Neuron-specific enolase. Assessment by ELISA in patients with small cell carcinoma of the lung

Measurement of tissue-specific enolase isoenzymes may be of assistance in identifying small cell carcinomas of the lung and in distinguishing them from other pulmonary tumors. Enolase (E.C. 4.2.1.11) is a dimeric enzyme composed of various permutations of three immunologically distinct subunits alpha, beta, and gamma. Five isoenzymes alpha alpha, beta beta, gamma gamma, alpha beta, and alpha gamma have been identified. Immunohistochemical studies using antibodies to the gamma subunit have localized alpha gamma and gamma gamma specifically within neuronal and neuroendocrine tissues. Because of this limited distribution, neuron-specific enolase (NSE) can function as a biochemical marker for neuroendocrine tumors. The authors developed an enzyme-linked immunosorbent assay (ELISA) using the double antibody sandwich method. The sandwich is composed of rabbit antirat enolase that cross-reacts to the human gamma monomer, making the test specific for the gamma gamma isoenzyme. The avidin-biotin-peroxidase complex system is used to provide increased assay sensitivity. Serum samples from patients with histologically diagnosed small cell carcinoma have concentration of NSE 20- to 30-fold greater than that found in normal serum. Studies were conducted on patients with a variety of malignant pulmonary lesions and compared with controls to determine the value of NSE as a tumor marker.     

A Simplified Method for Measuring the Thickness of Glomerular Basement Membranes

Measurement of the thickness of glomerular basement membranes is required for the diagnosis of thin membrane nephropathy. Over the years various morphometric methods have been used but some are laborious so there is a need for establishing a simplified method for measuring thickness. In the present study 20 renal biopsies were used to carry out a comparative morphometric analysis between 2 methods. The first method was based on measuring thickness at the maximum number of available points, whereas for the second method, thickness was measured at only 5 points per loop. Since both methods gave mean values that are not statistically different in each patient, the authors recommend that the simplified method be used routinely for diagnosis.     

Evaluation of a brief psychological treatment for emotional disorders in primary care.

A randomized trial in general practice compared: (i) a brief psychological treatment (problem-solving) given by a psychiatrist; (ii) any treatment of the GP's choice, whether psychological or pharmacological. The patients had recent onset emotional disorders of poor prognosis. Patients in the problem-solving group showed significantly greater reductions in symptoms. Problem-solving as given by a psychiatrist was feasible in primary care and acceptable to patients. Problem-solving is now being evaluated as given by general practitioners trained in the method.     

The Relationship Between Cardiovascular Responses in the Laboratory and in the Field

The psychophysiological responses to laboratory stressors are often examined because it is believed that such responses relate to responsiveness in real life situations. This belief has seldom been tested. The changes in heart rate, pulse transit time, and respiration rate produced by a variety of laboratory tasks (active and passive coping and physical exercise) were related to ambulatory measures of heart rate in 32 young men. The field measures were the difference in heart rate between the waking day and when asleep, and estimates of the variability of heart rate during the day, derived from time series analyses. Average changes in heart rate and pulse transit time during specific tasks did not relate consistently to heart rate in the field. However, an active coping index, derived from the ratio of the peak heart rate during an active coping task to the peak during physical exercise related to all the field measures of heart rate responsiveness. This index, which may relate to measures of additional heart rate and heightened sympathetic response to stress, also correlated positively with Trait Anxiety and elevated basal sympathetic arousal, as measured by skin conductance level. Measures of the cardiovascular response to a passive coping task, the cold pressor, and exercise did not relate to heart rate responses in the field. The findings suggest that heightened cardiac responsiveness in real life is exhibited by subjects who show elevated peak responses to active coping stressors specifically.     

A Simple Activity Measure for Use with Ambulatory Subjects

A simple activity measure based on the EMG recorded from the thigh was developed to monitor the physical activity of ambulatory subjects. The performance of this activity measure was assessed during four major physical activities: running, walking, standing, and sitting. Heart rate was also monitored. The results were analysed within individuals using time series analysis. The heart rate and activity series (of consecutive 30-s means) required similar models within individuals, and both measures differentiated among running, walking, and non-movement. The activity measure did not discriminate between postures. This measure covaried closely with heart rate and emerged as a necessary and relatively more significant predictor of heart rate variance than knowledge of the type of physical activity the subjects were undertaking. Posture, however, did add a significant and independent contribution to heart rate variance. This activity measure appears to be a simple, reliable, and valuable method of measurement of physical activity variations in ambulatory subjects.     

Selective information processing in bulimia nervosa

The Stroop color-naming paradigm was used to investigate information processing in bulimia nervosa. Patients with bulimia nervosa were compared with agematched female controls as well as with a sample of men. It was found that the patients were slower than female controls were in color-naming words related to eating, shape, and weight, whereas there were no differences between the male and female controls. The findings suggest that bulimia nervosa is associated with the selective processing of information related to eating, shape, and weight and that this phenomenon may be restricted to those with an eating disorder of clinical severity.