术后1个月患肢功能恢复率分别为80.4%及66.3%,2个月分别为89.7%及84.3%,差异有统计学意义。结论无缝隙护理模式保证了患者功能锻炼的完整性和持续性,最大程度恢复了患肢功能,提高了生活质量。