全文获取类型
收费全文 | 886篇 |
免费 | 35篇 |
国内免费 | 4篇 |
专业分类
耳鼻咽喉 | 8篇 |
儿科学 | 61篇 |
妇产科学 | 15篇 |
基础医学 | 99篇 |
口腔科学 | 50篇 |
临床医学 | 83篇 |
内科学 | 188篇 |
皮肤病学 | 13篇 |
神经病学 | 35篇 |
特种医学 | 154篇 |
外科学 | 58篇 |
综合类 | 5篇 |
预防医学 | 71篇 |
眼科学 | 3篇 |
药学 | 50篇 |
中国医学 | 6篇 |
肿瘤学 | 26篇 |
出版年
2023年 | 5篇 |
2022年 | 6篇 |
2021年 | 20篇 |
2020年 | 14篇 |
2019年 | 16篇 |
2018年 | 23篇 |
2017年 | 12篇 |
2016年 | 12篇 |
2015年 | 19篇 |
2014年 | 37篇 |
2013年 | 46篇 |
2012年 | 43篇 |
2011年 | 54篇 |
2010年 | 44篇 |
2009年 | 32篇 |
2008年 | 30篇 |
2007年 | 24篇 |
2006年 | 12篇 |
2005年 | 23篇 |
2004年 | 12篇 |
2003年 | 19篇 |
2002年 | 8篇 |
2001年 | 11篇 |
2000年 | 19篇 |
1999年 | 13篇 |
1998年 | 33篇 |
1997年 | 28篇 |
1996年 | 26篇 |
1995年 | 26篇 |
1994年 | 29篇 |
1993年 | 19篇 |
1992年 | 8篇 |
1991年 | 7篇 |
1990年 | 13篇 |
1989年 | 17篇 |
1988年 | 19篇 |
1987年 | 15篇 |
1986年 | 10篇 |
1985年 | 18篇 |
1984年 | 8篇 |
1983年 | 10篇 |
1982年 | 20篇 |
1981年 | 13篇 |
1980年 | 10篇 |
1978年 | 4篇 |
1977年 | 8篇 |
1976年 | 14篇 |
1975年 | 8篇 |
1970年 | 2篇 |
1908年 | 1篇 |
排序方式: 共有925条查询结果,搜索用时 15 毫秒
1.
Acute appendicitis: CT and US correlation in 100 patients 总被引:19,自引:1,他引:18
2.
Perforated colorectal neoplasms: correlation of clinical, contrast enema, and CT examinations 总被引:2,自引:0,他引:2
Hulnick DH; Megibow AJ; Balthazar EJ; Gordon RB; Surapenini R; Bosniak MA 《Radiology》1987,164(3):611-615
Results of clinical, contrast enema (CE), and computed tomographic (CT) examinations in 39 patients with perforated colorectal neoplasms were retrospectively reviewed. Twenty patients were toxemic at initial presentation, but in only four patients was the diagnosis of perforated colorectal neoplasm initially suspected clinically. CE study was performed in 22 patients and enabled the diagnosis of perforated neoplasm in 11 cases, neoplasm alone in eight, and neither neoplasm nor perforation in three. CT was performed in 38 patients and enabled the diagnosis of perforated neoplasm in 36; pericolic phlegmon but no mass lesion was evident in two. In 16 patients, CT also demonstrated metastatic disease. Because of its reliability in establishing the diagnosis and staging the extent of the inflammatory and neoplastic disease, CT is indicated in cases of suspected or proved perforated colorectal neoplasm and in cases in which CE study findings are indeterminate or suggestive of perforated neoplasm. 相似文献
3.
4.
Steenbergen EJ; Verhagen OJ; van Leeuwen EF; van den Berg H; von dem Borne AE; van der Schoot CE 《Blood》1995,86(2):692-702
Crosslineage T-cell receptor delta (TCR delta) rearrangements are widely used as tumor markers for the follow up of minimal residual disease in childhood B-precursor acute lymphoblastic leukemia (ALL) by polymerase chain reaction (PCR). The major drawback of this approach is the risk of false-negative results due to clonal evolution. We investigated the stability of V delta 2D delta 3 rearrangements in a group of 56 childhood B-precursor ALL patients by PCR and Southern blot analysis. At the PCR level, V delta 2D delta 3-to-J alpha rearranged subclones (one pathway for secondary TCR delta recombination) were demonstrated in 85.2% of V delta 2D delta 3-positive patients tested, which showed that small subclones are present in the large majority of patients despite apparently monoclonal TCR delta Southern blot patterns. Sequence analysis of V delta 2D delta 3J alpha rearrangements showed a biased J alpha gene usage, with HAPO5 and J alpha F in 26 of 32 and 6 of 32 clones, respectively. Comparison of V delta 2D delta 3 rearrangement status between diagnosis and first relapse showed differences in seven of eight patients studied. In contrast, from first relapse onward, no clonal changes were observed in six patients studied. To investigate the occurrence of crosslineage TCR delta rearrangements in normal B and T cells, fluorescence-activated cell sorter-sorted peripheral blood CD19+/CD3- and CD19-/CD3+ cell populations from three healthy donors were analyzed. V delta 2D delta 3 rearrangements were detected at low frequencies in both B and T cells, which suggests that V delta 2-to-D delta 3 joining also occurs during normal B-cell differentiation. A model for crosslineage TCR delta rearrangements in B-precursor ALL is deduced that explains the observed clonal changes between diagnosis and relapse and is compatible with multistep leukemogenesis of B-precursor ALL. 相似文献
5.
6.
7.
8.
Sequence comparison of human and yeast telomeres identifies structurally distinct subtelomeric domains 总被引:6,自引:2,他引:6
Flint J; Bates GP; Clark K; Dorman A; Willingham D; Roe BA; Micklem G; Higgs DR; Louis EJ 《Human molecular genetics》1997,6(8):1305-1313
We have sequenced and compared DNA from the ends of three human
chromosomes: 4p, 16p and 22q. In all cases the pro-terminal regions are
subdivided by degenerate (TTAGGG)n repeats into distal and proximal sub-
domains with entirely different patterns of homology to other chromosome
ends. The distal regions contain numerous, short (<2 kb) segments of
interrupted homology to many other human telomeric regions. The proximal
regions show much longer (approximately 10-40 kb) uninterrupted homology to
a few chromosome ends. A comparison of all yeast subtelomeric regions
indicates that they too are subdivided by degenerate TTAGGG repeats into
distal and proximal sub-domains with similarly different patterns of
identity to other non-homologous chromosome ends. Sequence comparisons
indicate that the distal and proximal sub-domains do not interact with each
other and that they interact quite differently with the corresponding
regions on other, non- homologous, chromosomes. These findings suggest that
the degenerate TTAGGG repeats identify a previously unrecognized,
evolutionarily conserved boundary between remarkably different subtelomeric
domains.
相似文献
9.
da Cunha JP Nakayasu ES Elias MC Pimenta DC Téllez-Iñón MT Rojas F Muñoz MJ Manuel M Almeida IC Schenkman S 《Molecular and biochemical parasitology》2005,140(1):75-86
Histone H1 of most eukaryotes is phosphorylated during the cell cycle progression and seems to play a role in the regulation of chromatin structure, affecting replication and chromosome condensation. In trypanosomatids, histone H1 lacks the globular domain and is shorter when compared with the histone of other eukaryotes. We have previously shown that in Trypanosoma cruzi, the agent of Chagas' disease, histone H1 is phosphorylated and this increases its dissociation from chromatin. Here, we demonstrate using mass spectrometry analysis that T. cruzi histone H1 is only phosphorylated at the serine 12 in the sequence SPKK, a typical cyclin-dependent kinase site. We also found a correlation between the phosphorylation state of histone H1 and the cell cycle. Hydroxyurea and lactacystin, which, respectively, arrest parasites at the G1/S and G2/M stages of the cell cycle, increased the level of histone H1 phosphorylation. Cyclin-dependent kinase-related enzymes TzCRK3, and less intensely the TzCRK1 were able to phosphorylate histone H1 in vitro. Histone H1 dephosphorylation was prevented by treating the parasites with okadaic acid but not with calyculin A. These findings suggest that T. cruzi histone H1 phosphorylation is promoted by cyclin dependent kinases, present during S through G2 phase of the cell cycle, and its dephosphorylation is promoted by specific phosphatases. 相似文献
10.