前胡丙素对高血压大鼠血管肥厚、细胞内钙、胶原及一氧化氮的影响

目的 研究前胡丙素对自发性高血压大鼠SHR及肾型高血压大鼠RHR的血管肥厚、细胞内钙、胶原、一氧化氮（NO）及血管反应性影响。方法 用显微测微仪测定血管中膜层，细胞大小，用Fura-2/AM为荧光指示剂，测定单细胞内[Ca^2 ]i，以测定羟脯氨酸含量反映胶原含量，用Griess法测定NO含量，以血管环观察收缩反应。结果 前胡丙素减轻血管中膜层增厚，维持细胞内[Ca^2 ]i稳态。减少胶原形成，增加SMCs释放NO。抑制血管环高反应状态。结论 前胡丙素抑制高血压血管肥厚，降低胶原含量及血管异常反应。     

重组多顺反子逆转录病毒介导的人载脂蛋白AI 与卵磷脂胆固醇脂酰基转移酶在肌源性细胞的表达

目的 :载脂蛋白ＡＩ(ａｐｏｌｉｐｏｐｒｏｔｅｉｎ ,ａｐｏＡＩ)与卵磷脂胆固醇脂酰基转移酶 (ｌｅｃｉｔｈｉｎ :ｃｈｏｌｅｓｔｅｒｏｌａｃｙｌｔｒａｎｓ ｆｅｒａｓｅ ,ＬＣＡＴ)作为高密度脂蛋白 (ｈｉｇｈｄｅｎｓｉｔｙｌｉｐｏｐｒｏｔｅｉｎ ,ＨＤＬ)的主要组分 ,在胆固醇由周围组织向肝脏的运输、排泄过程即胆固醇逆转运 (ｒｅｖｅｒｓｅｃｈｏｌｅｓｔｅｒｏｌｔｒａｎｓｐｏｒｔ,ＲＣＴ)过程中起关键作用。近年研究进一步证实 ,ＡｐｏＡＩ与ＬＣＡＴ的转基因动物能纠正由于遗传缺陷所致的低ＨＤＬ血症并有效抵抗高脂食物引起…     

冻干蜂王浆延缓衰老的实验研究

蜂王浆作为天然食品 ,被众多人们喜爱 ,临床验证长期服用具有提高机体免疫力 ,增强机体抗病能力 ,治疗便秘等疗效 [1 ]。本文以实验方法对其延缓衰老的作用机理进行探讨。1　材料与方法1.1　受试样品　冻干蜂王浆含片 ,淡黄色 ,0 .5 g/片 ,由某营养保健品有限公司提供 ,批号 :990 42 5。1.2　实验动物　 SD大鼠 ,2 0月龄 ,48只 ,由江苏省实验动物中心提供。1.3　试验方法　按卫生部颁布《保健食品功能学评价程序和检验方法》进行。(1)分组及给样 :试验大鼠 48只 ,随机均分 4组 ,每组 12只。通过灌胃每天分别给予冻干蜂王浆低 (15 0 mg/kg)…     

Targeted Expression and Secretion of Human Apoprotein AI, Apoprotein E and Lecithin cholesterol Acyltransferase from Myogenic Cells

Ａｔｈｅｒｏｓｃｌｅｒｏｓｉｓａｎｄｉｔｓｃｏｎｓｅｑｕｅｎｃｅｓａｃｃｏｕｎｔｆｏｒｍｏｓｔｍｏｒｂｉｄｉｔｙａｎｄｍｏｒｔａｌｉｔｙｉｎｔｈｅｗｏｒｌｄ.Ａｎｕｍｂｅｒｏｆｅｐｉｄｅｍｉｏｌｏｇｉｃａｌｓｔｕｄｉｅｓｈａｖｅｄｅｍｏｎ ｓｔｒａｔｅｄａｎｉｎｖｅｒｓｅｒｅｌａｔｉｏｎｓｈｉｐｂｅｔｗｅｅｎ ｐｌａｓｍａｈｉｇｈ ｄｅｎｓｉｔｙｌｉｐｏｐｒｏｔｅｉｎ (ＨＤＬ)ｃｏｎｃｅｎｔｒａｔｉｏｎａｎｄｔｈｅｉｎｃｉｄｅｎｃｅｏｆａｔｈｅｒｏｓｃｌｅｒｏｓｉｓ[1 ] .Ｉｔｉｓｗｉｄｅｌｙａｃ ｃｅ…     

银菊降脂茶调节大鼠血脂作用的实验研究

用实验性高脂血症大鼠研究了银菊降脂茶调节血脂的作用。结果显示：银菊降脂茶低、中、高剂量组大鼠血中胆固醇含量分别降低 １４３％ 、１８１０％ 、２６０ ０％ ，且呈剂量—效应关系，表明银菊降脂茶具有调节血脂的作用。     

Ⅰ型胶原与氧化性低密度脂蛋白相互作用的机制研究

目的：阐明胶原与血清低密度脂蛋白（ＬＤＬ）之间的关系。方法：在体外制备了Ⅰ型胶原凝胶,观察可能影响氧化ＬＤＬ（ｏｘ－ＬＤＬ）与胶原结合的几种因素。结果：随着ＬＤＬ氧化程度增加,其与胶原的结合量也增加。结论：这种增加可能主要源于脂蛋白的负电性增加,ＬＤＬ聚集度增大则可降低ＬＤＬ与胶原的结合能力。另外,载脂蛋白Ｂ中赖、精、组、酪氨酸残基的化学修饰,可能在ｏｘ－ＬＤＬ与胶原的结合中起重要作用     

Expression of Lecithin: Cholesterol Acyltransferase and/or apoA-I Mediated by Recombinant Adeno-associated Virus in Myogenic Cell

Ａｓａｋｅｙｐａｒｔｉｃｉｐａｎｔｉｎｔｈｅｐｒｏｃｅｓｓｏｆｒｅｖｅｒｓｅｃｈｏｌｅｓｔｅｒｏｌａｃｙｌｔｒａｎｓｆｅｒａｓｅ (ＲＣＴ)ａｎｄｐｌａｙｉｎｇａｎｉｍｐｏｒｔａｎｔｒｏｌｅｉｎｔｈｅｍａｔｕｒａｔｉｏｎｏｆＨＤＬａｎｄｍａｉｎｔａｉｎｉｎｇ     

Targeted Expression and Secretion of Human Apoprotein AI,Apoprotein E and Lecithin-choles-terol Acyltransferase from Myogenic Cell

Objective To investigate the possibility of heterologous expression for apoAI, apoE and LCAT by skeletal muscle cells and secretion into blood and to develop a safe and convenient gene therapy method for atherosclerosis. Methods Viral and nonviral vectors containing apoAI, apoE or LCAT genes were constructed and transfected into myogenic cells in vitro or injected directed into mouse skeletal muscle. The expression efficiencies of these vectors were investigated by ELISA assay for human apoAI and apoE3 and by the proteoliposome method for human LCAT. Genomic DNA was extracted from stable transduced myoblasts and analyzed for the presence of vector sequence by PCR amplifications. Immunocytochemistry assay was also performed to make an intuitionistic detection for the expression of transgene in myoblasts. Results All viral or nonviral vectors constructed in present study expressed the transgenes efficiently in mice skeletal muscles in vivo or cultured myoblasts in vitro. The transgene expression level of cells transfected with AAV-based plasmid vectors were 2-4 times higher then that of cells transfected with conventional plasmid vectors. Additionally, cells transfected with AAV-based bicistronic vector or tricistronic retroviral vector expressed both human apoAI and LCAT simultaneously. The sequences of retroviral or AAV-based plasmid vectors were found to be retained in host cells after transfection when that of conventional plasmid vectors were lost. Furthermore, transduced myoblasts maintained the ability for heterologous expression of human apoAI and LCAT even after differentiation into myotubes. For cells transfected with retroviral vectors, stable transduced clones can be selected by G418 and continued to efficiently express human apoAI and LCAT for 3 months. Conclusion These finds indicated that mice skeletal muscles or cultured myoblasts transduced with viral or non-viral vectors could efficiently express and secret human apoAI, apoE and LCAT. It suggested that the use of nonviral, adenoviral or AAV-based vectors to directly inject into skeletal muscle or the use of polycistronic retroviral to genetically modify myoblasts ex vivo and then implantation back to skeletal muscle to high efficiently and long-term express apoAI, apoE and LCAT in vivo might be a safe and feasible strategy to prevent or reduce the formation of atherosclerotic lesions.     

银杏内酯对血管紧张Ⅱ依赖性血管平滑肌细胞增殖的作用

目的 观察血管紧张Ⅱ（AngⅡ）对培养牛主动脉血管平滑肌细胞（VSMC）增殖的作用及银杏内酯对其增殖的影响。方法 采用组织块贴壁法培养牛主动脉VSMC,以^3H-TdR掺入法和流式细胞仪分别测定VSMC的DNA合成量及细胞周期中各期细胞构成比。结果 （AngⅡ）（0.1μmol/L)作用24h使用^3H-TdR掺入量比对照组增加34%;S期和G2/M期细胞增多,细胞增殖指数由35.15%增至45.03%。与（AngⅡ）组相比,AngⅡ加银杏内酯B（G-B,0.1-10μmol/L)组、AngⅡ加G-B和银杏内酯A（G-A）的混合物（G-AB,0.1-10μmol/L)组的^3H-TdR掺入量减少;S期细胞构成比和细胞增殖指数减少。结论 AngⅡ对VSMC有促增殖作用,要被G-B和G-AB所拮抗。     

4-吗啉乙腈盐酸盐的抗人低密度脂蛋白氧化作用

目的　观察 4 吗啉乙腈盐酸盐及其衍生物的抗氧化特性。方法　分别在铜离子和 2 ,2′ 盐酸脒基丙烷 (AAPH)催化的低密度脂蛋白 (LDL)氧化反应中加入 4 吗啉乙腈盐酸盐 ,以共轭二烯、丙二醛的生成和相对电泳迁移率作为测定指标 ,并比较 4 吗啉乙腈盐酸盐等系列化合物的抗氧化效果。结果　 4 吗啉乙腈盐酸盐可使LDL氧化反应的潜伏期明显延长 ,LDL中共轭二烯和脂质过氧化产物的生成明显减少 ,相对电泳迁移率下降 ,上述作用均随 4 吗啉乙腈盐酸盐浓度增加而进一步加强。结论　 4 吗啉乙腈盐酸盐具有抗人低密度脂蛋白氧化作用