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Sivaparvathi Marupudi Yamamoto Masaaki Nicolson Garth L. Gokaslan Ziya L. Fullert Gregory N. Liotta Lance A. Sawaya Raymond Rao Jasti S. 《Clinical & experimental metastasis》1996,14(1):27-34
Recent studies suggest that cysteine proteinase cathepsin L is involved in the process of tumor invasion and metastasis. We
examined cathepsin L activity in brain tumor tissue samples by an enzymatic assay, and cathepsin L protein content by enzyme-linked
immunoadsorbent assays and Western blotting to determine whether increased levels of cathepsin L correlate with the progression
of human gliomas. Native and acid-activatable cathepsin L activities were highest in glioblastomas followed by anaplastic
astrocytomas and were lowest in low-grade gliomas and normal brain tissues. Significantly higher amounts of an M
r
29 000 cathepsin L were present in glioblastomas and anaplastic astrocytomas than in normal brain tissues and low-grade glioma
tissue extracts. Using specific antibodies to cathepsin L, we also studied its cellular distribution by immunohistochemical
procedures. Higher diffuse cathepsin L immunoreactivity was found in glioblastomas than in low-grade gliomas and normal brain
tissue samples. Finally, the addition of cathepsin L antibody inhibits the invasion of glioblastoma cell lines through Matrigel
invasion assay. These results suggest the expression of cathepsin L is dramatically upregulated in malignant gliomas and correlates
with the malignant progression of human gliomas in vivo. 相似文献
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