全文获取类型
收费全文 | 77篇 |
免费 | 11篇 |
专业分类
儿科学 | 3篇 |
基础医学 | 21篇 |
口腔科学 | 2篇 |
临床医学 | 3篇 |
内科学 | 11篇 |
皮肤病学 | 2篇 |
神经病学 | 5篇 |
特种医学 | 2篇 |
外科学 | 24篇 |
预防医学 | 4篇 |
药学 | 10篇 |
中国医学 | 1篇 |
出版年
2022年 | 1篇 |
2021年 | 1篇 |
2020年 | 1篇 |
2019年 | 3篇 |
2018年 | 3篇 |
2017年 | 1篇 |
2016年 | 3篇 |
2015年 | 4篇 |
2014年 | 2篇 |
2013年 | 5篇 |
2012年 | 4篇 |
2011年 | 7篇 |
2009年 | 1篇 |
2008年 | 2篇 |
2007年 | 6篇 |
2006年 | 4篇 |
2005年 | 1篇 |
2003年 | 1篇 |
2002年 | 2篇 |
2001年 | 5篇 |
2000年 | 4篇 |
1998年 | 1篇 |
1997年 | 1篇 |
1996年 | 1篇 |
1995年 | 2篇 |
1994年 | 3篇 |
1993年 | 2篇 |
1992年 | 1篇 |
1990年 | 1篇 |
1989年 | 9篇 |
1988年 | 3篇 |
1987年 | 1篇 |
1985年 | 1篇 |
1974年 | 1篇 |
排序方式: 共有88条查询结果,搜索用时 31 毫秒
1.
BACKGROUND: Rat basophilic leukemia (RBL-2H3) cells are well characterized in terms of morphological and biochemical changes upon activation, and have been extensively used as a model system for studying the mechanisms of the immediate hypersensitivity reaction. To investigate whether overexpression of heat shock/stress proteins (HSP) is involved in the mast cell-dependent reactivity, we examined the adaptive responses of RBL-2H3 cells to classical stress conditions such as heat shock or oxidative injury produced by an aqueous extract of tobacco smoke. METHODS: HSP were determined by flow cytometry and immunocytochemistry. Degranulation was confirmed as the release of beta-hexosaminidase, determined spectrophotometrically, and by electron microscopy experiments. RESULTS: We found that RBL-2H3 cells respond to heat shock or oxidative injury by the synthesis of both the inducible 72 kDa HSP (Hsp70), and the oxidation-specific 32 kDa heme oxygenase (HO)-1. Heat shock induced mainly Hsp70 in a cell growth-dependent manner, whereas oxidative stress induced mainly HO-1 in a cell growth-independent manner. However, heat shock or oxidative stress had no significant effects on degranulation. CONCLUSION: Stress-mediated synthesis of HSP was not associated with RBL-2H3 degranulation and likewise, degranulation did not induce HSP. 相似文献
2.
Characterization of murine lung inflammation after infection with parental Bordetella pertussis and mutants deficient in adhesins or toxins. 总被引:2,自引:2,他引:2
下载免费PDF全文
![点击此处可从《Infection and immunity》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Bordetella pertussis expresses factors such as filamentous hemagglutinin, agglutinogens, pertactin, and pertussis toxin, which participate in bacterial adhesion; pertussis toxin, dermonecrotic toxin, lipopolysaccharide, and tracheal cytotoxin, which are responsible for toxic effects; and adenylate cyclase-hemolysin, which is required to initiate infection. By using a murine respiratory model, we showed that the RGD sequences of filamentous hemagglutinin and pertactin are important for bacterial persistence. However, mutants deficient in filamentous hemagglutinin and agglutinogens or in pertactin and the RGD sequence of filamentous hemagglutinin behaved as did wild-type B. pertussis, i.e., induced bronchopneumonia, alveolitis, and an influx of macrophages, lymphocytes, and polymorphonuclear leukocytes into bronchoalveolar lavage fluids. These results suggest that these adhesins are not involved in the induction of pulmonary lesions following infection. The intensity of inflammation was markedly reduced after infection with mutants deficient in either hemolytic activity or pertussis toxin expression, whereas a mutant devoid of adenylate cyclase activity behaved as did the avirulent mutant. Pertussis toxin and adenylate cyclase-hemolysin may act indirectly by altering immune cell functions and thus allowing other factors, such as filamentous hemagglutinin, agglutinogens, and pertactin, to trigger adhesion and lipopolysaccharide, dermonecrotic toxin, and tracheal cytotoxin to induce their toxic effects. However, it is possible that pertussis toxin is also responsible for the induction of some pulmonary alterations. 相似文献
3.
Hannah Kaminski Isabelle Garrigue Lionel Couzi Benjamin Taton Thomas Bachelet Jean-Fran?ois Moreau Julie Déchanet-Merville Rodolphe Thiébaut Pierre Merville 《Journal of the American Society of Nephrology : JASN》2016,27(2):637-645
Cytomegalovirus (CMV) infection in solid-organ transplantation is associated with increased morbidity and mortality, particularly if a CMV mutant strain with antiviral resistance emerges. Monitoring CMV–specific T cell response could provide relevant information for patient care. We and others have shown the involvement of Vδ2neg
γδ T cells in controlling CMV infection. Here, we assessed if Vδ2neg
γδ T cell kinetics in peripheral blood predict CMV infection resolution and emergence of a mutant strain in high–risk recipients of kidney transplants, including 168 seronegative recipients receiving organs from seropositive donors (D+R−) and 104 seropositive recipients receiving antithymocyte globulins (R+/ATG). Vδ2neg
γδ T cell percentages were serially determined in patients grafted between 2003 and 2011. The growing phase of Vδ2neg
γδ T cells was monitored in each infected patient, and the expansion rate during this phase was estimated individually by a linear mixed model. A Vδ2neg
γδ T cell expansion rate of ˃0.06% per day predicted the growing phase. The time after infection at which an expansion rate of 0.06% per day occurred was correlated with the resolution of CMV DNAemia (r=0.91; P<0.001). At 49 days of antiviral treatment, Vδ2neg
γδ T cell expansion onset was associated with recovery, whereas absence of expansion was associated with recurrent disease and DNAemia. The appearance of antiviral–resistant mutant CMV strains was associated with delayed Vδ2neg
γδ T cell expansion (P<0.001). In conclusion, longitudinal surveillance of Vδ2neg
γδ T cells in recipients of kidney transplants may predict CMV infection resolution and antiviral drug resistance. 相似文献
4.
5.
Rheological Changes After Stenting of a Cerebral Aneurysm: A Finite Element Modeling Approach 总被引:1,自引:0,他引:1
Ohta M Wetzel SG Dantan P Bachelet C Lovblad KO Yilmaz H Flaud P Rüfenacht DA 《Cardiovascular and interventional radiology》2005,28(6):768-772
Hemodynamic changes in intracranial aneurysms after stent placement include the appearance of areas with stagnant flow and
low shear rates. We investigated the influence of stent placement on blood flow velocity and wall shear stress of an intracranial
aneurysm using a finite element modeling approach. To assess viscosity changes induced by stent placement, the rheology of
blood as non-Newtonian fluid was taken into account in this model. A two-dimensional model with a parent artery, a smaller
branching artery, and an aneurysm located at the bifurcation, before and after stent placement, was used for simulation. Flow
velocity plots and wall shear stress before and after stent placement was calculated over the entire cardiac circle. Values
for dynamic viscosity were calculated with a constitutive equation that was based on experimental studies and yielded a viscosity,
which decreases as the shear rate increases. Stent placement lowered peak velocities in the main vortex of the aneurysm by
a factor of at least 4 compared to peak velocities in the main artery, and it considerably decreased the wall shear stress
of the aneurysm. Dynamic viscosity increases after stent placement persisted over a major part of the cardiac cycle, with
a factor of up to 10, most pronounced near the dome of the aneurysm. Finite element modeling can offer insight into rheological
changes induced by stent treatment of aneurysms and allows visualizing dynamic viscosity changes induced by stent placement. 相似文献
6.
7.
8.
Grenier N Poulain S Lepreux S Gennisson JL Dallaudière B Lebras Y Bavu E Servais A Meas-Yedid V Piccoli M Bachelet T Tanter M Merville P Couzi L 《European radiology》2012,22(10):2138-2146
Purpose
To evaluate the reliability of quantitative ultrasonic measurement of renal allograft elasticity using supersonic shear imaging (SSI) and its relationship with parenchymal pathological changes.Materials and methods
Forty-three kidney transplant recipients (22 women, 21 men) (mean age, 51?years; age range, 18–70?years) underwent SSI elastography, followed by biopsy. The quantitative measurements of cortical elasticity were performed by two radiologists and expressed in terms of Young’s modulus (kPa). Intra- and inter-observer reproducibility was assessed (Kruskal-Wallis test and Bland-Altman analysis), as well as the correlation between elasticity values and clinical, biological and pathological data (semi-quantitative Banff scoring). Interstitial fibrosis was evaluated semi-quantitatively by the Banff score and measured by quantitative image analysis.Results
Intra- and inter-observer variation coefficients of cortical elasticity were 20?% and 12?%, respectively. Renal cortical stiffness did not correlate with any clinical parameters, any single semi-quantitative Banff score or the level of interstitial fibrosis; however, a significant correlation was observed between cortical stiffness and the total Banff scores of chronic lesions and of all elementary lesions (R?=?0.34, P?=?0.05 and R?=?0.41, P?=?0.03,respectively).Conclusion
Quantitative measurement of renal cortical stiffness using SSI is a promising non-invasive tool to evaluate global histological deterioration.Key Points
? Supersonic shear imaging elastography can measure cortical stiffness in renal transplants ? The level of cortical stiffness is correlated with the global degree of tissue lesions ? The global histological deterioration of transplanted kidneys can be quantified using elastography 相似文献9.
George Thomas U. J. V. Castelo Branco J. M. Barbosa Filho M. Bachelet B. B. Vargaftig 《The Journal of pharmacy and pharmacology》1994,46(2):103-107
Abstract— The mechanism of action of a nonspecific smooth muscle relaxant, (O-methyl-)-N-(2,6-dihydroxybenzoyl)tyramine (riparin), a constituent of Aniba riparia (Nees) Mez. (Lauraceae) was studied in relation to Ca2+ metabolism in smooth muscle tissues and in guinea-pig alveolar leucocytes. In rat depolarized uterus, riparin inhibited in a reversible and noncompetitive manner CaCl2-induced contraction, a response mediated through voltage-dependent Ca2+ channels. The pD2 value (mean±s.e.m.) for riparin was 4·98±006. When compared with sodium nitroprusside (IC50 2·5 μm ), an antagonist of receptor-operated Ca2+ channels, riparin was ineffective in suppressing noradrenaline-induced sustained contractions of rabbit aortic strips. However, in the aorta, the compound inhibited intracellular calcium-dependent transient contractions of noradrenaline and riparin (IC50 10·1 μm ), was approximately two and a half times more potent than procaine (IC50 25·5 μm ), a known inhibitor. In guinea-pig alveolar leucocytes, riparin (IC50 3·2 μm ), inhibited intracellular Ca2+ accumulation induced by the calcium ionophore A23187. The results suggest that the inhibition of Ca2+ influx and of Ca2+ release from intracellular stores contribute to the spasmolytic effects of riparin, which may not involve cyclic AMP generation as the levels of this nucleotide were not increased in alveolar macrophages treated with riparin (10–100 μm ). 相似文献
10.
Al-Laith M. Weyer A. Havet N. Dumarey C. Vargaftig B. Bachelet M. 《Inflammation research》1992,36(2):C287-C289
In the present study, we performed experiments to explore the role of IgG immunoglobulins in type I hypersensitivity reactions in the guinea-pig. Our results suggest that IgG immunoglobulins may be involved in allergic reactions, both through histamine release from mast cells and thromboxane A2 release from macrophages after challenge of passively sensitized cells.
相似文献