细胞骨架中间丝包括神经细丝蛋白、角蛋白、波形蛋白单克隆抗体的制备和应用

本文主要制备抗3种类型中间丝的单克隆抗体;从人胼胝和HeLa细胞中分别提取角蛋白,从牛脊髓中提取神经细丝蛋白,从人胎脑中提取波形纤维蛋白,纯化后免疫小鼠,经细胞融合,筛选和鉴定,并进行亚克隆培养,分别证明获得2株特异性不同的抗     

胃肠肿瘤中H-ras癌基因第十二位密码子点突变的研究

采用聚合酶链反应和寡核苷酸探针杂交法检测20例胃肠肿瘤中H-ras癌基因第十二位密码子的G→T点突变,结果在1/1贲门癌和4/13胃癌中发现有这种点突变的存在,而6例大肠癌中无1例发生突变,提示H—ras癌基因的点突变可能是贲门癌和胃癌中癌基因的激活方式之一。     

GENERATION AND CHARACTERIZATION OF TWO MONOCLONAL ANTIBODIES AGAINST CYTOKERATINS

Thirty-five hybridoma cell lines against cytoke-ratins, isolated from Hela cells and human cellus respectively, were generated by fusion of immunized spleen cells of BALB/C mice with P3×63-Ag8.653, a mouse myeloma cell line. Two of them (HI and C53) were characterized by indirect immu-nofluorescence, ABC immunostaining and immuno-blotting. The results of immunofluorescence and ABC immunostaining suggested that both monoclonal antibodies were specific for keratin-type intermediate filaments. However, the two monoclonal antibodies showed different specificities in normal tissues and neoplasms as observed on both frozen and deparaf-finized sections. In normal tissues, H1 stained transitional epithelium and all types of simple epithelium except endothelium and mesothelium but did not stain stratified squamous epithelium. In contrast, C35 recognized only stratified squamous epithelium, but failing to react with simple epithelium. Both monoclonal antibodies did not react with nonepithelia cells and tissues.