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排序方式: 共有366条查询结果,搜索用时 93 毫秒
1.
Interprofessional collaboration and communication in nursing homes: a qualitative exploration of problems in medical care for nursing home residents – study protocol
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Zhang Y Siebert R Matthiesen P Harder S Theile M Scherneck S Schlegelberger B 《Virchows Archiv : an international journal of pathology》2000,436(3):271-275
For the first time, combined immunophenotyping and fluorescence in situ hybridization (FISH) technique according to the ”fluorescence
immunophenotyping and interphase cytogenetics as a tool for investigation of neoplasms” (FICTION) technique have been successfully
applied in solid tumors. Thus, we were able to visualize the antigen expression of cells with chromosomal deletions of a tumor
suppressor region directly. In six breast carcinoma cell lines, we investigated the correlation between estrogen receptor
(ER) expression status and deletions of the estrogen receptor gene (ESR). To screen for deletions of the ESR gene, dual-color
FISH was performed with a YAC (yeast artificial chromosome) probe containing the ESR gene and, as internal control, with a
centromeric probe of chromosome 6. Deletions of the ESR gene were detected in four of six cell lines. For direct comparison
of ER expression with the copy number of the ESR gene at the single cell level, immunophenotyping with mouse anti-human ER
antibody was combined with FISH with the YAC probe containing the ESR gene according to the FICTION technique. There was no
correlation between lack of or reduced ER expression and deletions of the ESR gene. One cell line with deletions of the ESR
gene did express ER on the protein level, while another cell line without a deletion did not. Cells with deletions of the
ESR gene were either ER expression positive or negative. The staining intensity of ER expression was not associated with the
copy number of the ESR gene. Thus, this FICTION study unequivocally shows that deletions of the ESR gene are not the major
cause of absent or reduced ER expression in breast carcinoma cell lines.
Received: 6 September 1999 / Accepted: 14 September 1999 相似文献
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Coronary artery aneurysms are uncommon, usually associated with atherosclerosis and rarely involve all 3 main coronary arteries. Sudden death from documented thrombosis within large coronary aneurysms has been rarely reported. The authors report a case of a previously healthy 36-year-old male who presented with myocardial infarction complicated by sudden cardiac death. The patient was successfully resuscitated, and coronary angiography revealed diffuse, severe aneurysmal disease without evidence of atherosclerosis. A thrombus was visualized in a large aneurysm of the proximal left anterior descending artery, and there was total occlusion of a second diagonal branch, presumably due to thrombus embolization. The patient had no history of Kawasaki disease, and evaluation revealed no inflammatory or autoimmune condition. Optimal treatment and prognosis for patients with nonatherosclerotic coronary aneurysms remains unclear. Our patient was treated medically with chronic warfarin and low-dose aspirin therapy and recovered without complication. 相似文献
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Lewis W Day BJ Kohler JJ Hosseini SH Chan SS Green EC Haase CP Keebaugh ES Long R Ludaway T Russ R Steltzer J Tioleco N Santoianni R Copeland WC 《Laboratory investigation; a journal of technical methods and pathology》2007,87(4):326-335
POLG is the human gene that encodes the catalytic subunit of DNA polymerase gamma (Pol gamma), the replicase for human mitochondrial DNA (mtDNA). A POLG Y955C point mutation causes human chronic progressive external ophthalmoplegia (CPEO), a mitochondrial disease with eye muscle weakness and mtDNA defects. Y955C POLG was targeted transgenically (TG) to the murine heart. Survival was determined in four TG (+/-) lines and wild-type (WT) littermates (-/-). Left ventricle (LV) performance (echocardiography and MRI), heart rate (electrocardiography), mtDNA abundance (real time PCR), oxidation of mtDNA (8-OHdG), histopathology and electron microscopy defined the phenotype. Cardiac targeted Y955C POLG yielded a molecular signature of CPEO in the heart with cardiomyopathy (CM), mitochondrial oxidative stress, and premature death. Increased LV cavity size and LV mass, bradycardia, decreased mtDNA, increased 8-OHdG, and cardiac histopathological and mitochondrial EM defects supported and defined the phenotype. This study underscores the pathogenetic role of human mutant POLG and its gene product in mtDNA depletion, mitochondrial oxidative stress, and CM as it relates to the genetic defect in CPEO. The transgenic model pathophysiologically links human mutant Pol gamma, mtDNA depletion, and mitochondrial oxidative stress to the mtDNA replication apparatus and to CM. 相似文献
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Simsek Mert Kocer Ali Mert Cevik Seda Sen Emine Elgin Ufuk 《Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie》2020,258(7):1493-1501
Graefe's Archive for Clinical and Experimental Ophthalmology - To evaluate vascular microcirculation changes of the optic nerve head (ONH) in the patients with asymmetric pseudoexfoliative... 相似文献
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Walter Fiedler Joerg Chromik Stefanie Amberg Maxim Kebenko Felicitas Thol Vera Schlipfenbacher Anne Christine Wilke Franziska Modemann Melanie Janning Hubert Serve Arnold Ganser Carsten Bokemeyer Susann Theile Ute Deppermann Anne L. Kranich Michael Heuser 《British journal of haematology》2020,190(3):e169-e173
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Heterochromatin formation plays an important role in gene regulation and the maintenance of genome integrity. Here we present results from a study of the D. melanogaster gene vig, encoding an RNAi complex component and its homolog vig2 (CG11844) that support their involvement in heterochromatin formation and/or maintenance. Protein null mutations vigEP812 and vig2PL470 act as modifiers of Position Effect Variegation (PEV). VIG and Vig2 are present in polytene chromosomes and partially overlap with HP1. Quantitative immunoblots show depletion of HP1 and HP2 (large isoform) in isolated nuclei from the vigEP812 mutant. The vig2PL470 mutant strain demonstrates a decreased level of H3K9me2. Pull-down experiments using antibodies specific to HP1 recovered both VIG and Vig2. The association between HP1 and both VIG and Vig2 proteins depends on an RNA component. The above data and the developmental profiles of the two genes suggest that Vig2 may be involved in heterochromatin targeting and establishment early in development, while VIG may have a role in stabilizing HP1/HP2 chromatin binding during later stages. 相似文献