MiR-200c-3p inhibits cell migration and invasion of clear cell renal cell carcinoma via regulating SLC6A1

In this study, we investigated the mechanism of miR-200c-3p and SLC6A1 in regulating cell activity of clear cell renal cell carcinoma (CCRCC). The mRNA and miRNA expressions of tissue specimens were analyzed by CapitalBio Corporation (Beijing, China). The expression of SLC6A1 in CCRCC cells was examined through qRT-PCR and western blot. The migration and invasion ability of 786-O cells was testified by transwell assay after transfected. 786-O cell proliferation ability was detected by MTT assay. Dual luciferase reporter assay verified the association between SLC6A1 and miR-200c-3p. SLC6A1 was high expressed and miR-200c-3p was low expressed in CCRCC tissues and cells. Besides, lower SLC6A1 expression indicated longer survival time and higher survival rate. MiR-200c-3p could directly target at SLC6A1 and reduce its expression. MiR-200c-3p inhibited the proliferation, migration and invasion in 786-O cells by down-regulating SLC6A1 expression. The results suggested that the miR-200c-3p served as a suppressor for CCRCC via down-regulating SLC6A1.     

Zinc finger of the cerebellum 5 promotes colorectal cancer cell proliferation and cell cycle progression through enhanced CDK1/CDC25c signaling

IntroductionColorectal cancer (CRC), mostly caused by external or environmental factors, is the third most common and lethal cancer worldwide. Although a large number of investigations have been carried out to reveal the evolution of CRC, the underlying mechanisms of CRC remain unclear.Material and methodsExpression of zinc finger of the cerebellum 5 (ZIC5) in CRC tissues and cell models was measured by qRT-PCR and IHC. Cell transfection was carried out for ZIC5 overexpression or knockdown. The MTT assay was applied to examine the capacity of glioma cell proliferation. Wound healing assay and tumor invasion assay were used to test the capacity of glioma cell migration and invasion respectively. Cell cycle analysis and western blot were used to verify the apoptosis rates of CRC cells upon ZIC5 overexpression or downregulation. A further tumor Xenograft study was used to examine the effects of ZIC5 on tumor malignancy in vivo.ResultsCell models using HCT116 and SW620 cells were established to study the ZIC5 function upon ZIC5 overexpression of knockdown. Consistently, we discovered that ZIC5 also significantly increased in Chinese CRC patients. In addition, ZIC5 promoted CRC cell proliferation through increasing the proportion of cells maintained in the S phase. ZIC5 overexpression facilitated the capacity of CRC cell migration and invasion. Inhibition of ZIC5 mitigated such malignant effects.ConclusionsCollectively, investigations of the ZIC5 in CRC provided a new insight into CRC diagnosis, treatment, prognosis and next-step translational therapeutic developments from bench to clinic.     

良性前列腺增生合并膀胱结石的腔内治疗

目的：探讨气压道道／超声碎石术和经尿道前列腺汽化电切术治疗前列腺增生（BPH）合并膀胱结石的治疗效果。方法：采用瑞士气压弹道／超声碎石清石系统（lithoclast Rmaster）和经尿道前列腺汽化电切设备治疗前列腺增生合并膀胱结石43例。结果：41例一次手术成功,手术中碎石时间15～60min,平均32min;前列腺电切时间50～110min,平均85min。术中术后均无输血,未发生前列腺电切综合征、膀胱穿孔等并发症。术后5～6d拔除尿管,未发生排尿困难、尿失禁等。病理结果均为良性前列腺增生（BPH）。国际前列腺症状评分由术前（21．4士2．06）分下降至术后（7．4±1．2）分（t=2．182,P〈0．05）,最大尿流率由术前（6．37±1．96）ml／s升至（19．05±1．95）ml／s（t=29．9904,P〈0．0000）,生活质量评分由术前（4．76±0．94）分术降至为（2．25士0．75）分（t=13．626,P〈0．0000）。结论：气压弹道／超声碎石术和经尿道前列腺汽化电切术能有效治疗BPH合并膀胱结石。     

Homocysteine might increase the risk of recurrence in patients presenting with primary cerebral infarction

Purpose: Although hyperhomocysteinemia (Hhcy) is a risk factor for cerebral infarction, its effect on recurrent cerebral infarction is less-defined. We aimed to investigate the association of Hhcy and increased risk of recurrent cerebral infarct.

Materials and methods: From 2011 to 2013, we recruited 231 primary cerebral infarct patients that were divided to a Hhcy group (n?=?105) and a control group (n?=?126) according to plasma homocysteinemia (Hcy) levels exceeding 15?μmol/L. In this prospective study, risk factors such as gender, age, blood lipid and glucose levels, history of diabetes, high blood pressure, smoking habits and plasma Hhcy levels were determined. A three-year follow-up compared differences in cerebral infarction recurrence rates. Statistical analyses identified whether plasma Hhcy levels were an independent risk factor for recurrent cerebral infarction.

Results: Triglyceride and low-density lipoprotein (LDL) levels in the Hhcy group were significantly higher than controls, and cerebral infarct recurrence rates in the Hhcy group exceeded control subject rates through the three-year follow-up (p?=?.021, p?=?.036 and p?=?.025). Cox proportional hazards modeling showed that elevated Hhcy levels (hazard ratio [HR]?=?3.062, p?<?.001), increased age (HR?=?1.069, p?<?.01), circulating triglyceride levels (HR?=?1.686, p?=?.048), and relative National Institutes of Health Stroke (NIHSS) score (HR?=?1.068, p?=?.016) were risk factors for recurrent cerebral infarction.

Conclusions: Level of Hhcy was a risk factor for recurrent cerebral infarction. Further, particular demographic and clinical outcomes including age, relative NIHSS scores, and circulating triglyceride levels were markedly associated with the occurrence of cerebral infarction.     

内镜辅助下小切口术式在泌尿外科中的应用

目的：评价内镜辅助下小切口术式的临床应用效果。方法：对42例患者行内镜辅助下小切口手术，其中根治性肾切除2例。单纯性肾切除3例（结核肾，无功能肾），盂管成形术3例，肾盂输尿管切开取石术2例，肾囊肿去顶减压术17例，肾上腺肿瘤切除15例。18例肾上腺肿瘤行腹腔镜手术。结果：根治性肾切除或单纯性肾切除手术时间平均120min，术中出血量约100ml；盂管成形术、肾盂或输尿管切开取石术。术中出血量约50-80ml；肾囊肿去顶减压术手术时间平均30-60min，肾上腺肿瘤切除手术时间平均110min，术中出血量约50-80ml。内镜辅助下小切口泌尿外科手术术中出血量、术后住院时间、手术切口长度与腹腔镜组比较差异无统计学意义；在手术时间和住院费用方面则优于腹腔镜组，差异有统计学意义。结论：内镜辅助下小切口泌尿外科手术具有创伤小、价格低、无需专门设备及人员培训的特点，易于推广普及，为泌尿外科微创手术提供了新方式。     

新疆维吾尔族与汉族载脂蛋白A5c.553G＞T基因多态性分布特征及与血脂的关系

目的 研究载脂蛋白A5(apolipoproteinA5,ApoA5)c.553G ＞T多态性在新疆维吾尔族(维族)及汉族人群的分布特征及与血脂的关系.方法 采用聚合酶链反应-限制性片段多态性(PCR-RFLP)方法,对406例维族和527例汉族健康体检者ApoA5基因c.553G＞T多态性进行检测.结果 ApoA5基因c.553G＞T的3种基因型在维族组的分布频率分别为:GG型378例(93.1％),GT型27例(6.7％),TT型1例(0.25％);在汉族组的分布频率分别为:GG型478例(90.7％),GT型49例(9.3％),TT型无,两组基因型分布差异无统计学意义(P＞0.05).维族组及汉族组中,T等位基因携带者(TT +GT基因型)较GG型的甘油三酯(TG)水平高[维族:(2.24±1.13)mmol/L比(1.69±1.12)mmol/L;汉族:(2.41±1.38)mmol/L比(1.69±1.40)mmol/L,P＜0.01].Logistics回归分析校正了性别、年龄、吸烟饮酒史、BMI、血压、血脂等影响因素后,在维、汉两组T等位基因携带者较GG型患高甘油三酯血症的风险仍有明显增高(维族组:OR 3.31,95％ CI 1.31～8.36;汉族组:OR 3.98,95％ CI 1.81～8.74).结论 ApoA5基因c.553G＞T多态性分布频率在新疆维、汉民族之间没有差异,该多态性与血清TG水平有关,T等位基因是高甘油三酯血症的一个危险因素.     

维生素D受体基因多态性与新疆南疆地区维吾尔族小儿上尿路含钙结石易感性的关系研究

目的：探讨维生素D受体（VDR）基因多态性与新疆南疆地区维吾尔族小儿上尿路含钙结石的关系。方法选择新疆南疆地区维吾尔族上尿路含钙结石患儿89例（病例组）和同地区正常儿童121例（对照组）,提取外周血基因组DNA,应用聚合酶链反应-限制性片段长度多态性方法检测并分析 VDR的ApaI、TaqI、FokI基因多态性的分布频率及其与小儿上尿路含钙结石的关系。结果病例组和对照组 VDR基因的Fok I等位基因多态性分布频率差异有统计学意义（χ2＝15．04;P ＜0．001）,两组ApaI和TaqI等位基因多态性分布频率差异无统计学意义（χ2＝0．350,P ＝0．552;χ2＝2．930,P ＝0．093）;两组FokI的基因型差异有统计学意义（χ2＝11．621,P ＜0．001）,两组ApaI和TaqI的基因型分布频率差异无统计学意义（χ2＝0．287,P ＝0．592;χ2＝2．483,P ＝0．115）。FokI基因型的分布频率在男性中有统计学意义（χ2＝8．497,P ＝0．004）,在女性中无统计学意义（χ2＝3．230,P ＝0．072）;ApaI和TaqI基因型分布频率无论在男性还是女性中均没有统计学意义（χ2＝1．938,P ＝0．164;χ2＝0．828,P ＝0．363和χ2＝1．667,P＝0．197;χ2＝0．937,P ＝0．333）。结论维生素D受体（VDR）的FokI基因多态性可作为新疆维吾尔族小儿上尿路含钙结石的基因标记物,f等位基因可作为含钙结石危险因素的标志;ApaI和TaqI基因多态性与含钙结石的形成没有关系。     

活体肾移植供者安全性的随访观察

目的探讨活体肾移植对供者安全性的影响。方法研究对象为2003年4月至2007年4月在新疆医科大学第一附属医院完成活体供肾肾移植的62例供者。记录供者手术时间和住院时间,观察术后并发症发生情况和预后情况。术后随访,随访内容包括血清肌酐(Scr)、血尿素氮(BUN)、肾小球滤过率(GFR)、血浆白蛋白、血红蛋白及血压等指标,同时调查供肾对生活工作的影响。结果62例供者供肾手术均获成功。供者中1例开放取肾术中发生气胸,1例发生切口脂肪液化坏死,经对症治疗后痊愈。2例术后并发肺部感染,选用敏感抗生素及雾化吸入治疗后治愈,其余术后均恢复顺利。62例供者平均术后住院时间(8.2±2.6)d,随访时间为(3.2±1.1)年。所有供者均存活,家庭生活和日常工作也未受到明显影响。62例供者术前及术后7 d、3个月、1年、3年、5年的Scr、BUN、GFR、血浆白蛋白、血红蛋白及血压变化情况比较,差异无统计学意义(均为P0.05)。结论活体供肾肾切除手术安全可行。严格完善术前检查,术中仔细操作及术后严密随访对于保障供者的安全有十分重要的意义。     

Clinical outcome of exercise therapy and early post-operative rehabilitation for treatment of neglected Achilles tendon rupture: a randomized study

Purpose

Treatment of neglected Achilles tendon rupture is very challenging. This randomized study aimed to compare the clinical outcome of early post-operative rehabilitation (EPR) with post-operative cast immobilization (PCI).

Methods

Fifty-seven patients with neglected Achilles tendon rupture were randomized to receive EPR (n = 26) or PCI (n = 31) management following surgery. Clinical outcome was monitored by follow-up at weeks 8, 12, 18 and 26 and year 2. The significance of intergroup differences from the Leppilahti scoring system (LSS), ultrasonography, multislice spiral computerized tomography (MSCT) and electromyography was assessed.

Results

Ultrasonography and MSCT revealed no occurrence of tendon elongation or adhesion. Four patients could perform sustained single-leg heel-raise exercise for 60 s at post-operative day 40. The PCI group also showed increased post-operative LSS score, but recovery was slower. Post-operative complications, such as ankle joint ankylosis and osteoporosis, only occurred in the PCI group.

Conclusions

Compared with cast immobilization, early post-operative rehabilitation results in better clinical outcome and faster overall tendon regeneration of neglected Achilles tendon rupture.

Level of evidence

II.

     

旋后外旋型踝关节骨折手术治疗体会 （附41例报告）

目的探讨分析如何提高旋后外旋型踝关节骨折的手术疗效.方法回顾分析41例旋后外旋型踝关节骨折的手术方法及随访结果,患者手术时在X光机监视下先复位、固定外踝,再固定下胫腓关节,最后固定内踝,内翻位石膏固定治疗三角韧带损伤.在后踝骨折大于1/3关节面时,行螺钉固定.结果 41例患者均得到随访,平均随访时间18月(12～62月),临床疗效优25例(61%),良10例(24.4%),可4例(9.7%),差2例(4.9%).结论正确的手术方法可获得较好的临床手术疗效.