How the ability of bacteria to inactivate natural antiinfectious resistance factors affects their resistance to the bactericidal action of the blood (Serum)

A relationship is found between the resistance ofEscherichia coli, Staphylococcus aureus, andStaphylococcus epidermidis to human serum and whole blood and their ability to inactivate the factors of natural antiinfectious resistance (lysozyme, complement, immunoglobulins, and a bactericidal fraction of leukocytic interferon). Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 121, N ^o 2, pp. 174–176, February, 1996     

Effects of microbial metabolites on catalase activity and growth of Staphylococcus aureus 6538 P

The effects of cell extracts and supernatants ofLactobacillus spp. andCorynebacterium spp. on catalase activity and growth ofStaphylococcus aureus 6538 P were studied. Intra- and extracellular metabolites of lactobacilli and corynebacteria inhibited catalase activity ofS. aureus 6538 P. The growth ofS. aureus 6538 P decreased after incubation with lactobacillus metabolites. The inhibitory effect of intra- and extracellular metabolites of lactobacilli and corynebacteria on catalase activity ofS. aureus is a possible pathway of microbial interrelations responsible for the formation and/or development of microbial biocenoses. Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 130, No. 7, pp. 80–82, July, 2000     

Prognosis of postinjection abscesses course with use of mathematical model]

Virulent and persistent characteristics of staphylococci isolated from 100 patients with various types of course of the abscessed forms of postinjection suppuration were defined. Comparative analysis of 11 biological characteristics of the microorganisms was carried out. An important role of the complex of properties of Staphylococcus aureus was established, including lysozyme, proteolytic, fibrinolytic activities, ability for inactivation of immunoglobulins through their Fc-receptors' binding to protein A, antilysozyme, "antiinterferon", anticomplement activities in determination of protracted character of festering and inframmatory process initiated by them. The model for prognosis of the course of postinjection abscesses was developed with the help of discriminant analysis, being based on the analysis of the informative properties of the pathogen.     

Dietary polyamines promote the growth of azoxymethane-induced aberrant crypt foci in rat colon

We have examined whether dietary polyamines influence the formation and initial growth of azoxymethane (AOM)-induced aberrant crypt foci (ACF) in rat colon. Effects of a combination of dietary polyamines at three dose levels (putrescine: 50, 280, 740 nmol/g; spermidine: 10, 261, 763 nmol/g; spermine: 1, 31, 91 nmol/g) in the polyamine-poor AIN-76A diet were studied in animals in two different experimental situations: animals treated with AOM alone and animals treated with AOM + difluoromethylornithine (DFMO), a specific inhibitor of endogenous polyamine synthesis. In both experimental situations, dietary polyamines enhanced the growth of ACF, expressed as the number of large ACF (foci with three or more aberrant crypts, ACF > or = 3), whereas the formation of ACF, expressed as the number of ACF, was apparently not altered. In animals treated with AOM alone, maximal growth enhancing effect on ACF was nearly obtained with the median level of dietary polyamine. In rats fed a low polyamine diet, basic AIN-76A, DFMO reduced the growth of AOM-induced ACF by 83%. This inhibitory effect of DFMO was counteracted by dietary polyamines in a dose- dependent manner, and it was abolished at the highest level of polyamines. In conclusion, it was demonstrated that dietary polyamines are able to enhance the growth of AOM-induced ACF. Further, dietary polyamines reversed the DFMO-caused inhibition of ACF growth, probably by compensating for the DFMO-reduced endogenous polyamine synthesis.      

New microbiological approach to analyzing the sanitary and ecological status of natural water reservoirs

The paper shows that in addition to the qualitative characteristics that are of rather informative value for evaluation of the sanitary and ecological conditions of environmental objects, there are suitable qualitative characteristics that may reveal the changes preceding irreversible changes in the natural environment. As such, the factors of persistence which are rather widely prevalent in aquatic microorganisms. By taking into account that the persistent properties of microorganisms label an ecological affiliation of bacteria, ecological type-specific differences of hydrobionts in persistent characteristics, they may be used as informative criteria for an indicator value in sanitary and ecological work. The value of these materials is great in the applied point of view as just now they open up vistas for microbiological monitoring and ecological studies of natural water reservoirs.     

Features of surgical infection of soft tissues

Microflora from surgical infection foci of soft tissues was studied in 92 patients with local, 78 patients with spread and 10 patients with generalized forms of pyo-inflammatory processes. Increase of bacterial contamination of surgical infection foci, more frequent detection of gram-negative bacteria, associations of microorganisms, high antibiotics resistance and persistent characteristics of bacteria in spread and generalized forms of surgical infections were found. Increase of persistent potential of infective agents is significant for prolongation of the disease and generalization of infectious process.     

Microbiological specifics in the course of suppurative inflammatory processes in soft tissues of patients with diabetes mellitus

The focal microflora of pyo-inflammatory processes has been studied in dynamics in 200 patients with diabetes mellitus. It was found that a longer and more severe course was due to the changed specific composition of causative agents and to the increased specific diversity of the latter in the process of pyo-inflammatory progressing. It was established that the usage of the method of "close" wound management with the application of oxytocin inhibited the change of the causative agents in the course of the disease, led to a more rapid elimination of microorganisms from the pyo-inflammatory focus, to the less frequency of recurrent surgical interventions and shorter duration of courses of treatment in contrast to the results obtained with the other methods of treatment.