全文获取类型
收费全文 | 8637篇 |
免费 | 519篇 |
国内免费 | 63篇 |
专业分类
耳鼻咽喉 | 51篇 |
儿科学 | 251篇 |
妇产科学 | 252篇 |
基础医学 | 1156篇 |
口腔科学 | 286篇 |
临床医学 | 729篇 |
内科学 | 2145篇 |
皮肤病学 | 175篇 |
神经病学 | 910篇 |
特种医学 | 252篇 |
外科学 | 964篇 |
综合类 | 16篇 |
一般理论 | 1篇 |
预防医学 | 453篇 |
眼科学 | 141篇 |
药学 | 548篇 |
中国医学 | 8篇 |
肿瘤学 | 881篇 |
出版年
2023年 | 70篇 |
2022年 | 179篇 |
2021年 | 257篇 |
2020年 | 150篇 |
2019年 | 191篇 |
2018年 | 213篇 |
2017年 | 164篇 |
2016年 | 206篇 |
2015年 | 229篇 |
2014年 | 306篇 |
2013年 | 414篇 |
2012年 | 652篇 |
2011年 | 645篇 |
2010年 | 371篇 |
2009年 | 332篇 |
2008年 | 569篇 |
2007年 | 548篇 |
2006年 | 518篇 |
2005年 | 526篇 |
2004年 | 435篇 |
2003年 | 419篇 |
2002年 | 418篇 |
2001年 | 140篇 |
2000年 | 116篇 |
1999年 | 128篇 |
1998年 | 88篇 |
1997年 | 67篇 |
1996年 | 62篇 |
1995年 | 65篇 |
1994年 | 46篇 |
1993年 | 32篇 |
1992年 | 81篇 |
1991年 | 64篇 |
1990年 | 58篇 |
1989年 | 61篇 |
1988年 | 42篇 |
1987年 | 45篇 |
1986年 | 57篇 |
1985年 | 30篇 |
1984年 | 24篇 |
1983年 | 21篇 |
1982年 | 20篇 |
1981年 | 17篇 |
1980年 | 10篇 |
1979年 | 11篇 |
1975年 | 12篇 |
1973年 | 9篇 |
1969年 | 10篇 |
1968年 | 16篇 |
1966年 | 10篇 |
排序方式: 共有9219条查询结果,搜索用时 15 毫秒
1.
Rizzo Manglio Miguel Bluthgen Mara Virginia Recondo Gonzalo Naveira Martin Perfetti Aldo Rizzi Florencia Kuzminin Alejandro Faura Victoria Cerini Matas Videla Alejandro Silva Carlos Lupinacci Lorena Minatta Nicols 《International journal of clinical oncology / Japan Society of Clinical Oncology》2021,26(6):1057-1064
International Journal of Clinical Oncology - Immune-checkpoint inhibitors (ICIs) are standard treatments for metastatic non-small cell lung cancer (NSCLC). Patients with poor performance status... 相似文献
2.
3.
Borrelli Enrico Grosso Domenico Vella Giovanna Sacconi Riccardo Battista Marco Querques Lea Zucchiatti Ilaria Prascina Francesco Bandello Francesco Querques Giuseppe 《Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie》2020,258(12):2621-2628
Graefe's Archive for Clinical and Experimental Ophthalmology - To estimate the impact of delayed care during the coronavirus disease 2019 (COVID-19) pandemic on the outcomes of patients with... 相似文献
4.
5.
6.
7.
8.
P A DeSimone C A Martin C B Bacdayan J S Macdonald F J Rizzo J Engelberg 《Hospital practice (Office ed.)》1986,21(1):158, 160, 165-158, 7 passim
9.
10.
Francesco Bertoni Giovanni Cazzaniga Giovanna Bosshard Enrico Roggero Renzo Barbazza Michele De Boni Carlo Capella Ennio Pedrinis Franco Cavalli rea Biondi & Emanuele Zucca 《British journal of haematology》1997,97(4):830-836
Gastric MALT lymphoma usually develops from chronic gastritis, the vast majority of which (>90%) is associated with Helicobacter pylori infection. We sequenced the third complementarity determining region (CDR3) of immunoglobulin heavy chain genes in 19 gastric MALT lymphoma clones to determine the pattern of variable (V), diversity (D) and joining (J) gene utilization during immunoglobulin gene rearrangement.
DNA was extracted from paraffin-embedded sections and the rearranged CDR3 regions were amplified using a semi-nested polymerase chain reaction (with primers complementary to the conserved framework-three segment of the variable region [FR3A] and J regions). The DNA used for cloning and sequencing was obtained after purification of monoclonal bands excised from polyacrylamide gels. The N-D-N region specific to each clone was compared with known germline D sequences.
Similarly to that observed in normal and leukaemic B cells, our series of gastric MALT lymphomas showed apparent preferential utilization of genes from the DXP family. In two cases no similarity between the CDR3 nucleotide sequences of the neoplastic clones and the known germline D sequences could be found. In 10/19 analysed alleles the lymphoma B-cell clones appeared to contain two D gene segments (D-D recombination), a rare occurrence in normal individuals but one which has been described as a significant event in the determination of idiotype expression and antigen-binding affinity. Remarkably, despite the use of different D and J segments, the resultant amino acid sequences matched in two patients, suggesting the presence of a common selecting antigen.
The observed pattern of D gene rearrangement suggests that MALT lymphoma B-cell clones have undergone antigen selection, which seems to indicate that the antigen stimulation plays a pivotal role in the development of the lymphoma. 相似文献
DNA was extracted from paraffin-embedded sections and the rearranged CDR3 regions were amplified using a semi-nested polymerase chain reaction (with primers complementary to the conserved framework-three segment of the variable region [FR3A] and J regions). The DNA used for cloning and sequencing was obtained after purification of monoclonal bands excised from polyacrylamide gels. The N-D-N region specific to each clone was compared with known germline D sequences.
Similarly to that observed in normal and leukaemic B cells, our series of gastric MALT lymphomas showed apparent preferential utilization of genes from the DXP family. In two cases no similarity between the CDR3 nucleotide sequences of the neoplastic clones and the known germline D sequences could be found. In 10/19 analysed alleles the lymphoma B-cell clones appeared to contain two D gene segments (D-D recombination), a rare occurrence in normal individuals but one which has been described as a significant event in the determination of idiotype expression and antigen-binding affinity. Remarkably, despite the use of different D and J segments, the resultant amino acid sequences matched in two patients, suggesting the presence of a common selecting antigen.
The observed pattern of D gene rearrangement suggests that MALT lymphoma B-cell clones have undergone antigen selection, which seems to indicate that the antigen stimulation plays a pivotal role in the development of the lymphoma. 相似文献