全文获取类型
收费全文 | 79篇 |
免费 | 11篇 |
专业分类
儿科学 | 1篇 |
妇产科学 | 1篇 |
基础医学 | 13篇 |
口腔科学 | 1篇 |
临床医学 | 2篇 |
内科学 | 12篇 |
皮肤病学 | 4篇 |
神经病学 | 17篇 |
特种医学 | 16篇 |
外科学 | 3篇 |
综合类 | 1篇 |
预防医学 | 4篇 |
眼科学 | 4篇 |
药学 | 9篇 |
中国医学 | 1篇 |
肿瘤学 | 1篇 |
出版年
2023年 | 2篇 |
2021年 | 1篇 |
2020年 | 2篇 |
2018年 | 2篇 |
2015年 | 4篇 |
2014年 | 2篇 |
2013年 | 1篇 |
2012年 | 9篇 |
2011年 | 4篇 |
2009年 | 2篇 |
2008年 | 3篇 |
2007年 | 2篇 |
2005年 | 7篇 |
2004年 | 3篇 |
2003年 | 3篇 |
2002年 | 4篇 |
2000年 | 2篇 |
1999年 | 1篇 |
1998年 | 1篇 |
1997年 | 1篇 |
1996年 | 2篇 |
1995年 | 3篇 |
1994年 | 1篇 |
1993年 | 1篇 |
1992年 | 3篇 |
1991年 | 3篇 |
1990年 | 2篇 |
1987年 | 2篇 |
1986年 | 2篇 |
1985年 | 2篇 |
1984年 | 1篇 |
1982年 | 1篇 |
1979年 | 2篇 |
1978年 | 1篇 |
1977年 | 1篇 |
1974年 | 1篇 |
1973年 | 1篇 |
1971年 | 2篇 |
1968年 | 1篇 |
1967年 | 1篇 |
1966年 | 1篇 |
排序方式: 共有90条查询结果,搜索用时 31 毫秒
1.
2.
Isolation and functional analysis of a human 70,000-dalton heat shock protein gene segment. 总被引:21,自引:0,他引:21 下载免费PDF全文
R Voellmy A Ahmed P Schiller P Bromley D Rungger 《Proceedings of the National Academy of Sciences of the United States of America》1985,82(15):4949-4953
A human 70-kDa heat shock protein (hsp70) gene segment has been isolated. The segment contains 3.15 kilobase pairs (kbp) of 5' nontranscribed sequence, an RNA leader of 119 bp, and a protein-coding region of 741 bp. The human protein sequence shows a high degree of homology to hsp70 sequences from other species. Expression experiments in Xenopus oocytes and mammalian cells indicate that a region that includes only 105 bp of 5' nontranscribed sequence contains all elements required for the efficient heat-controlled expression of the human gene. Two adjacent identical sequence elements, which are partly homologous to the Drosophila "heat shock consensus" sequence, are located 57 to 76 bp upstream from the capping site. Interestingly, the capping site itself is flanked by inverted repeat sequences. 相似文献
3.
4.
Duri K Gumbo F Kristiansen K Mapingure M Munjoma M Chirenje M Rusakaniko S Stray-Pedersen B Műller F 《AIDS research and human retroviruses》2012,28(8):885-893
To characterize phylogenetic relatedness of plasma HIV-1 RNA subtype C env gp120 viral variants capable of establishing an infection following heterosexual and subsequent vertical transmission events a 650-base pair fragment within the C2-V5 subregion was sequenced from four HIV-1-infected families each consisting of biological parent(s), index children (first), and subsequent (second) siblings. None of the family members had received antiretroviral therapy at the time of sample collection. Sequence alignment and analysis were done using Gene Doc, Clustal X, and MEGA software programs. Second siblings' sequences were homogeneous and clustered in a single branch while first siblings' sequences were more heterogeneous, clustering in separate branches, suggestive of more than one donor variants responsible for the infection or evolution from founder variant(s) could have occurred. While the directionality for heterosexual transmission could not be determined, homogeneous viral variants were a unique characteristic of maternal variants as opposed to the more heterogeneous paternal variants. Analysis of families' sequences demonstrated a localized expansion of the subtype C infection. We demonstrated that families' sequences clustered quite closely with other regional HIV-1 subtype C sequences supported by a bootstrap value of 86%, confirming the difficulty of classifying subtype C sequences on a geographic basis. Data are indicative of several mechanisms that may be involved in both vertical and heterosexual transmission. Larger studies are warranted to address the caveats of this study and build on the strengths. Our study could be the beginning of family-based HIV-1 intervention research in Zimbabwe. 相似文献
5.
Heat-regulated expression of the hepatitis B virus surface antigen in the human Wish cell line 总被引:2,自引:0,他引:2
M Dreano X Fouillet J Brochot J M Vallet M L Michel D Rungger P Bromley 《Virus research》1987,8(1):43-59
The DNA fragment coding for the hepatitis B virus surface antigen (HBsAg) was placed under the control of a human 70 kDa heat-shock protein (hsp70) promotor sequence. This plasmid construct has been used in transfection experiments to establish a stable amnion cell line of human origin (Wish), expressing an HBsAg in a heat-regulated fashion. Post-translational modifications, such as assembly, glycosylation, secretion and production of both major and middle S proteins appear to function normally. In addition, production of HBsAg under various protocols of heat induction is described. After inoculation into nude mice, development of tumours has been observed at the site of injection. Tumour cells, dispersed by means of collagenase or trypsin treatment from excised tumours, and subsequently seeded into Petri dishes, were able to secrete the same quantities of HBsAg after heat induction as were cells of the original cell line. 相似文献
6.
7.
8.
9.
10.