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排序方式: 共有142条查询结果,搜索用时 15 毫秒
1.
Jiang WZ Jin NY Li ZJ Zhang LS Wang HW Zhang YJ Han WY 《第二军医大学学报》2006,27(4):434-434
To express the core protein of HIV-1 of Chinese prevalent strain (HIV-1 (CN)) in Pichia pastoris, the fulllength gag gene was inserted into the secretory expression vector pHILS1. Linearized recombinant plasmid pHILGAG by Sail was electrotransformed into the yeast strain GS115, and the yeast transformants were identified by PCR. To induce the interest protein to be expressed, the PCR positive transformants were inoculated in the medium of BMGY and BMMY, mRNA of the strain was detected by RT-PCR, and the expressed protein was analyzed by SDS-PAGE, Western blotting and thin layer scanning. mRNA (1.3 kb) was amplified by RT-PCR. SDS-PAGE and Western blotting analysis showed that the molecular mass of the expressed protein was 55 kD, which was similar to the expected value, and the expressed protein could react with McAb to HIV-1 p24. Thin layer scanning analysis demonstrated that the whole amount of the expressed protein was approximately 13 % of the soluble protein in the supernatant. The recombinant yeast had good genetic stability. The optimal expression conditions of the engineering yeast were as follows: BMMY medium, 80-90% of dissolved oxygen, 1% methanol, and 3-day-cultivation course. Gag proteins were expressed under the optimal expression condition and purified via gel filtration chromatography. The purity of the interest protein was up to 85 %. After the purified proteins were inoculated into BALB/c mice, the anti-HIV-1 antibodies in the immunized mice could be detected by Western blotting. 相似文献
2.
目的对自闭症病人给予被动性语言刺激,评价采用功能性磁共振(MR)成像作为判断病人有无语言缺陷的客观指标的可行性。材料与方法本研究为前瞻性研究,研究方 相似文献
3.
Golovenko NY Kravchenko IA Zin'kovskii VG Andronati SA Aleksandrova AI Ovcharenko NV Larionov VB 《Bulletin of experimental biology and medicine》2000,130(12):1153-1155
We studied the rate of phenazepam absorption into the blood and its transport to the brain from a transdermal therapeutic system and bioavailability of the drug in this system. Hydrogel matrix consisting of polyvinyl alcohol and 1,2-propylene glycol was used for application. Transdermal application of 0.1-0.4 mg phenazepam in a dose of 14 mg/kg provided a stable level of this drug during application interval (1-48 h), while its bioavailability for blood plasma and brain was 0.63 and 0.2, respectively (determined for 0.4 mg phenazepam). The rate of drug penetration into the blood and brain was 46 and 60 ng/ml/h, respectively. 相似文献
4.
O. V. Zhuk N. Ya. Golovenko V. G. Zin'kovskii 《Bulletin of experimental biology and medicine》1988,105(5):631-634
Department of Physicochemical Pharmacology, A. V. Bogatskii Physicochemical Institute, Academy of Sciences of the Ukrainian SSR, Odessa. (Presented by Academician of the Academy of Medical Sciences of the USSR A. V. Val'dman.) Translated from Byulleten' Éksperimental'noi Biologii i Meditsiny, Vol. 105, No. 5, pp. 529–531, May, 1988. 相似文献
5.
V. G. Zin'kovskii N. Ya. Golovenko O. V. Zhuk 《Bulletin of experimental biology and medicine》1988,106(4):1444-1446
Department of Physicochemical Pharmacology, A. V. Bogatskii Physicochemical Institute, Academy of Sciences of the Ukrainian SSR, Odessa. (Presented by Academician of the Academy of Medical Sciences of the USSR A. V. Val'dman.) Translated from Byulleten' Éksperimental'noi Biologii i Meditsiny, Vol. 106, No. 10, pp. 451–452, October, 1988. 相似文献
6.
7.
Kravchenko IA Golovenko NY Larionov VB Aleksandrova AI Ovcharenko NV 《Bulletin of experimental biology and medicine》2003,136(6):579-581
We studied the effect of lauric acid on transdermal penetration of phenazepam in vivo. It was found that treatment with lauric acid 3-fold increased the maximum anticonvulsive effect of phenazepam applied in a transdermal therapeutic system in comparison with the control. Study of the pharmacokinetics of phenazepam transdermal therapeutic system showed its higher bioavailability in the presence of lauric acid (f=0.9). 相似文献
8.
V G Zin'kovski? N Ia Golovenko E A Stankevich O V Zhuk A V Lisiutin 《Farmakologiia i toksikologiia》1987,50(6):65-68
The kinetic scheme of distribution of a structural analogue of phenazepam 7-brom-5-phenyl-1,2-dihydro-3H-1,4-benzdiazepine-2-on (I) and its main metabolites in the mouse body was presented. The process parameters were compared with the parameters of mouse body distribution of other N1-unsubstituted derivatives of 1,4-benzdiazepine. It was shown that for the original preparation fatty tissue, liver, spleen, myocardium may be referred to the central compartment of the kinetic scheme of distribution. No organ or tissue functioning as the peripheral compartment of the kinetic scheme of the drug distribution was found. The gastrointestinal tract of the experimental animals can serve as the peripheral compartment of the kinetic scheme for products of biotransformation of I. 相似文献
9.
N. Ya. Golovenko I. A. Kravchenko V. G. Zin'kovskii S. A. Andronati A. I. Aleksandrova N. V. Ovcharenko V. B. Larionov 《Bulletin of experimental biology and medicine》2000,130(6):1153-1155
We studied the rate of phenazepam absorption into the blood and its transport to the brain from a transdermal therapeutic
system and bioavailability of the drug in this system. Hydrogel matrix consisting of polyvinyl alcohol and 1,2-propylene glycol
was used for application. Transdermal application of 0.1–0.4 mg phenazepam in a dose of 14 mg/kg provided a stable level of
this drug during application interval (1–48 h), while its bioavailability for blood plasma and brain was 0.63 and 0.2, respectively
(determined for 0.4 mg phenazepam). The rate of drug penetration into the blood and brain was 46 and 60 ng/ml/h, respectively.
Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 130, No. 12, pp. 633–635, December, 2000 相似文献
10.