细胞骨架中间丝包括神经细丝蛋白、角蛋白、波形蛋白单克隆抗体的制备和应用

本文主要制备抗3种类型中间丝的单克隆抗体;从人胼胝和HeLa细胞中分别提取角蛋白,从牛脊髓中提取神经细丝蛋白,从人胎脑中提取波形纤维蛋白,纯化后免疫小鼠,经细胞融合,筛选和鉴定,并进行亚克隆培养,分别证明获得2株特异性不同的抗     

抗神经细丝蛋白单克隆抗体杂交瘤细胞株的建立与鉴定

本实验应用 Triton X-100处理,羟基磷灰石初步纯化和 DEAE 纤维素层析的方法,从牛脊髓中提取神经细丝蛋白,免疫小鼠,经细胞融合与鉴定,证明所能分泌抗神经细丝蛋白单克隆抗体杂交瘤细胞株特异性高,性质稳定,可以用于细胞学、组织学、病理学等方面的研究。     

GENERATION AND CHARACTERIZATION OF TWO MONOCLONAL ANTIBODIES AGAINST CYTOKERATINS

Thirty-five hybridoma cell lines against cytoke-ratins, isolated from Hela cells and human cellus respectively, were generated by fusion of immunized spleen cells of BALB/C mice with P3×63-Ag8.653, a mouse myeloma cell line. Two of them (HI and C53) were characterized by indirect immu-nofluorescence, ABC immunostaining and immuno-blotting. The results of immunofluorescence and ABC immunostaining suggested that both monoclonal antibodies were specific for keratin-type intermediate filaments. However, the two monoclonal antibodies showed different specificities in normal tissues and neoplasms as observed on both frozen and deparaf-finized sections. In normal tissues, H1 stained transitional epithelium and all types of simple epithelium except endothelium and mesothelium but did not stain stratified squamous epithelium. In contrast, C35 recognized only stratified squamous epithelium, but failing to react with simple epithelium. Both monoclonal antibodies did not react with nonepithelia cells and tissues.     

复发性急性胰腺炎的临床特征及影像学表现分析

目的:探讨复发性急性胰腺炎(recurrent acute pancreatitis, RAP)的临床特征及其影像学表现。方法:回顾性分析2019年11月至2021年11月我院收治RAP患者的临床及影像资料，并复习国内外相关文献。结果:(1)RAP患者以中年男性为主，致病因素依次是高脂血症、特发性、胆道疾病、酒精性以及其他罕见疾病，约半数RAP患者伴有基础疾病，并且多数患者既往有2次及以上急性胰腺炎病史；(2)RAP以间质水肿型胰腺炎为主，局部并发症包括胰周积液18例、假性囊肿5例、胰周坏死物聚集14例、包裹性坏死9例；(3)老年RAP中女性明显多于男性，男女比1:3,主要病因是胆源性疾病。结论:RAP好发于中年男性，高脂血症是其首位病因，多数患者多次复发并以轻症胰腺炎为主。