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Tetsuya Tanaka Suguru Kawano Sumihiro Nakao Rika Umemiya-Shirafuji Md. Morshedur Rahman Damdinsuren Boldbaatar Banzragch Battur Min Liao Kozo Fujisaki 《Ticks and Tick》2010,1(4):178-185
A full-length cDNA-encoding lysozyme was obtained from cDNA libraries of salivary glands of the hard tick Haemaphysalis longicornis and designated as HlLysozyme. The HlLysozyme sequence represents an open reading frame for a putative signal peptide and the mature protein composed of 121 amino acids. The calculated molecular weight of the protein is 13.7 kDa, and the theoretical isoelectric point is 9.85. HlLysozyme shares 41–79% amino acid sequence identity with the lysozymes of other organisms. The activity of recombinant HlLysozyme expressed in Escherichia coli was confirmed by a lytic zone assay using lyophilized Micrococcus lysodeikticus. The HlLysozyme activity decreased at 70 °C and was demonstrated at acidic side and neutral in a pH range. Elevated gene expression of HlLysozyme was observed when female ticks were challenged with bacteria, suggesting possible roles of lysozyme as an innate immunity of ticks against microorganisms. 相似文献
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Mizushima Daiki Amgalanbaatar Tovuu Davaasuren Batdorj Molefe Nthatisi Innocentia Battur Banzragch Battsetseg Badgar Inoue Noboru Yokoyama Naoaki Suganuma Keisuke 《Parasitology research》2018,117(9):2913-2919
Parasitology Research - Our previous studies report epidemics of non-tsetse-transmitted equine trypanosomosis in Mongolia. However, the current status of non-tsetse-transmitted equine... 相似文献
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Guohong Zhang Xiaohong Huang Damdinsuren Boldbaatar Banzragch Battur Badgar Battsetseg Houshuang Zhang Longzheng Yu Yan Li Yuzi Luo Shinuo Cao Youn-Kyong Goo Junya Yamagishi Jinlin Zhou Shoufa Zhang Hiroshi Suzuki Ikuo Igarashi Takeshi Mikami Yoshifumi Nishikawa Xuenan Xuan 《Vaccine》2010
Toxoplasma gondii and Neospora caninum are closely related apicomplexan parasites. The surface antigen 1 of T. gondii (TgSAG1) is a major immunodominant antigen and, therefore, is considered to be a good candidate for the development of an effective recombinant vaccine against toxoplasmosis. In this study, N. caninum stably expressing the TgSAG1 gene (Nc/TgSAG1) was constructed using pyrimethamine-resistant DHFR-TS and GFP genes as double-selection markers. The expression level, molecular weight, and antigenic property of recombinant TgSAG1 expressed by the Nc/TgSAG1 were similar to those of the native TgSAG1. The mice immunized with Nc/TgSAG1 induced TgSAG1-specific Th1-dominant immune responses and protected the mice from a lethal challenge infection with T. gondii. These results indicate that N. caninum may provide a new tool for the production of a live recombinant vector vaccine against toxoplasmosis in animals. To our knowledge, this is the first report to evaluate the usefulness of N. caninum-based live vaccine. 相似文献
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Bayasgalan Mungun-Ochir Noriyuki Horiuchi Adilbish Altanchimeg Kenji Koyama Keisuke Suganuma Uranbileg Nyamdolgor Ken-ichi Watanabe Purevdorj Baatarjargal Daiki Mizushima Banzragch Battur Naoaki Yokoyama Badgar Battsetseg Noboru Inoue Yoshiyasu Kobayashi 《Neuromuscular disorders : NMD》2019,29(6):437-443
Dourine is an equine protozoan disease caused by Trypanosoma equiperdum. Dourine-afflicted animals die after developing neurological clinical signs, such as unilateral paresis. The disease has been a problem for many years; however, the pathogenesis regarding the neurological clinical signs of dourine has been unclear. In the present study, we conducted a histopathological examination in order to investigate the mechanisms by which dourine-afflicted horses develop the accompanying neurological clinical signs. Four dourine-afflicted horses in Mongolia were evaluated. An apparently healthy horse exhibited multifocal neuritis without axonal or myelin degeneration. The other horses, which had obvious neurological clinical signs, also exhibited multifocal neuritis. In particular, the nerves that innervated areas associated with neurological clinical signs exhibited neuritis with demyelination in the latter horses. Inflamed, non-demyelinating nerves were infiltrated with B lymphocytes and T lymphocytes; while inflamed, demyelinating nerves were infiltrated with mononuclear phagocytes. Our observations revealed lesion progression in the nerves, such that polyradiculoneuropathy could explain the accompanying neurological clinical signs of dourine. To our knowledge, this is the first report to describe a pathogenic mechanism for the development of the neurological clinical signs found in dourine-afflicted horses. 相似文献
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