CBS、MET、AMO三联疗法治疗HP阳性慢性胃病的疗效观察

已知幽门螺杆菌(HP)与胃炎、消化性绩疡和胃癌发生有密切关系，所以临床上清菌治疗有很重要的意义。我们采用胶态果胶铋(CBS)、甲硝唑(MET)和羟氨苄青霉素(AMO)治疗了一组HP感染的慢性胃病患，观察清菌率，取得良好效果。现报告如下。     

小鼠脑细胞内游离钙浓度与高血黏度致脑血管病的相关性

目的:探讨高血黏度致脑损伤的分子机制。方法:采用尾静脉注射100g/L高分子葡萄糖酐法建立小鼠高血黏度动物模型,以Fluo-3/AM为细胞内游离钙离子的荧光指示剂,用激光扫描共聚焦显微镜测定脑细胞内Ca2+i的变化。结果:高血黏度组小鼠脑细胞的荧光强度明显高于对照组,高血黏度组小鼠脑细胞的平均荧光强度为115.96±37.32,而对照组小鼠脑细胞的平均荧光强度为41.49±10.89,两者的差异有非常显著性意义(P<0.001)。说明高血黏度组小鼠脑细胞内的游离Ca2+浓度明显高于正常组脑细胞内的游离Ca2+浓度。相关分析显示脑细胞内的游离Ca2+浓度与全血黏度有显著相关性(rηH=0.769,rηL=0.821,P<0.001)。结论:高血黏度致脑细胞内钙超载是其致脑损伤的分子机制之一。     

主讲教师责任制教学模式探讨

通过对比分析主讲教师责任制教学模式与分配式教学模式下的学生学习成绩。结果发现前者显著地高于后者。因此应大力实施主讲教师责任制以密切师生关系，增强教师责任感，从而提高课堂教学质量及学生学习成绩。     

小鼠脑细胞内游离钙浓度与高血黏度致脑血管病的相关性

目的：探讨高血黏度致脑损伤的分子机制。方法：采用尾静脉注射100g／L高分子葡萄糖酐法建立小鼠高血黏度动物模型，以Fluo-3／AM为细胞内游离钙离子的荧光指示剂，用激光扫描共聚焦显微镜测定脑细胞内[Ca^2+]i的变化。结果：高血黏度组小鼠脑细胞的荧光强度明显高于对照组，高血黏度组小鼠脑细胞的平均荧光强度为115．96&;#177;37．32，而对照组小鼠脑细胞的平均荧光强度为41．49&;#177;10．89，两者的差异有非常显著性意义(P&;lt;0．001)。说明高血黏度组小鼠脑细胞内的游离Ca^2+浓度明显高于正常组脑细胞内的游离Ca^2+浓度。相关分析显示脑细胞内的游离Ca^2+浓度与全血黏度有显著相关性(r^ηH=O．769，r^ηL=0．821，P&;lt;0．001)。结论：高血黏度致脑细胞内钙超载是其致脑损伤的分子机制之一。     

VP16与细胞凋亡

鬼臼乙叉甙（Ｅｔｏｐｏｓｉｄｅ，ＶＰ１６）是人工半合成的鬼臼毒素类细胞毒药物，于１９７１年开始临床使用，１９８３年获得美国ＦＤＡ批准［１，２］。二十多年的临床应用表现：ＶＰ１６对许多实体瘤及造血系统恶性肿瘤有明显疗效［３，４］，已成为临床上主要的化疗...     

中央型肺癌侵犯周围大血管CT评价

目的 评价螺旋CT增强扫描、后处理重建及CT血管成像判断中央型肺癌侵犯周围大血管的准确性.方法 对15例中央型肺癌患者行螺旋CT增强扫描,对比增强横断面CT图像、后处理重建及CT血管成像的图像,分析中央型肺癌侵犯邻近血管的影像学特征.结果 15例中央型肺癌患者增强CT检查图像及重建图像显示均有不同程度侵犯邻近肺动脉和肺静脉,图像上均可见脂肪间隙消失、血管明显狭窄闭塞的影像特征.结论 CT增强扫描可提高对肿瘤侵犯周围大血管判断的敏感度及准确率,同时对肺癌进行临床分期及术前评估有重要意义.     

地塞米松复合聚己内酯胶原支架材料的构建及性能评价

BACKGROUND: Tissue engineering scaffold materials have been widely used in all kinds of tissue and nerve repair, but there are many limitations and the effect is not good. OBJECTIVE: To construct a kind of tissue engineering scaffold material for the regeneration and repair of spinal cord injury. METHODS: The dexamethasonemicrospheres were prepared by emulsification-solvent evaporation. The comprehensive scores of encapsulation efficiency, drug-loading rate and yield were taken as the indexes. The effect of dosage of dexamethasone and polylactic acid-glycolic acid copolymer and mass fraction of polyvinyl alcohol on formulation process of dexamethasone sustained-release microsphere was inspected by orthogonal experiment. The characterization of microspheres was observed by scanning electron microscope. The nanofiber scaffold of compound dexamethasone microspheres was prepared by taking collagen protein and polycaprolactone as raw materials using electrospinning technology. The mouse bone marrow mesenchymal stem cells were co-cultured with the scaffold for 3 days. Cell morphology was observed by scanning electron microscope. Composite material was implanted into the defect of spinal cord in rats. RESULTS AND CONCLUSION: The optimal preparation process of dexamethasone sustained-release microspheres: dosage of dexamethasone was 10 mg, dosage of poly lactic acid-glycolic acid copolymer was 80%, mass fraction of polyvinyl alcohol was 0.5%. Appearance of dexamethasone microspheres was smooth, with a round surface. The encapsulation efficiency, drug-loading rate and yield of microspheres were (2.26±0.03)%, (83.62±0.21)% and (90.87±2.45)% respectively. The growth of mouse bone marrow mesenchymal stem cells was good on the surface of compound dexamethasone microspheres. There was no immunological reaction between the implant material and host, and the material was degraded gradually with time. These results demonstrate that the compound dexamethasone microsphere scaffold has good biocompatibility, which is a favorable kind of biological scaffold material.        

海拔对不同生活环境藏、汉两族血压、血糖、血尿酸和血脂水平的影响

目的 探讨海拔时不同生活环境藏、汉族血压、血糖、血尿酸和血脂水平的影响,为不同海拔地区高血压、高血糖、高尿酸血症和高脂血症的预防提供研究资料.方法 275例被调查者为世居或长期生活在海拔3 500 m及2 400 m的牧区藏族、城镇藏族、城镇汉族人群,测定被调查者收缩压、舒张压、血糖、血尿酸、血脂,时检测结果进行比较.结果 3 500 m海拔中,城镇汉族收缩压高于城镇藏族收缩压,(121.83±18.22)mmHg vs(111.92±12.97)mmHg(P<0.05),牧区藏族血糖高于城镇藏族血糖,(6.07±2.29)mmol/L vs(4.58±1.13)mmol/L(P<0.01),城镇汉族甘油三酯(TG)高于牧区藏族TG,(1.52±0.94)mmol/L vs(1.07±0.64)mmol/L(P<0.05),牧区藏族低密度脂蛋白胆固醇(LDL-C)高于城镇汉族LDL-C,(2.58±0.75)mmol/L vs(2.13±0.94)mmol/L(P<0.05),但牧区藏族、城镇藏族、城镇汉族之间舒张压、血尿酸、总胆固醇(TC)、高密度脂蛋白胆固醇(HDL-C)差异无统计学意义(P>0.05).2 400 m海拔中,牧区藏族舒张压高于城镇藏族舒张压,(86.14±9.84)mmHg vs(80.17±10.98)mmHg(P<0.05),城镇藏族HDL-C高于城镇汉族HDL-C,(1.73±0.45)mmol/L vs(1.49±0.27)mmol/L(P<0.05),但牧区藏族、城镇藏族、城镇汉族之间收缩压、血糖、血尿酸、TG、TC、LDL-C差异无统计学意义(P>0.05).3 500 m海拔中牧区藏族血糖及LDL-C高于2 400 m海拔中牧区藏族血糖及LDL-C(P<0.05或<0.01),而收缩压、舒张压、TG、HDL-C却低于2 400 m海拔中牧区藏族收缩压、舒张压、TG、HDL-C(P<0.05或<0.01).3 500 m海拔中城镇藏族舒张压、TG、HDL-C低于2 400 m海拔中城镇藏族舒张压、TG、HDL-C(P<0.05或<0.01).两个海拔中城镇汉族之间各指标差异无统计学意义(P>0.05).结论 海拔对牧区藏族及城镇藏族血压、血糖、血脂水平存在不同程度影响.     

高血黏度对小鼠脑细胞线粒体含量及超微结构的影响

目的：观察高血黏度对脑细胞线粒体含量及超微结构的影响。方法：采用高分子右旋糖酐法建立小鼠高血黏度动物模型,以Mito Tracker Green FM对线粒体进行标记,用激光扫描共聚焦显微镜测定脑细胞线粒体含量的变化;透射电镜观察小鼠大脑顶叶皮质及海马CA1区神经元线粒体超微结构的变化。结果：高血黏度小鼠脑细胞线粒体含量非常明显地减少;线粒体超微结构发生明显变化,且海马CA1区较皮质部变化明显。结论：高血黏度可导致脑细胞线粒体含量的减少及超微结构的变化。     

VP16诱导Raji肿瘤细胞凋亡的实验研究

目的 从细胞凋亡角度探讨ＶＰ１６抗肿瘤作用的机理。方法 用光学显微镜及电子显微镜观察了ＶＰ１６诱导Ｒａｊｉ细胞凋亡的形态学变化;用琼脂糖凝胶电泳观察了ＤＮＡ的梯形改变。结果 ＶＰ１６可明显诱导Ｒａｊｉ细胞调亡,５ｕｇ．ｍｌ＾－１ＶＰ１６作用４８ｈ时,凋亡指数为５８．３３％±３．０６％。与对照组相比有显性差异（Ｐ〈０．０１）。凋亡指数与药物浓度的对数呈正相关（ｒ＝０．９４０７,Ｐ〈０．０１）。结论 诱导凋亡是ＶＰ１６抗肿瘤作用的机理之一。