Cultispher微载体构建组织工程软骨修复关节软骨缺损北大核心

背景:纤维蛋白胶和微载体均可作为软骨组织工程的良好载体,但因力学性能差和可塑性差等缺点限制其广泛应用。目的:以负载软骨细胞的Cultispher微载体为基础,复合纤维蛋白胶,构建Cultispher微载体/纤维蛋白胶复合支架,观察其用于修复兔膝关节软骨缺损的效果。方法:将兔软骨细胞与Cultispher微载体置于搅拌式生物反应器中三维悬浮共培养,待细胞帖附至微载体表面并大量扩增后,将负载有软骨细胞的Cultispher微载体与纤维蛋白胶复合,构建Cultispher微载体/纤维蛋白胶复合支架,并用于修复兔膝关节股骨滑车软骨缺损。实验按不同的软骨缺损处植入物分为3组:MCF组以负载软骨细胞的Cultispher微载体/纤维蛋白胶复合支架修复软骨缺损;MF组以单纯Cultispher微载体/纤维蛋白胶复合支架修复软骨缺损;空白对照组旷置软骨缺损,不作任何处理。术后3,6个月取材。检测并记录大体观、大体观评分、病理学染色、病理学评分、Micro-CT扫描等指标,评估软骨修复效果。结果与结论:①大体观显示MCF组的软骨修复效果明显优于MF组和空白对照组;②番红"O"、天狼猩红病理染色结果显示MCF组的修复组织主要以透明软骨为主,而MF组和空白对照组的修复组织主要以纤维组织为主;③Micro-CT扫描结果显示,MCF组较MF组和空白对照组获得更好的软骨下骨重建;④MCF组的大体观评分与病理学评分均明显高于MF组和空白对照组;⑤结果证实,负载软骨细胞的Cultispher微载体/纤维蛋白胶复合支架能成功修复兔股骨滑车软骨缺损。     

细胞外基质来源支架在软骨组织工程中的应用

BACKGROUND: At present, a variety of extracellular matrix-derived scaffolds have been successfully applied for cartilage tissue engineering in experiment and clinical practice. OBJECTIVE: To summarize the application and research status of extracellular matrix-derived scaffolds in cartilage tissue engineering. METHODS: A computer-based online search in PubMed, CNKI, CqVip and WanFang databases was performed using the keywords of “tissue engineering, cartilage, extracellular matrix, scaffolds” in English and Chinese, respectively. A total of 1 140 literatures were retrieved, and finally 65 eligible literatures were included. RESULTS AND CONCLUSION: In terms of the components, extracellular matrix-derived scaffolds are divided into monomeric natural polymers, mixed natural polymers, natural polymers compositing with synthetic polymers as well as acellular extracellular matrix-derived materials. Extracellular matrix-derived scaffolds hold good biocompatibility and degradability, and can promote proliferation and differentiation of choncrodytes; therefore, they as good bionic scaffolds have been applied for cartilage tissue engineering in clinical practice, However, poor mechanical properties and difficulty to molding should never be ignored. Further research should focus on improving the preparation technology by combining synthetic materials with extracellular matrix-derived scaffolds for cartilage tissue engineering.