减体积肝移植模型大鼠肝脏组织miRNAs表达谱变化

BACKGROUND: Liver regeneration is the key factor influencing the prognosis of living donor liver transplantation. There has not been the research on special miRNA of liver regeneration after living donor liver transplantation. OBJECTIVE: To analyze the variation of miRNAs expression profile after rat reduced-size liver transplantation at certain time point, select and verify target miRNA which can provide targeting intervention strategies in liver regeneration after rat reduced-size liver transplantation and provide theoretical evidence for liver regeneration after living donor liver transplantation. METHODS: The reduced-size liver transplantation models were established. miRNAs microarray was used to detect miRNA expression. In differentially expressed microRNAs, real-time quantitative PCR was utilized to detect target miRNAs. The credibility of miRNAs microarray results was verified. RESULTS AND CONCLUSION: Compared with rat liver tissue in the sham operation group, 11 miRNAs up-regulated in reduced-size liver transplantation, including let-7b-5p, let-7c-5p, miR-101a-3p, miR-103-3p, miR-130a-3p, miR-142-5p, miR-186-5p, miR-199a-3p, miR-21-5p, 221-3p and miR-34a-5p. Four miRNAs were down-regulated, including miR-26b-5p, miR-150-5p, miR-19a-3p and rno-miR-146-5p. PCR test further verified that miR-221-3p and miR-199a-3p expression changes approximated the chip results at 24, 48 hours and 1 week, indicating that results of miRNA microarray were believable. These results verified that it exists variation of miRNAs expression profile after rat reduced-size liver transplantation, which picked out and verified the target miRNAs.        

系统性红斑狼疮合并直肠穿孔1例报道

病例资料 患者,女,48岁.因全腹痛12h入院.疼痛呈持续性钝痛,伴腹胀、发热,恶心、呕吐2次,呕吐物为胃内容物.患者有“系统性红斑狼疮(SLE)、类风湿病”史约5年,长期服用强的松(10mg/d).查体:T37.8℃,生命体征平稳;急性病容,全身可见散在紫癜,未见出血点;浅表淋巴结无肿大;腹部平坦,未见胃肠形及蠕动波,全腹压痛明显,并反跳痛及肌紧张,移动性浊音阳性,肝区叩痛阳性,肠鸣音弱.Hb 136 g/L.WBC 8.7×109/L,N 0.81.患者入院后行急诊腹部立位X线平片检查,提示双膈下少量游离气体、不完全性肠梗阻.     

IL-17在大鼠肝移植急性排斥反应模型中的表达及作用研究

目的:探讨IL-17在大鼠肝移植急性排斥反应模型中的表达及作用。方法:选取32对健康状况相同的大鼠,随机分组后建立大鼠肝移植急性排斥反应动物模型。肝移植手术后取受体鼠的血浆标本和肝组织标本,对比两组受体大鼠的急性排斥反应,并测定两组大鼠的IL-17水平。结果:相较于异基因移植组受体大鼠而言,同基因移植组受体大鼠ALT和TBIL的平均含量更接近正常水平,急性排斥反应较弱。同时,同基因移植组受体大鼠术后IL-17水平明显高于异基因移植组,组间比较具有明显差异,P<0.05。结论:IL-17参与到大鼠肝移植急性排斥反应的发生过程,临床上可将IL-17水平作为急性排斥反应的衡量指标,以便及时发现移植受体的急性排斥反应,并及早作出有效应对。