miR-21对骨质疏松小鼠骨髓基质细胞增殖的影响

目的探讨miR-21对骨质疏松小鼠骨髓基质细胞(BMSCs)增殖的影响。方法采用双侧卵巢切除法构建骨质疏松小鼠模型(VOX),分离、培养、纯化小鼠BMSCs并采用si PORT Neo FX转染pre-miR-21、pre-miR-negative control(pre-miR-NC)、antmiR-21、ant-miR-negative control(ant-miR-NC)并进行RT-PCR验证,MTT法检测小鼠BMSCs增殖情况、茜素红与碱性磷酸酶染色法检测小鼠BMSCs成骨能力、Western-blotting检测细胞增殖、成骨分化相关蛋白水平。结果骨质疏松症小鼠BMSCs中miR-21相对表达水平低于Ctrl组(P0.05),OVX-pre-miR-21组BMSCs中miR-21相对表达水平、细胞增殖、PCNA水平、Ki-67水平、ALP染色程度、ALP活性、茜素红染色程度、Runx2水平、Osterix水平均高于OVX-pre-miR-NC组(P0.05),OVX-premiR-NC组BMSCs中miR-21相对表达水平、细胞增殖、PCNA水平、Ki-67水平、ALP染色程度、ALP活性、茜素红染色程度、Runx2水平、Osterix水平均显著低于Ctrl-pre-miR-NC(P0.05); OVX-ant-miR-21组BMSCs中miR-21相对表达水平、细胞增殖、PCNA水平、Ki-67水平、ALP染色程度、ALP活性、茜素红染色程度、Runx2水平、Osterix水平均显著低于OVX-ant-miR-NC组(P0.05),OVX-ant-miR-NC组BMSCs中miR-21相对表达水平、细胞增殖、PCNA水平、Ki-67水平、ALP染色程度、ALP活性、茜素红染色程度、Runx2水平、Osterix水平均显著低于Ctrl-ant-miR-NC(P0.05)。结论提高miR-21表达水平可促进骨质疏松小鼠BMSCs增殖能力与成骨分化能力。     

Development of a dual-labeled,hydrolysis probe-based,real-time quantitative PCR assay for detection of both genotypes of duck circovirus-1 (DuCV-1) and DuCV-2

Virus Genes - In this study, we developed a real-time quantitative polymerase chain reaction (qPCR) assay based on a dual-labeled hydrolysis probe to simultaneously detect both duck circovirus...     

探讨染色体微阵列分析技术在产前诊断中的应用

目的 探讨染色体微阵列分析(CMA)技术在产前诊断中的临床应用价值。方法 选择2018年7月至2019年6月在滨州医学院附属医院行羊膜腔穿刺的132例单胎孕妇为研究对象,采用传统染色体核型分析技术与CMA技术对所有病例进行检测,并分析比较两种方法所得出的检测结果。 结果 本研究中染色体核型分析检出1例染色体不平衡变异与13例胎儿非整倍体,CMA检测结果与之相同,但CMA可更精确地给出染色体不平衡变异的异常位点。此外,在3例染色体核型正常的胎儿中,CMA亦检出致病性基因的拷贝数变异,从而对疾病的诊断率提高2.27％。在4例核型正常的胎儿中,CMA检出临床意义不明确的拷贝数变异(CNV),检出率为3.03％;但CMA不能检测出染色体倒位、多态性及平衡易位。 结论 对非整倍体和不平衡染色体变异的检出率,CMA与核型分析相同,但CMA的敏感性和分辨率更高,同时亦可检出其他有临床意义的基因拷贝数。但因CMA技术自身亦有局限性,尚不可完全替代传统染色体核型分析技术。     

1例急性淋巴瘤细胞白血病并发肿瘤溶解综合征的药学监护

探讨临床药师参与肿瘤溶解综合征的药物治疗的方法.即从药物选择、药物相互作用、饮食指导等方面参与治疗1例肿瘤溶解综合征的过程.在临床药师的协助下,患者得到很好的救治,顺利出院.     

Aqueous leaching of lithium from simulated pyrometallurgical slag by sodium sulfate roasting

In the pyrometallurgical treatment for spent lithium-ion batteries (LIBs), lithium is generally present in slag with Al, Ca and Si and is hard to be further treated. In this study, lithium was recovered from a simulated pyrometallurgical slag (pyro-slag) via sodium roasting and water leaching. The thermodynamic process for the reactions between slag and additives such as NaCl, NaNO₃ and Na₂SO₄ were simulated during roasting by the HSC software, where Na₂SO₄ possessed stronger chemical reactivity. The optimal conditions for roasting were experimentally determined to be 800 °C for 60 min and an Na₂SO₄/Li molar ratio of 3 : 1, followed by water leaching at 70 °C for 80 min using a liquid-to-solid (L/S) mass ratio of 30 : 1. This yielded a maximum of 93.62% lithium recovery. The mechanism by which insoluble lithium in slag was transformed into soluble lithium by salt roasting was proposed using the analysis of XRD and EDS spectra, in which ion exchange occurred between Na⁺ and Li⁺ at a certain temperature.

An effective method for the recovery of lithium from the slag formed by treating spent lithium-ion batteries.