豚鼠脑干听觉中枢和内侧膝状体GABAA受体β亚单位的分布

目的：研究豚鼠脑干听觉中枢内侧膝状体ＧＡＢＡＡ受体β亚单位的分布。方法：采用免抗ＧＡＢＡＡ受体β亚单位血清和免疫组织化学ＡＢＣ法及葡萄糖氧化酶－ＤＡＢ－镍成色技术。     

Isolation and differentiation of embryonic stem cells from BALB/c mouse

Objective To invest the efficient method which can culture and induce embryonic stem cells to neurocyte in vitro. Methods Isolate the blastula of 3.5 d from BALB/c species mouse. Culture the cells from inner cell mass (inner cell mass, ICM) which were isolated by mechanical method on the mouse embryonic fibroblaste cell (MEF) feeder layer or 0.1% gelatin coated dishes. The stem cells were identified by characterized morphology, alkaline phosphatase stain, differential potency in vivo and immunochemistry stain. The isolated cells were differentiated by serial induction method that mimicking the intrinsic developmental process of the neural system. Results The isolated cells were positive for alkaline phosphatatse and SSEA-1 (stage specific embryonic antigen 1). Moreover they were identified pluripotent by differentiation in vivo. Therefore the isolated cells presented the characters of ESCs. Then the isolated cells were able to differentiate into neurocytes in vitro. Conclusion Mouse embryonic stem cells isolation, culture and differentiation system has been established.     

RNAi沉默HMGA1基因对肝癌细胞凋亡和细胞周期的影响

目的:筛选HMGA1基因沉默稳定转染肝癌细胞株,检测沉默后肿瘤细胞凋亡、周期和增殖的变化。方法:靶向HMGA1的RNAi载体转染肝癌细胞,G418筛选得到稳定转染细胞株并鉴定;MTT法和FACS检测细胞增殖、凋亡及细胞周期。结果:成功建立基因沉默HMGA1稳定细胞株;HMGA1基因沉默后,细胞凋亡百分率(29.46±3.04%)明显高于质粒对照组和空白对照组(1.96±0.76%和2.04±0.70%,P<0.01);G0-G1期细胞百分率(75.21±2.35%)明显高于质粒对照组和空白对照组(37.98±3.02%和39.23±3.63%,P<0.01),G2-S期(24.79±2.35%)显著低于质粒对照组和空白对照组(62.03±3.01%和60.77±3.63%,P<0.01);MTT检测显示,HMGA1沉默细胞增殖与质粒对照组和空白对照组相比显著降低(P<0.01或0.05)。结论:基因沉默HMGA1可促进肿瘤细胞凋亡,抑制细胞增殖。     

常熟市≥35岁人群肥胖测量指标与糖尿病患病关系研究

目的 探讨肥胖测量指标BMI(体质指数)、WC(腰围)、WHR(腰臀比)、WHtR(腰身比)与糖尿病患病的关系.方法 采用多阶段整群随机抽样的方法,随机抽取6个乡镇/街道≥35岁的居民11599名进行问卷调查并体检,分析BMI、WC、WHR、WHtR和糖尿病的关系.结果 常熟市≥35岁成人糖尿病患病率12.18％,空腹血糖平均水平为(5.66±1.32)mmol/L.不同BMI、WC、WHR、WHtR组间空腹血糖水平差异均有统计学意义(P值均＜0.05);BMI、WC、WHR、WHtR与空腹血糖水平呈正相关关系;BMI、WC、WHR、WHtR最高分位组糖尿病患病风险分别是最低分位组的4.40、2.50、2.26、2.13倍(P值均＜0.05).BMI、WC、WHR、WHtR每增加1个标准差,糖尿病患病风险分别增加40.0％、50.4％、52.3％、46.2％.结论 肥胖测量指标BMI、WC、WHR、WHtR与糖尿病关系密切,糖尿病患病风险随着肥胖程度的增加而升高,中心性肥胖指标对糖尿病的筛查价值优于BMI.     

体外受精—胚胎移植术后异位妊娠29例临床分析

目的 :探讨体外受精 -胚胎移植后异位妊娠的发生率及高危因素。方法 :对我院 1998年 9月～ 2 0 0 3年 3月接受体外受精 -胚胎移植后发生的异位妊娠 2 9例进行回顾性分析。结果 :异位妊娠发生率为 5 .8%。 2 9例异位妊娠中有 19例术前存在输卵管病变。结论 :体外受精 -胚胎移植术后发生异位妊娠的高危因素是输卵管病变、继发性不孕 ,年龄、子宫位置、移植术过程的困难程度等亦与之有关     

动物尿和骨灰样中硼测定方法的研究

目的　建立测定动物尿和骨灰中硼含量的方法。方法　样品经前处理后 ,用氢氟酸氟化 ,将尿和骨灰样中的硼转化成氟硼酸盐 ,在一定的酸度下 ,直接用氟硼酸根离子选择电极测定。结果　该方法在 0 .5～ 10 0 .0 μg/ml范围内呈线性响应 ,直线回归方程 ^Y =2 4 3.16 - 5 6 .4 9X ,相关系数r=- 0 .9998,检出限为 0 .0 6 μg/ml,精密度测定相对标准偏差小于 4 % ,加标回收率 90 .3% ,均符合方法学要求。同时分析了灌服含硼抗氟剂的大鼠尿、骨灰硼含量 ,与对照组比较 ,差异有显著性 (P <0 .0 1)。结论　该方法稳定性强、选择性好、经济简便 ,具有较强的使用价值。     

Electrical coupling synchronises spinal motoneuron activity during swimming in hatchling Xenopus tadpoles

The role of electrical coupling between neurons in the swimming rhythm generator of Xenopus embryos has been studied using pharmacological blockade of gap junctions. A conspicuous effect of 18β-glycyrrhetinic acid (18β-GA) and carbenoxolone, which have been shown to block electrical coupling in this preparation, was to increase the duration of ventral root bursts throughout the spinal cord during swimming. The left-right coordination, the swimming frequency and the duration of swimming episodes were not affected by concentrations of 18β-GA which significantly increased burst durations. However, the longitudinal coupling was affected such that 18β-GA led to a significant correlation between rostrocaudal delays and cycle periods, which is usually only present in older larval animals. Patch clamp recordings from spinal motoneurons tested whether gap junction blockers affect the spike timing and/or firing pattern of motoneurons during fictive swimming. In the presence of 18β-GA motoneurons continued to fire a single, but broader action potential in each cycle of swimming, and the timing of their spikes relative to the ventral root burst became more variable. 18β-GA had no detectable effect on the resting membrane potential of motoneurons, but led to a significant increase in input resistance, consistent with the block of gap junctions. This effect did not result in increased firing during swimming, despite the fact that multiple spikes can occur in response to current injection. Applications of 18β-GA at larval stage 42 had no discernible effect on locomotion. The results, which suggest that electrical coupling primarily functions to synchronize activity in synergistic motoneurons during embryo swimming, are discussed in the context of motor system development.     

颅内动脉粥样硬化的预后

颅内动脉粥样硬化可能存在种族差异，白人颅内动脉粥样硬化性狭窄的发生率较低，发生卒中的风险也较低；而亚洲人颅内动脉粥样硬化性狭窄发生率较高，是卒中复发和血管事件相关性死亡的重要预测因素。经皮腔内血管成形术和支架置入术联合药物治疗能起到较好的预防卒中复发的作用。     

苯那普利和尼群地平对高血压病患者肾血流动力学及微量蛋白的影响

为探讨苯那普利和尼群地平对高血压病患者肾血清动力学和微量蛋白的作用，本文观察了５８例高血压病患者服用苯那普利和尼群地平前后肾血浆流量及微量蛋白的变化。结果发现，治疗后苯那普利组患者有效肾血浆流量上升，肾小球滤过分数下降，尿微量蛋白减少（Ｐ均〈０．０１），尼群地平患者的此三项检查无明显变化（Ｐ〉０．０５）。结果提示，苯那普利对高血压病患者肾功能的改善作用倨于尼群地平。