HIF-1和BFGF、BFGFR与大肠癌的关系

缺氧诱导因子-1（Hypoxia inducible factor-1，HIF-1）是缺氧条件下广泛存在于哺乳动物和人体内的一种转录因子。大量的研究表明HIF-1在大肠癌组织中高表达，且与肿瘤的多种生物学行为有相关性，说明HIF-1在大肠癌的发病机制中扮演着一个相当重要的角色。细胞生长因子与肿瘤的关系已日益受到重视。多种生长因子在不同的肿瘤组织或体液中可被检测；多种细胞生长因子的受体在某些肿瘤组织中高表达；     

Expression of hypoxia inducible factor-1α and basic fibroblast factor in the tissues of colorectal adenocarcinoma

Objective To observe expression of hypoxia inducible factor-1α(HIF-1α)and basic fibroblast growth factor(BFGF)in the tissues of colorectal adenoearcinoma and analyze the relationship between expression of the two factors and biological behavior of colorectal adenocarcinoma.Methods The samples of colorectal adenocarcinoma(n=60)and para-adenocarcinoma(n=60)were taken from surgical resection patients and normal colorectal tissues (n=20) from patients with irritable bowel syndrome.The expression of HIF-1α and BFGF were detected by immunohistochemical staining (SP method).Results Positive rates of HIF-1αand BFGF in colorectal adenocarcinoma tissues were 61.7% and 65.0%,and positive rates of HIF-1α and BFGF in para-adenocarcinoma tissues were 10.0%and 13.3%(P<0.05,respectively);HIF-1αand BFGF were not detected in normal intestinal mucosa.There was no significant correlation with HIF-1α and BFGF expression in colorectal adenocarcinoma tissues to the sexuality,age,tumor size,tumorposition and differentiation(P>0.05).A significant correlation between expression of the two factors and Dukes stage was observed (P<0.05).Positive rates of HIF-1α and BFGF in colorectal adenocarcinoma tissuesof Dukes A and B stage were 47.2%and 52.7%,respectively.Positive rates of HIF-1α and BFGF in colorectal adenocarcinoma tissues of Dukes C and D stage were 83.3%and 83.3%.respectively.There was a positive correlation between expression of the two factors and carcinogenesis of colorectal adenocarcinoma(r=0.4276.P<0.001).Conclusion The results showed that the enhanced expression of HIF-1 α and BFGF incolorectal adenocarcinoma tissues may be associated with the prognosis of the patients with colorectal adenocarcinoma,and HIF-1α and BFGF may participate synergistically in the carcinogenesis of colorectal adenocarcinoma.     

学术导向性信息在科研工作中的应用

学术导向性信息是亟待开发利用的一种新型服务信息。笔者对学术导向性信息的概 念、收集筛选、开发应用等方面进行探讨,对促进临床科研工作具有一定的指导意义。     

结直肠腺癌组织中HIF-1α与BFGFR的表达

目的 研究缺氧诱导因子1α(HIF-1α)和碱性成纤维牛长因子受体(BFGFR)在结直肠腺癌中的表达和相瓦作用,以及它们与结直肠腺癌牛物学行为的关系.方法 采用免疫组化SP法检测了60例手术切除的结直肠腺癌及癌旁组织、20例正常大肠黏膜组织中的HIF-1α和BFGFR表达.结果 HIF-1 α和BFGFR在结直肠腺癌中的表达阳性率分别为61.7%和58.3%,明显高于癌旁组织的10.0%和11.7%(P＜0.05).正常肠黏膜中未检出HIF-1α、BFGFR,HIF-1α和BFGFR在结直肠腺癌中表达与性别、年龄、肿瘤大小、肿瘤位置和分化程度无关(P＞0.05),但与肿瘤Dukes分期相关(P＜0.05).结直肠腺癌组织中HIF-1α与BFGFR表达具有相关性(P＜0.05).结论 结直肠腺癌组织中的HIF-1α和BFGFR表达水平增高,参与了结直肠腺癌的发生发展过程;且HIF-1α与BFGFR可能起到协同作用,共间促进肿瘤的发生.     

内镜下高频电凝联合根除幽门螺杆菌治疗隆起糜烂性胃炎的临床研究

目的 探讨应用内镜下高频电凝联合根除幽门螺杆菌(Hp)治疗隆起糜烂性胃炎(EGP)的价值和安全性.方法 将60例EGP患者分成A组(抑酸、保护胃黏膜、根除Hp药物治疗)和B组(抑酸、保护胃黏膜、根除Hp药物+高频电凝治疗同时进行)各30例,比较两种方法 的疗效.结果 A组单纯药物治疗后临床症状缓解(P＜0.05),但隆起病灶数消失不明显,仅有18.57%(47/253)的隆起病灶消失;B组治疗后,临床症状明显缓解(P＜0.05),97.15%(273/281),隆起病灶消失(P＜0.05),与单纯药物治疗组比较差异有统计学意义(P＜0.05).有6例出现轻微上腹胀或上腹隐痛,均在术后5 d内消失.结论 EGP单纯药物治疗疗效欠佳,内镜下高频电凝联合根除Hp治疗EGP不仅疗效确切,而且安全简便.     

Expression of hypoxia inducible factor-1α and basic fibroblast factor in the tissues of colorectal adenocarcinoma

Objective To observe expression of hypoxia inducible factor-1α(HIF-1α)and basic fibroblast growth factor(BFGF)in the tissues of colorectal adenoearcinoma and analyze the relationship between expression of the two factors and biological behavior of colorectal adenocarcinoma.Methods The samples of colorectal adenocarcinoma(n=60)and para-adenocarcinoma(n=60)were taken from surgical resection patients and normal colorectal tissues (n=20) from patients with irritable bowel syndrome.The expression of HIF-1α and BFGF were detected by immunohistochemical staining (SP method).Results Positive rates of HIF-1αand BFGF in colorectal adenocarcinoma tissues were 61.7% and 65.0%,and positive rates of HIF-1α and BFGF in para-adenocarcinoma tissues were 10.0%and 13.3%(P<0.05,respectively);HIF-1αand BFGF were not detected in normal intestinal mucosa.There was no significant correlation with HIF-1α and BFGF expression in colorectal adenocarcinoma tissues to the sexuality,age,tumor size,tumorposition and differentiation(P>0.05).A significant correlation between expression of the two factors and Dukes stage was observed (P<0.05).Positive rates of HIF-1α and BFGF in colorectal adenocarcinoma tissuesof Dukes A and B stage were 47.2%and 52.7%,respectively.Positive rates of HIF-1α and BFGF in colorectal adenocarcinoma tissues of Dukes C and D stage were 83.3%and 83.3%.respectively.There was a positive correlation between expression of the two factors and carcinogenesis of colorectal adenocarcinoma(r=0.4276.P<0.001).Conclusion The results showed that the enhanced expression of HIF-1 α and BFGF incolorectal adenocarcinoma tissues may be associated with the prognosis of the patients with colorectal adenocarcinoma,and HIF-1α and BFGF may participate synergistically in the carcinogenesis of colorectal adenocarcinoma.     

Expression of hypoxia inducible factor-1α and basic fibroblast factor in the tissues of colorectal adenocarcinoma

Objective To observe expression of hypoxia inducible factor-1α(HIF-1α)and basic fibroblast growth factor(BFGF)in the tissues of colorectal adenoearcinoma and analyze the relationship between expression of the two factors and biological behavior of colorectal adenocarcinoma.Methods The samples of colorectal adenocarcinoma(n=60)and para-adenocarcinoma(n=60)were taken from surgical resection patients and normal colorectal tissues (n=20) from patients with irritable bowel syndrome.The expression of HIF-1α and BFGF were detected by immunohistochemical staining (SP method).Results Positive rates of HIF-1αand BFGF in colorectal adenocarcinoma tissues were 61.7% and 65.0%,and positive rates of HIF-1α and BFGF in para-adenocarcinoma tissues were 10.0%and 13.3%(P<0.05,respectively);HIF-1αand BFGF were not detected in normal intestinal mucosa.There was no significant correlation with HIF-1α and BFGF expression in colorectal adenocarcinoma tissues to the sexuality,age,tumor size,tumorposition and differentiation(P>0.05).A significant correlation between expression of the two factors and Dukes stage was observed (P<0.05).Positive rates of HIF-1α and BFGF in colorectal adenocarcinoma tissuesof Dukes A and B stage were 47.2%and 52.7%,respectively.Positive rates of HIF-1α and BFGF in colorectal adenocarcinoma tissues of Dukes C and D stage were 83.3%and 83.3%.respectively.There was a positive correlation between expression of the two factors and carcinogenesis of colorectal adenocarcinoma(r=0.4276.P<0.001).Conclusion The results showed that the enhanced expression of HIF-1 α and BFGF incolorectal adenocarcinoma tissues may be associated with the prognosis of the patients with colorectal adenocarcinoma,and HIF-1α and BFGF may participate synergistically in the carcinogenesis of colorectal adenocarcinoma.     

复方阿米卡星凝胶预防留置尿管逆行感染的研究

为探讨预防留置尿管逆行感染(UTIC)的有效措施,将100例留置尿管>10 d的病人,随机分为观察组和对照组各50例,按双盲法操作.观察组应用自行研制的复方阿米卡星凝胶替代石蜡油作尿管润滑剂,置管期间 ,行尿道口清洁后用凝胶均匀涂于尿道口周围,2次/d;对照组按常规留置尿管及护理.比较两组病人置管期间尿细菌培养阳性病例数.结果留置尿管40 d,观察组发生UTIC 2例,对照组发生30例,两组比较,χ2=36.02,P<0.01,差异有极显著性意义.提示复方阿米卡星凝胶能有效防止UTIC的发生;该凝胶取材容易,价格低廉,护理操作简便.     

超声检查在鼻咽癌颈淋巴结转移诊断中的应用

目的探讨经颈超声检查在鼻咽癌及其颈淋巴结转移诊断中的应用价值。方法以CT作为参照,对4例病理已确诊为鼻咽癌的住院患者进行鼻咽部和左右侧颈部超声检查。患者取仰卧位,检查时头略向对侧倾斜,以腮腺及咽旁间隙作为透声窗,取下颌角和乳突间的耳垂下斜横断面和下颌骨升支旁的斜冠状切面显示鼻咽部,测量病理情况下鼻咽部软组织的厚度或肿块的范围,观察其形态、边界、内部回声及与周围组织的关系,然后再用高频探头检查左右侧颈部,并对肿大的淋巴结测量记录。结果4例患者显示6个病灶,其中单侧病灶2例,双侧病灶2例。病灶中低回声4个,中等回声2个,实质性回声6个,夹杂液无回声区1个;声像图表现鼻咽部软组织增厚4个,鼻咽部肿块2个;超声提示4例颈部淋巴结均肿大。结论经颈超声扫查可观察到鼻咽癌相应的鼻咽部软组织异常增厚或肿块的声像表现,有助于鼻咽癌颈部淋巴结转移的诊断。     

超声引导下经皮肺穿刺活检的临床意义

目的探讨超声引导下经皮肺穿刺活检的临床意义。方法对超声能观察显示不明性质的83例肺部病变行超声引导下用穿刺活检。超声检查确定病灶部位后,在超声引导下穿刺活检针进入肺部病灶的边缘取活组织,将组织送病理组织学检查。结果 83例肺部病交取材病理阳性78例,阳性率为93．98％,其中周围型肺癌51例,肺结核瘤14 例,肺炎性假瘤9例,肺炎性实变4例,胸膜闻皮瘤3例,狼疮肺1例,结核性胸膜炎1例,术后并发症为2．4％(2／83)。结论超声引导经皮肺穿刺活检为临床提供了有助于明确病因的诊断依据,对临床诊断、手术治疗和判断预后均有重要价值和指导作用,而且费用低,值得基层医院推广。