抗人肺腺癌单克隆抗体在荷瘤裸鼠体内的分布及肿瘤放射免疫显象

Biodistribution and radioimmunoimaging of monoclonal antibody, 5F11, against human lung adenocarcinoma (LTEP-a2) in tumor-bearing nude mice were studied. The binding rate of radioiodinated 5F11 with LTEP-a2, as determined by in vitro binding assay, was 27.64 +/- 5.01%, while the control was less than 5.0%, suggesting that 5F11 reacted specifically with LTEP-a2. Seventy-two, 96 and 120 hr after injection of 125I-5F11, the percentage of dose taken per gram tumor tissue was 4.84%, 6.29% and 6.60%, higher than those of all normal organs. From 48 to 120 hr on, the ratio of tumor to normal tissue (T/NT) in the liver, spleen, kidney, stomach and intestine was more than 2; T/NT in the lung was 3.1-4.7; T/NT in the blood increased gradually and was 1.87 at 96 after injection. Tumor location index was 4.16, while that of the normal tissue was only 1.21. These results showed that human lung adenocarcinoma xenografts in nude mice were specifically located by radioiodinated 5F11. Radioimmunoimaging was performed in tumor-bearing nude mice. The tumors were clearly visualized 72 hr after injection of 131I-5F11. Radioactivity was higher in tumor region than in other regions. The optimal imaging time was 72-96 hr after injection of radioiodinated 5F11.     

抗人肺癌单克隆抗体的研究

用人肺腺癌LTEP-a_2细胞免疫BALB/c小鼠取其脾细胞与Sp/0骨髓瘤细胞融合,用间接免疫荧光染色法在a_2癌细胞及人胚肺二倍体纤维母细胞2BS上进行筛选,得到2A_7、2E_9、4F_2、5F_(11)四个杂交瘤,这些抗体主要识别人肺癌细胞,与2BS及包括四种不同ABO血型的10个人红血球和淋巴细胞无反应,用ABC免疫酶染色试验检查了四种单克隆抗体与     

人肺癌标本中c-myc癌基因扩增的研究

 分子生物学在医学领域中的应用日渐扩展,从基因水平来研究肿瘤的发生、发展成为可能,并已取得一定的进展,现已明了,myc基因的扩增是人肺癌细胞的重要的分子改变之一。     

热疗联合放疗或化疗治疗晚期非小细胞肺癌63例临床分析

探讨热疗联合放疗和化疗治疗63例晚期非小细胞肺癌(non-small cell lung cancer，NSCLC)患者的临床疗效。     

抗人肺腺癌单克隆抗体放射免疫显像的动物实验研究

本文介绍用单克隆抗体进行裸鼠移植瘤的放射免疫定位的实验结果。用人肺腺癌细胞株(LTEP-a_2)免疫BALB/c小鼠,取脾细胞与Sp2/0细胞融合,筛选出产生抗LTEP-a_2抗体的杂交瘤细胞,经3次亚克隆后,注入BALB/c小鼠腹腔,产生腹水。Zetaprep离子交换     

内生场热疗治疗55例晚期肺癌的临床分析

随着电子技术的迅速发展及临床医学工作者的不懈努力，热疗取得了较快的发展，该方法是继手术、放疗、化疗、免疫疗法后第五种肿瘤治疗方法。目前对浅表肿瘤的热疗研究进行得较为广泛，并取得确切的疗效，但对深部肿瘤的加温治疗尤其是肺癌的热疗临床报道尚不多。本文报告了应用国产NRL-001型内生