携带腓肠神经营养血管的改良型腓动脉穿支螺旋桨皮瓣修复足跟部创面

目的探讨携带腓肠神经营养血管的改良型腓动脉穿支螺旋桨皮瓣修复足跟缺损的效果。方法对15例足跟部较大范围缺损(8 cm×12 cm～14 cm×20 cm)患者采用携带腓肠神经营养血管的改良型腓动脉穿支螺旋桨皮瓣进行修复,腓动脉穿支血管蒂直接旋转改为呈C形盘绕式。观察转移皮瓣成活等情况;末次随访时采用Maryland足部评分对术后疗效进行评价。结果术后1例皮瓣的远端发生宽约1 cm坏死; 1例皮瓣的近端边缘宽约1 cm坏死;其余皮瓣顺利成活,创面一期愈合。13例获得随访,时间7～72个月。患者行走无疼痛或仅轻微疼痛。末次随访时,皮瓣两点辨别觉15～25 mm,深感觉均恢复,皮瓣足底负重区无压疮形成,患者较为满意; Maryland足部评分75～95分,优9例,良3例,可1例。结论携带腓肠神经营养血管的改良型腓动脉穿支螺旋桨皮瓣手术操作简单,皮瓣蒂无肿胀,切取面积大,足跟负重区溃疡发生率低,适用于修复足跟部较大范围皮肤软组织缺损。     

我国8种雌蝇尾器光镜与扫描电镜形态特征研究

我国８种雌蝇尾器光镜与扫描电镜形态特征研究长春白求恩医科大学寄生虫学教研室（１３００２１）荆凯，易世红，张永生白求恩医科大学附属二院兰继毓长春市卫生专科学校李雅琴长春市朝阳区卫生防疫站张春燕，马英花众所周知，雄蝇尾器（ｍａｌｅｔｅｒｎｕｎａｌｉａ）在...     

腺病毒及腺相关病毒介导的自杀基因疗法对肿瘤的杀伤作用

用病毒治疗肿瘤是一种日益引起人们重视的肿瘤治疗手段 [1 ] 。早在 2 0世纪5 0年代 ,人们就开始利用腺病毒 (aden-ovirus,Ad)的细胞毒性进行治疗宫颈癌的试验。自杀基因疗法的原理是将“自杀”基因转移入宿主细胞的基因组中 ,这种基因编码的酶能使非活性的前药转化为细胞毒性的代谢物 ,诱导靶细胞产生“自杀”效应 ,从而达到清除肿瘤细胞的目的。Ad及 AAV介导的基因转移系统因其独特的生物学特性得到广泛的应用。1　腺病毒及腺相关病毒腺病毒基因组为线性双链 DNA ,长36 kb,10 0 m u(m apunit)。Ad基因组由非结构性早期基因 (E1 ～ E4…     

发热、腹痛、腹部包块

患者，女，87岁。因发热10天，腹痛伴恶心、呕吐4天于2002年4月29日入院。入院10天前无明显诱因出现发热，体温在39℃左右，在村卫生室按感冒给予青霉素、病毒唑等药物治疗，体温仍波动在38℃-39℃之间。4天前病人出现全腹疼痛，以中下腹部为重，呈阵发性腹痛，持续性加重，伴恶心，呕吐胃内容物，未做其他检查，改青霉素为先锋霉素消炎，并给予止痛对症处理，症状无明显缓解，遂来我院就诊。     

miR-195对人骨髓间充质干细胞成骨分化的影响

BACKGROUND:Previous studies have found that the expression level of miR-195 in differentiated human bone marrow mesenchymal stem cells (hBMMSCs) is significantly higher than that in undifferentiated hBMMSCs. However, miR-195 effect during this differentiation process and possible mechanism remain unclear. OBJECTIVE:To explore the effect of miR-195 on osteogenic differentiation of hBMMSCs and possible mechanism. METHODS:hBMMSCs were isolated and cultured in vitro. Alkaline phosphatase activity, Runx2, osteopontin and SMAD7 protein expression and miR-195 expression level during osteogenic differentiation of hBMMSCs were determined by alkaline phosphatase kit, western blot and real-time PCR, respectively. miR-195-downexpressed hBMMSCs were constructed by lipofection transfection, and were used to investigate the effect of miR-195 was on osteogenic differentiation of hBMMSCs. Dual luciferase reporter assay was used to identify whether the 3’UTR of SMAD7 mRNA was a binding target of miR-195. In addition, we transfected hBMMSCs with SMAD7 cDNA (pcDNA-SMAD7), and investigated the effect of SMAD7 on osteogenic differentiation of hBMMSCs. RESULTS AND CONCLUSION:The isolated and cultured hBMMSCs had good osteogenic differentiation ability in vitro. Expression level of miR-195 was increased with the increasing of induction time, and the expression level of SMAD7 was reversed. miR-195 promoted osteogenic differentiation of hBMMSCs. Luciferase assay confirmed that miR-195 targeted SMAD7 directly, and overexpression of SMAD7 inhibited the osteogenic differentiation of hBMMSCs. Taken together, miR-195 promotes osteogenic differentiation of hBMMSCs by targeting SMAD7.