补阳还五汤减轻氧化应激保护脑缺血再灌注损伤大鼠血脑屏障的作用研究

目的 探究补阳还五汤(Buyang Huanwu Decoction, BYHWD)降低氧化应激水平保护脑缺血再灌注损伤(cerebral ischemia/reperfusion injury, CIRI)大鼠血脑屏障(blood-brain barrier, BBB)的机制。方法 采用线栓法建立大鼠大脑中动脉栓塞再灌注(middle cerebral artery occlusion/reperfusion, MCAO/R)模型,应用PeriCam PSI激光散斑血流成像系统检测脑血流量判定模型建立是否成功。通过Zea Longa评分评价大鼠神经功能缺损情况;HE染色观察大鼠脑组织病理学改变;干湿重法检测脑水肿程度;伊文思蓝(Evans blue, EB)染色检测BBB通透性;透射电镜观察BBB超微结构改变;试剂盒检测氧化应激相关指标活性氧(ROS)、丙二醛(MDA)的含量及总超氧化物歧化酶(SOD)活性;免疫组织化学染色及Western blot检测基质金属蛋白酶-9(MMP-9)的表达水平;免疫荧光双染及Western blot检测紧密连接蛋白(tight junction p...     

补阳还五汤通过SIRT1/NF-κB p65信号通路减轻大鼠脑缺血/再灌注损伤

目的：探讨补阳还五汤（BYHWD）通过调控沉默信息调节因子1(SIRT1)/核因子κB p65(NF-κB p65)炎症信号通路减轻大鼠脑缺血/再灌注损伤（CIRI）的作用机制。方法：SD大鼠随机分为假手术（sham）组、模型组（MCAO/R组）、BYHWD组、SIRT1抑制剂组（EX527组）和BYHWD+EX527组,每组15只。除sham组外,其余各组均构建大脑中动脉栓塞（MCAO）模型缺血2 h再灌注3 d并给予药物干预。采用Zea Longa评分标准进行神经功能缺损评分;TTC染色检测脑梗死体积百分比;HE染色观察脑组织病理学变化;Western blot检测SIRT1、乙酰化核因子κB p65(Ac NF-κB p65)、NF-κB p65、和白细胞介素6(IL-6)蛋白的相对表达量;免疫荧光检测NF-κB p65入核表达水平;免疫组化染色检测肿瘤坏死因子α(TNF-α)表达水平。结果：与sham组相比,MCAO/R组神经功能学评分升高（P<0.05）;脑梗死体积百分比增加（P<0.05）;缺血侧脑组织皮质区病理损伤严重,神经细胞排列紊乱、细胞核固缩、碎裂;SI...     

Mechanism of Buyang Huanwu Decoction alleviating inflammatory response after cerebral ischemia/reperfusion injury by activating SIRT1 in hippocampus of rats北大核心CSCD

Aim To explore the inhibitory effect of Buyang Huanwu Decoction on the inflammatory response in the hippocampus of brain tissues of CIRI rats by regulating SIRT1 and the underlying mechanism. Methods The middle cerebral artery embolization (MCAO) model was prepared in rats and divided into sham operation group (Sham), model group (MCAO/R), Buyang Huanwu Decoction group (BYHWT),and BYHWT + SIRT1 inhibitor group (BYHWT + EX527). Zea Longa was used to detect the neurological function score of rats in each group; TTC staining was used to determine the volume of cerebral infarction; HE staining was used to observe the pathological damage of the hippocampus; Western blot was used to detect the expression levels of SIRT1 and IL-6; immunohistochemistry was used to detect TNF-α, IL-1β expression level. Results Compared with the sham group,the neurological function score of the MCAO/R group increased (P < 0.05); the volume of cerebral infarction increased (P < 0.05); the nerve cells in hippocampus were severely damaged, arranged disorderly, and the nucleus was broken; Western blot showed that the expression of SIRT1 decreased, IL-6 expression increased (P <0.05); immunohistochemistry showed that TNF-α,IL-1β expression increased (P < 0.05). Compared with the MCAO/R group, the neurological function score of the BYHWT group decreased (P <0.05); the volume of cerebral infarction decreased (P < 0.05); the damage of nerve cells in hippocampus was reduced; Western blot showed that the expression of SIRT1 increased and IL-6 expression decreased (P < 0.05); immunohistochemistry showed that TNF-α, IL-1β expression decreased (P < 0.05). Compared with the BYHWT group, the neurological function score of the BYHWT + EX527 group increased (P < 0.05); the volume of cerebral infarction was raised (P <0.05); the damage of nerve cells in hippocampus was aggravated; Western blot showed that the expression of SIRT1 decreased and IL-6 expression increased (P < 0.05); immunohistochemistry showed that TNF-α, IL-1β expression increased (P < 0.05). Conclusions Preliminary discussion of Buyang Huanwu Decoction can activate SIRT1 in hippocampus of rat brain tissues to reduce the inflammatory response after CIRI and play a role in brain protection. © 2023 Publication Centre of Anhui Medical University. All rights reserved.