Effect of catalin on radical production in aqueous solutions and cross-linking in deoxyribonucleoprotein complex

The effect of catalin on cross-linking in the DNA-protein system is studied after illumination with visible and UV light. Catalin is shown to stimulate this process. The use of a hydroxylamine trap reveals that catalin (10–100 μM) induces the accumulation of free radicals in aqueous solutions as soon as 10 minutes after irradiation. A considerable amount of O ₂ ^- is fixed in the medium during illumination of catalin aqueous solution. Superoxide dismutase added to the incubation medium considerably reduced the number of recorded radicals. Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 118, N ^o 8, pp. 142–144, August, 1994 Presented by I. P. Ashmarin, Member of the Russian Academy of Medical Sciences