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Objective To study the protective effects of adenovirus-mediated human beta-nerve growth factor gene (hNGFβ) transfer combined with iron fortified nutrition on blast hearing damage in guinea pigs. Methods Deafness was induced by blast (172dB SPL) in 35 healthy guinea pigs. Seven days after noise exposure, 10 guinea pigs were inoculated with adenovirus-mediated hNGFβ (Ad-hNGFβ) into the perilymphatic space (the gene group), another 10 guinea pigs were given hNGFβ combined with iron fortified nutritional diet (the combination group), still another 10 guinea pigs were inoculated with artificial perilymphatic fluid (APF), and 5 guinea pigs served as control, given neither medicine nor noise exposure. Auditory brainstem response (ABR) threshold shifts were monitored in the guinea pigs before blast exposure and after gene transfer. Changes in cochlear morphology were examined with immunohistochemical and HE staining. Results One week after successful inoculation of hNGFβ, it was noted that Ad-hNGFβ protein was expressed in each turn of the cochlea, and its intensity was almost equal. After gene transfer in the combination group and the gene group, ABR threshold shifts at week 1 and 4 were significantly smaller than those of the APF group. HE staining showed that the number of the spiral ganglion cells in the combination group and the Ad-hNGFβ group was significantly higher than that of the control group after 4 weeks (P<0.01). Conclusions Ad-hNGFβ combined with iron fortified nutritional diet was effective in the prevention and treatment of blast hearing damage of cochlear hair cells. 相似文献
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范静平 《国外医学:耳鼻咽喉科学分册》1993,(6)
内淋巴囊在梅尼埃病的病理生理学上可能起到很重要的作用,该文介绍一种获取和处理新鲜的人内淋巴管标本和有可能在手术中获取更具活性的内淋巴囊骨内部分的方法。具体方法是:在经迷路进路到达内听道的手术中,先在后颅窝靠近前庭导水管的外孔处将其辨认出来。用小金钢钻将前庭导水管轮廓化,直至其前庭处,并沿它的周围留下一层薄的骨壁。为了避免手术损伤脑膜和增加通入后颅窝的入口, 相似文献
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Objective:To study whether adenovirus-mediated human β-nerve growth factor (Ad-hNGFβ) gene has any protective effect on blast hearing impairment. Methods:Deafness was induced by blast exposure (172.0 dB) in 30 healthy guinea pigs. On day 7 of blast exposure, Ad-hNGFβ was infused into the perilymphatic space of 20 animals as the study group (hNGFβ group), and artificial perilymph fluid (APF) was infused into the perilymphatic space of the other 10 animals as the control group. At weeks 1, 4 and 8 after blast exposure, the animals were sacrificed and the cochleae were removed for immunohistochemical and HE stainings. Results: Expression of Ad-hNGFβ protein was detected in each turn of the cochlea at the 1st week, with almost equal intensity in all turns. At the 4th week, the reactive intensity of the expression of Ad-hNGFβ protein decreased. At the 8th week, no expression was detectable. The results of HE staining showed that the amount of spiral ganglions in hNGFβ group was significantly greater than that of the control group at week 4 (P〈0.01). Conclusion: Ad-hNGFβ can be expressed at a high level and for a relatively long period in the blast impaired cochlea, suggesting that Ad-hNGFβ has a protective effect on cochlear spiral ganglion cells after blast exposure and the efficient gene transfer into cochlea had been achieved without toxicity. 相似文献
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目的 为解除咽缩肌的痉挛 ,提高喉全切除术后用发音管发音重建的成功率。方法 在一侧舌骨大角后内侧咽缩肌的后表面 6 5mm左右 ,可找到咽丛神经支配咽下缩肌的主支或分支 ,将其切断。结果 16例中 15例咽丛神经切断术后发音重建成功 ,成功率为 93 8%。结论 咽丛神经切断术取代咽缩肌切断术是可行的。咽丛神经切断术能提高Blom Singer发音管发音重建的成功率 ,值得推广应用。 相似文献
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微矩阵基因芯片筛选喉鳞癌相关基因的研究 总被引:11,自引:1,他引:10
目的:应用基因微矩阵方法筛选喉鳞癌相关基因。方法:按一步法抽提喉鳞癌和对照喉正常组织的总DNA并纯化mRNA;将4096种人类基因PCR产物用CartesianPixsys7500点样仪按微矩阵排列点样于化学涂层的载玻片上,制成基因芯片;将等量的对照组织和喉鳞癌组织mRNA分别逆转录合成荧光分子掺入的cNDA-链做探针,混合后杂交上述基因芯片,经严格洗片后用ScanArray3000扫描仪扫描芯片 相似文献
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喉癌中人乳头状瘤病毒感染的检测及其临床分析 总被引:1,自引:0,他引:1
目的:探讨喉鳞状细胞癌中人乳头状瘤病毒(HPV16,18亚型)感染情况及临床分析。方法:用免疫组化的方法对42例喉鳞癌组织中HPV16,18亚型情况进行检测。结果:42例喉鳞癌组织中HPV16,18亚型感染阳性率64.29%(27/42),但HPV16,18感染与临床分期、癌细胞的分化程度、肿瘤的淋巴结转移和临床分型无关。结论:HPV16,18感染可能是人喉癌形成的触发因素,它对肿瘤生物学行为的影 相似文献
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鼻咽癌由于鼻咽癌部位隐蔽,症状比较复杂,如单侧头痛或复视、上颈部肿块,后吸性涕中带血,一侧分泌性中耳炎等,常可作为早期症状或首发症状单独出现,因此容易漏诊、误诊。此外,慢性上颌窦炎及慢性蝶窦炎亦可能是鼻咽癌患者的首发症状或表现。现将我科近年来收治的3例以慢性蝶窦炎为首发表现的鼻咽癌报告如下。 相似文献
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目的:评价富马酸卢帕他定片治疗过敏性鼻炎临床有效性和安全性试验设计:随机、双盲、阳性药平行对照.方法:将入选的过敏性鼻炎患者144例:随机分为试验组72例,对照组72例.试验药:富马酸卢帕他定片,每次1片,每日1次,对照药:盐酸西替利嗪片,每次1片,每日1次,观察周期14天.疗效性指标:过敏性鼻炎症状、体征.安全性指标:一般体格检查,血尿常规,肝肾功能,心电图,不良事件.研究结果:分析结果:两组病人用药后2周临床疗效等级的比较差异无统计学意义(P=0.8303);临控率对照组66.20%,试验组64.79%,两组间比较差异无统计学意义(P=0.8906).有效率对照组91.55%,试验组90.14%,两组间比较差异无统计学意义(P=0.817 3).安全性分析:两组病人用药后不良反应情况:不良反应发生率对照组5.63%,试验组2.82%,两组间比较差异均无统计学意义.结论:富马酸卢帕他定片治疗过敏性鼻炎安全、有效. 相似文献