Metabolism of lymphocytes in mice with Ehrlich ascites carcinoma

The pattern of metabolic processes in lymphocytes of mice with Ehrlich ascites carcinoma depended on the stage of tumor growth.Translated from Byulleten Eksperimentalnoi Biologii i Meditsiny, Vol. 138, No. 11, pp. 563–566, November, 2004     

The development of resistance in different inbred strains of mice to infection with Nematospiroides dubius

Infection by the intestinal nematode parasite Nematospiroides dubius was studied in seven different inbred mouse strains. Although there was some minor variation in the susceptibility of the different strains to a primary infection there were marked differences in their ability to develop resistance to infection following repeated exposure to infective larvae. The strains of mice which developed the best resistance also expelled adult worms arising from the previous infections. The adult worms resulting from a primary infection were slowly eliminated in two inbred strains studied whereas no loss occurred from outbred LACA mice. Although males and females of two strains, C3H/HeJ and CBA/H were equally susceptible to a primary infection, the females developed better resistance than the male mice following two oral administrations of third stage larvae. Infected mice of every strain and both sexes contained high levels of IgG1 in the serum.     

Correlation of extremity safety and method of reconstructive surgery on femoral-popliteal segment

Long-term results of 660 reconstructive surgeries on the arteries of femoral-popliteal segment have been analyzed. In critical limb ischemia surgeries with PTFE grafts are most effective - in 5 years 77.1% of extremities were saved compared with 69.6% after autovenous reconstructions. Use of gelatinized velour dacron (GVD) grafts (when PTFE or autovenous grafts are absent) permits to save 62.4% of extremities in 5 years. Endarterectomies (EAE) and bypass surgeries with fluorolavsan (FL) grafts should not be regarded as surgeries of choice in critical limb ischemia. Safety of limbs after surgeries with PTFE, GVD and autovenous grafts performed in the stage of claudication does not differ significantly. Cumulative safety of extremities after EAE and bypass with FL grafts is worse but high. In the first month after surgery 29.3% of all amputations were performed, in the first six months - 47.8%.     

Importance of urgent histological investigation in surgical treatment of biliopancreatoduodenal zone carcinoma

Urgent intraoperative investigation allows a surgeon, depending on the morphological conclusion, to conduct individual surgical intervention in the form of anastomosis with penetration into the tissue of the pancreas. The method decreases the rate of surgical complications, improves immediate results of patient treatment and reduces postoperative lethality. This proves the importance of morphology in the surgical treatment of tumours in the zone concerned.     

骨骼肌缺血再灌注损伤及发病机制初探

目的：探讨骨骼肌缺血再灌注损伤过程的微循环变化。组织学改变以及多肽含量变化和意义。方法：42只雄性大鼠随机分为留伴行神经组和去伴行神经组,建立标准骨骼肌缺血再灌注模型,采用激光多谱勒及显微放大分析系统,组织学方法以及凝胶电泳方法等观察缺血再灌注损伤变化。结果：缺血再灌注损伤后的骨骼肌微血管管径在20分钟时恢复率基本达到高峰约60％,此后为平台期：90分钟最高峰,为75％,主干血管流速率亦在20分钟基本达到上限,病理检查显示：缺血的骨骼肌纤维呈空泡状,核形态增大,染色加深,红细胞严重聚集,凝胶蛋白电泳提示：缺血骨骼肌中分子量20KD左右的多肽显著增加。结论：骨骼肌缺血再灌注损伤发生后,骨骼肌的微循环发生不同程度的破坏,及因之导致骨骼结构损伤和异常的20KD多肽含量明显增加,以及红细胞凝聚,白细胞的改变,这些共同构成了缺血再灌注损伤机制。     

The significance of microproteinuria for the diagnosis of kidney involvement in hypertensive disease and secondary forms of arterial hypertension]

N-acetyl-beta-glucosaminidase (NAG) activity, the concentrations of microalbumin (MA) and B2-microglobulin (B2-MG) were measured in urine of 50 healthy subjects and 200 patients suffering from arterial hypertension (AH) with preserved renal function, including patients with essential hypertension (EH), stages I and II, chronic pyelonephritis (CPN), chronic glomerulonephritis (CGN) and vasorenal hypertension (VRH). The healthy subjects, the patients with stage II EH, and those with secondary forms of AH demonstrated significant differences in NAG activity in urine. A positive correlation (r = +0.53; p < 0.03) was discovered between systolic AP and NAG activity in urine of EH patients. The concentration of MA in urine of CGN and VRH patients was significantly higher than that in the healthy subjects, EH and CPN patients. The patients with CPN and VRH showed significantly higher levels of B2-MG in urine.