荧光原位杂交技术分析人结肠菌群方法研究

建立荧光原位杂交技术分析人体内结肠菌群的方法。取受试者新鲜粪便 ,选用 5种特异性的 16SrRNA寡核苷酸探针 ,检测粪便样本收集后的保存时间、温度 ,离心条件及样本固定液存放时间对杂交计数结果的影响。结果建立最佳实验条件为 :粪便样本收集后应尽快在 4℃下保存 ,放置时间不要超过 12小时即作处理 ;样本的适宜离心条件为 70 0g 2分钟 ;样本用多聚甲醛固定后在 - 80℃下存放时间不要超过 5个月。该方法具有较好的稳定性 ,可以有效地检出个体之间结肠菌群的差异。     

A Norwegian nationwide quality assurance project in nuclear medicine: total performance in bone scintigraphy measured with a new transmission phantom

A prototype version of a recently developed transmission phantom for simulation of radionuclide bone studies was used in a Norwegian nationwide quality assurance project. The design of the phantom made it possible to perform a receiver operation characteristic (ROC) examination with respect to the detection of radionuclide accumulation in the ribs. The participants were also asked to report accumulation in the spinal column. Images obtained by means of a uniform source and a four-quadrant bar pattern were used to judge resolution and homogeneity with the collimator used in the bone studies. The overall performance of the laboratories was satisfactory, but considerable variations were found. There was a marked correlation between the physician's performance and the resolution and homogeneity of the camera. Reports from stationary imaging were generally better than those that were based on whole-body scans.