An Experimental Study on the Flexibility of Prevention against Thrombosis Following Mechanical Valve Replacement by tPA Gene Transduction

Objectives Use a gene suture immersed recombinant tissue-type plasminogen activator （r-tPA）expression plasmid to transduce myocardia to prevent the thrombosis after mechanical tricuspid valve replacement in pigs. Methods A r-tPA gene plasmid was constructed and conjugated to a novel cationic phosphonolipid and a r-tPA gene suture was made. Eighteen pigs were selected and divided into two groups at randomization. There were 9 pigs in the experimental group and 9 in the control group, all the 18 pigs＇ tricuspids were replaced with mechanical valves. The gene threads were sutured into the right ventricular walls near mechanical valves and an ultrasound was used on the surfaces of the right ventricular walls for the gene transfer in the experimental group. Coagulative function, D-dimer level of the blood and the thrombosis on the surfaces of the valves were observed. Results r-tPA gene plasmid was successfully constructed and r-tPA protein was expressed in the ventricular cells around the gene sutures. D-dimer reached its peak level （ 1.67 ±0. 79） μg · mL^-1 in 1 week after operation in two groups, but it decreased to preoperation level thereafter in control group and kept on the high level and reincreased to a new high level （ 1.89 ± 0.79 ） μg · mL^-1 until the end of the experiment in experimental group. The thromboses around the valves were found in all the control group （100%） but only 1 （ 11.11% ） case in experimental group. There were no changes in prothrombin time pre and post operation in two groups. Conclusions Using gene suture immersed r-tPA expression plasmid to transduce myocardia might be a best substitution for life long anti-coagulation therapy for the patients, who underwent operation.     

人脑胶质瘤基因表达谱芯片的构建和初步验证

目的：构建人脑胶质瘤特异的基因表达谱芯片,促进大规模胶质瘤表达谱的研究。方法：采用389条基因探针,优化基因芯片制作的各个条件：片基、点样液、点样大小、紫外交联、采集信号强度等。使用自制芯片检测20例胶质瘤标本的基因表达谱,并与以前报道的实验结果对比,验证芯片的特异性和灵敏度。结果：构建了针对人脑胶质瘤的基因表达谱芯片,并用于脑胶质瘤的基因表达谱分析,证实本产品可以有效地高通量检测相关基因的表达水平。结论：构建的胶质瘤芯片有望用于胶质瘤基因表达谱分析。     

蠕动插入式内窥镜机器人的研究

论述国外内窥镜机器人系统的组成、移动机构的模型和蠕动式机器人的应用研究,将为最小创伤性外科手术提供一种崭新的手段,对我国现代医学工程发展有参考价值。     

内镜结合锁孔技术在微血管减压术中的应用

目的 探讨神经内镜结合锁孔技术在微血管减压术治疗原发性三叉神经痛中的应用.方法 回顾性分析65例应用神经内镜结合锁孔微血管减压术治疗的原发性三叉神经痛患者的临床资料、手术资料及随访结果.手术方式为经乳突后锁孔入路,并在垫入隔片前后均置入神经内镜行多角度观察.结果 1例用神经内镜探查时发现并存的责任动脉,11例内镜探查后调整隔片位置.65例患者中术后62例(95.4%)疼痛消失,2例(3.1%)疼痛减轻,总有效率98.5%.治疗有效的64例均获得随访,随访时间8～55个月,平均34个月.术后3年内2例复发,复发率3.1%.结论 锁孔技术手术创伤小,能减少术后并发症的发生;应用神经内镜探查能避免遗漏责任血管、协助调整隔片位置,提高手术有效性,降低术后复发率.     

Jumonji domain containing-3 (JMJD3) inhibition attenuates IL-1β-induced chondrocytes damage in vitro and protects osteoarthritis cartilage in vivo

Objectives

This study aimed to explore the effects and relative mechanism of JMJD3 on knee osteoarthritis (OA).

Methods

In this study, we first analyzed the expression of JMJD3 in OA cartilage using western blot and immunohistochemistry. In an in vitro study, the effects of GSK-J4, JMJD3 inhibitor, on ATDC-5 chondrocytes were evaluated by CCK-8 assay. Real-time PCR and western blot were used to examine the inhibitory effect of GSK-J4 on the inflammation and ECM degradation of chondrocytes. NF-κB p65 phosphorylation and nuclear translocation were measured by western blot and immunofluorescence. In the animal study, twenty mice were randomized into four experimental groups: sham group, DMM-induced OA + DMSO group, OA + low-dose GSK-J4 group, and OA + high-dose GSK-J4 group. After the treatment, hematoxylin–eosin and safranin O/fast green staining were used to evaluate cartilage degradation of knee joint, with OARSI scores for quantitative assessment of cartilage damage.

Results

Our results revealed that JMJD3 was overexpressed in OA cartilage and GSK-J4 could suppress the IL-1β-induced production of pro-inflammatory cytokines and catabolic enzymes, including IL-6, IL-8, MMP-9 and ADAMTS-5. Consistent with these findings, GSK-J4 could inhibit IL-1β-induced degradation of collagen II and aggrecan. Mechanistically, GSK-J4 dramatically suppressed IL-1β-stimulated NF-κB signal pathway activation. In vivo, GSK-J4 prevented cartilage damage in mouse DMM-induced OA model.

Conclusions

This study elucidates the important role of JMJD3 in cartilage degeneration in OA, and our results indicate that JDJM3 may become a novel therapeutic target in OA therapy.

     

不伴有出血性脑挫伤的成人急性创伤性硬膜下血肿预后因素分析

目的 分析不伴有出血性脑挫伤的成人急性创伤性硬膜下血肿患者的各项临床指标与预后，为预测预后提供依据。方法 用回归分析和X^2检验对70例患者各项临床指标与其预后进行统计分析。结果 GCS评分、受伤后意识变化、入院时瞳孔情况、年龄、受伤原因、CT影像学表现（鞍上池显示不良或闭塞、合并蛛网膜下腔出血、血肿部位、中线移位程度）对预后产生影响。结论 上述九项指标可作为预测预后的临床指标，尤以前八项影响预后更显著。     

过氧化物酶体增殖因子活化受体γ在垂体腺瘤中的表达及其意义

目的：观察过氧化物酶体增殖因子活化受体γ（PPARγ）在垂体腺瘤组织中的表达情况,分析PPAR7与垂体腺瘤激素免疫分型等之间的关系。方法：选取2002年1月-2005年5月38例手术切除的垂体腺瘤,利用RT—PCR和Western Blot技术分析PPARγ在垂体腺瘤中的表达情况及其与垂体腺瘤激素免疫分型等之间的关系。结果：所有肿瘤均发现有PPARγ的表达。按照垂体腺瘤激素免疫分型各组肿瘤表达的PPARγmRNA和PPARγ蛋白表达水平均无显著差异。结论：人类垂体腺瘤中有PPAR7的表达。     

乙酰肝素酶反义寡核苷酸对A375细胞增殖及乙酰肝素酶蛋白表达的影响

目的:观察乙酰肝素酶反义寡核苷酸(ASODN)对人恶性黑素瘤A375细胞增殖及乙酰肝素酶蛋白表达的影响.方法:以空白组和无关序列(N-ODN)组为对照,设计、合成4条ASODN链(ASODN-t1,t2,t3和t4,脂质体包埋后分别以10 μmol/L、20 μmol/L、30 μmol/L N-ODN和ASODN转染A375细胞,台盼蓝排斥试验检测细胞增殖情况,免疫组织化学法检测乙酰肝素酶蛋白表达的变化.结果:转染72 h后,各ASODN组A375细胞计数较对照组、N-ODN组均减少(P均=0.000);每条链的10 μmol/L、20 μmol/L、30 μmol/L 3个浓度组相比,差异均有统计学意义(P均=0.000);30 μmol/L ASODN-t2组与其他各组相比,差异均有统计学意义(P均＜0.05).转染48 h后,30 μmol/L ASODN-t2组乙酰肝素酶蛋白的表达较对照组、N-ODN组下调(P均=0.000).结论:乙酰肝素酶ASODN可抑制A375细胞增殖,下调乙酰肝素酶蛋白的表达.     

STS gene in a pedigree with X-linked ichthyosis

To investigate the gene mutation in a pedigree with X-linked iehthyosis （XLI） and to explore the relationship between the mutation and its clinical manifestations, genomic DNA of affected members, the normal member of the pedigree and 50 unrelated normal members was extracted with a whole blood genomie DNA extraction kit and the DNA was used as a template for the polymerase chain reaction （PCR） mediated amplification of exon 1 and exon 10 of the STS gene. hHb6 （human hair basic keratin） gene was used as the internal control. Our results showed that the STS gene was deleted in affected members in the pedigree with X-linked ichthyosis. The normal member of the pedigree and 50 unrelated normal members had no such deletion. The proband and his mother had products in the internal control after PCR amplification. The blank control had no product. It is concluded that deletion of the STS gene existed in this pedigree with X-linked iehthyosis, and it is responsible for the unique skin lesions of X-linked ichthyosis.     

桑叶总黄酮对体外蛋白糖基化的抑制作用

目的 研究桑叶总黄酮对非酶糖基化的抑制作用.方法 从桑叶中提取不同浓度(0.001,0.01,0.1g/L)总黄酮加入体外白蛋白糖基化体系中,分别孵育1,4,7,14,21,28天,测定早期糖化白蛋白产物的浓度.结果 糖化蛋白产量与孵育时间呈正相关,桑叶总黄酮(01g/L)在各时间点均使糖化蛋白生成较对照组少.结论 蛋白质非酶糖化与葡萄糖孵育时间正相关,桑叶总黄酮有可能阻断蛋白非酶糖化.