全文获取类型
收费全文 | 1193篇 |
免费 | 73篇 |
国内免费 | 17篇 |
专业分类
耳鼻咽喉 | 31篇 |
儿科学 | 51篇 |
妇产科学 | 51篇 |
基础医学 | 239篇 |
口腔科学 | 37篇 |
临床医学 | 132篇 |
内科学 | 272篇 |
皮肤病学 | 20篇 |
神经病学 | 55篇 |
特种医学 | 60篇 |
外科学 | 128篇 |
综合类 | 11篇 |
一般理论 | 1篇 |
预防医学 | 84篇 |
眼科学 | 7篇 |
药学 | 42篇 |
中国医学 | 1篇 |
肿瘤学 | 61篇 |
出版年
2022年 | 9篇 |
2021年 | 13篇 |
2020年 | 12篇 |
2019年 | 13篇 |
2018年 | 20篇 |
2017年 | 10篇 |
2016年 | 19篇 |
2015年 | 35篇 |
2014年 | 33篇 |
2013年 | 47篇 |
2012年 | 66篇 |
2011年 | 49篇 |
2010年 | 43篇 |
2009年 | 61篇 |
2008年 | 52篇 |
2007年 | 64篇 |
2006年 | 55篇 |
2005年 | 38篇 |
2004年 | 47篇 |
2003年 | 54篇 |
2002年 | 36篇 |
2001年 | 30篇 |
2000年 | 40篇 |
1999年 | 30篇 |
1998年 | 47篇 |
1997年 | 33篇 |
1996年 | 22篇 |
1995年 | 19篇 |
1994年 | 24篇 |
1993年 | 9篇 |
1992年 | 22篇 |
1991年 | 19篇 |
1990年 | 13篇 |
1989年 | 25篇 |
1988年 | 18篇 |
1987年 | 20篇 |
1986年 | 22篇 |
1985年 | 15篇 |
1984年 | 15篇 |
1983年 | 8篇 |
1982年 | 12篇 |
1979年 | 8篇 |
1977年 | 4篇 |
1975年 | 5篇 |
1973年 | 3篇 |
1971年 | 5篇 |
1970年 | 5篇 |
1969年 | 3篇 |
1968年 | 3篇 |
1966年 | 5篇 |
排序方式: 共有1283条查询结果,搜索用时 15 毫秒
1.
SH Lee† CP Choi‡ HC Eun† OS Kwon† 《Journal of the European Academy of Dermatology and Venereology》2006,20(7):860-863
BACKGROUND: On December 26, 2004, the biggest earthquake for 40 years, measuring 9.0 on the Richter scale, triggered a tsunami that pounded the coastal areas of South Asia and East Africa. The effects of the tsunami on skin conditions have not been evaluated. OBJECTIVE: To determine the influence of the tsunami on skin conditions by evaluating the skin problems of patients presenting at hospitals after the tsunami. METHODS: Between 5 and 25 January 2005, two dermatologists evaluated patients who complained of skin problems at an outpatient clinic and emergency room of a general hospital in Banda Aceh, Aceh Province, Indonesia. RESULTS: The total number of patients that presented during the study period was 235 (131 males and 104 females), and they had a total of 265 skin problems. In terms of age distribution, most subjects were in their fourth decade (23.0%), followed by the third (22.6%) and fifth decade (16.6%). The most prevalent skin problems were infections-infestations (32.5%), followed by eczemas (29.8%) and traumatic skin disorders (29.4%). In males, traumatic skin disorders were most common. The great majority of infection-infestation cases involved superficial fungal infections. Contact dermatitis accounted for three-quarters of eczema cases, and mainly involved the arms (40.0%) and legs (27.1%). The majority of traumatic skin disorders were lacerations, punctures and penetrations, and the feet (44.7%) and hands (18.8%) were most frequently affected. CONCLUSIONS: Unhygienic conditions, exposure to a hazardous environment and contact with various objects during and after the tsunami probably increased the prevalence of infections-infestations, traumatic skin disorders and contact dermatitis. To prevent these problems and associated secondary bacterial infections, health-related education and early medical management are required. 相似文献
2.
Eva Maria Valesky Diamant Thai Markus Meissner Christian Beier Manfred Wolter Helmut Schfer Roland Kaufmann 《Journal der Deutschen Dermatologischen Gesellschaft》2007,5(9):770-772
With 1–1.5 million cases reported every year cutaneous leishmaniasis represents an increasing health problem. The course of cutaneous leishmaniasis varies from a single self‐healing ulcer to a persistent ulcer or progressive mucosal disease with nasopharyngeal destruction. An enormous array of topical and systemic treatment modalities has been endorsed. The response to treatment depends on the species of parasite as well as the host's immunological and genetic status. Species‐specific treatment guidelines based on evidence from controlled studies are highly desirable. We present two cases of cutaneous leishmaniasis, one in a child and one during pregnancy, reviewing various diagnostic and therapeutic measures with special attention to problems in young and pregnant patients. 相似文献
3.
4.
5.
Arend Bökenkamp Bettina Bohnhorst Christian Beier Norbert Albers Gisela Offner Johannes Brodehl 《Pediatric nephrology (Berlin, Germany)》1994,8(2):181-185
Gingival hyperplasia is a common side-effect of immunosuppression with cyclosporine A. Nifedipine is often used to control hypertension in kidney graft recipients. Analysis of gingival status in 106 children transplanted at our centre, and treated either with azathioprine, cyclosporine A or both, revealed significantly higher degrees of gingival overgrowth in those children receiving a combination of cyclosporine A and nifedipine compared with those children treated with cyclosporine A or nifedipine alone. Seven children undergoing gingivectomy at our centre over the past few years had received this combination. After a change in the antihypertensive regimen, avoiding long-term nifedipine medication, and improved dental care with chlorhexidine gel, we noted a reduction in the degree of gingival hyperplasia. In the majority of patients, nifedipine could be replaced by a single drug, usually hydralazine. We therefore recommend avoiding calcium channel blockers in the long-term management of hypertension in patients receiving cyclosporine. 相似文献
6.
Comment on Pfäfflin’s (2008) “Good Enough to Eat” 总被引:1,自引:1,他引:0
Klaus M. Beier 《Archives of sexual behavior》2009,38(2):164-165
7.
Construction and characterization of Listeria monocytogenes mutants with in-frame deletions in the response regulator genes identified in the genome sequence
下载免费PDF全文
![点击此处可从《Infection and immunity》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Two-component systems are widely distributed in prokaryotes where they control gene expression in response to diverse stimuli. To study the role of the sixteen putative two-component systems of Listeria monocytogenes systematically, in frame deletions were introduced into 15 out of the 16 response regulator genes and the resulting mutants were characterized. With one exception the deletion of the individual response regulator genes has only minor effects on in vitro and in vivo growth of the bacteria. The mutant carrying a deletion in the ortholog of the Bacillus subtilis response regulator gene degU showed a clearly reduced virulence in mice, indicating that DegU is involved in the regulation of virulence-associated genes. 相似文献
8.
IMPT1, an imprinted gene similar to polyspecific transporter and multi- drug resistance genes 总被引:5,自引:1,他引:5
Dao D; Frank D; Qian N; O'Keefe D; Vosatka RJ; Walsh CP; Tycko B 《Human molecular genetics》1998,7(4):597-608
Human chromosome 11p15.5 and distal mouse chromosome 7 include a
megabase-scale chromosomal domain with multiple genes subject to parental
imprinting. Here we describe mouse and human versions of a novel imprinted
gene, IMPT1 , which lies between IPL and p57 KIP2 and which encodes a
predicted multi-membrane-spanning protein similar to bacterial and
eukaryotic polyspecific metabolite transporter and multi- drug resistance
pumps. Mouse Impt1 and human IMPT1 mRNAs are highly expressed in tissues
with metabolite transport functions, including liver, kidney, intestine,
extra-embryonic membranes and placenta, and there is strongly preferential
expression of the maternal allele in various mouse tissues at fetal stages.
In post-natal tissues there is persistent expression, but the allelic bias
attenuates. An allelic expression bias is also observed in human fetal and
post-natal tissues, but there is significant interindividual variation and
rare somatic allele switching. The fact that Impt1 is relatively repressed
on the paternal allele, together with data from other imprinted genes,
allows a statistical conclusion that the primary effect of human chromosome
11p15.5/mouse distal chromosome 7 imprinting is domain-wide relative
repression of genes on the paternal homolog. Dosage regulation of the
metabolite transporter gene(s) by imprinting might regulate placental and
fetal growth.
相似文献
9.
Foy BD Magalhaes T Injera WE Sutherland I Devenport M Thanawastien A Ripley D Cárdenas-Freytag L Beier JC 《Infection and immunity》2003,71(4):2032-2040
Vaccines that induce mosquito-killing (mosquitocidal) activity could substantially reduce the transmission of certain mosquito-borne diseases, especially vaccines against African malaria vectors, such as the mosquito Anopheles gambiae. To generate and characterize antimosquito immunity we immunized groups of mice with two individual A. gambiae midgut cDNAs, Ag-Aper1 (a secreted peritrophic matrix protein) and AgMuc1 (a midgut-bound mucin), and an A. gambiae midgut cDNA library from blood-fed mosquitoes. We observed significantly increased mortality among mosquitoes that fed on either the AgMuc1- or the cDNA library-immunized mice compared to that of controls, but no differences were observed among those fed on Ag-Aper1-immunized mice. Analysis of the humoral and cellular immune responses from mice showed that the induced mosquitocidal effect was associated with immune profiles characterized by elevated tumor necrosis factor alpha and gamma interferon cytokine levels and very low antibody titers. Furthermore, an additional immunization of cDNA library-immunized mice with midgut protein shifted immunity toward a Th2-type immune response, characterized by elevated antibody titers and high interleukin-5 and interleukin-10 cytokine levels; importantly, mosquitoes feeding on these mice exhibited no undue mortality. Finally, when immune sera was ingested by mosquitoes through a membrane feeder, no effect on mosquito mortality was observed, indicating that serum factors alone were not responsible for the mosquitocidal effect. Our results demonstrate that mosquitocidal immunity in mice can be consistently generated by midgut cDNA immunization and suggest this cDNA-induced mosquitocidal immunity is cell mediated. 相似文献
10.