成熟瘢痕组织与正常皮肤脱细胞真皮基质的生物学性能比较

BACKGROUND: It is necessary to carry out multiple operations to remove the scar in patients with large area of scar, and whether the scar tissue can be recycled has become the focus of the study. OBJECTIVE: To compare the tissue structure, biomechanical properties and biocompatibility of the acellular dermal matrix of mature scar tissue and normal skin. METHODS: The acellular dermal matrix was prepared from the human mature scar tissue and the normal skin around the scar. Subsequently, histological and scanning electron microscope observations were performed, and biomechanical properties were detected using universal tensile testing machine. Then, the acellular dermal matrix from mature scar tissue and normal skin was co-cultured with fibroblasts for 10 days, respectively, and the cell growth curve was drawn. Additionally, the acellular dermal matrix from mature scar tissue and normal skin was subcutaneously implanted into the dorsal tissue of Sprague-Dawley rats, respectively and histological observation was conducted at 4, 8 and 12 weeks after implantation. RESULTS AND CONCLUSION: There were many gaps but no cellular components in the acellular dermal matrix, in both two groups. Collagen fibers of the acellular dermal matrix derived from mature scar were looser than that of the normal skin, and arranged slightly irregularly; the biomechanical properties of the acellular dermal matrix derived from mature scar were similar to that of the normal skin, which exhibited appropriate flexibility and strength. There was no significant difference in the growth state of the two kinds of acellular dermal matrix, and the growth curve was basically consistent. After 4 weeks of implantation, more inflammatory cells infiltration could be found in the mature scar group, and in contrast, only a few inflammatory cells infiltration appeared in the normal skin group, These inflammatory reactions disappeared with time in both two groups. Besides, collagen fibers arranged in neat, and small vessels grew into the implants in both two groups. In conclusion, the tissue structure, biomechanical properties and biocompatibility of the acellular dermal matrix derived from scar tissue are almost consistent with those of the human normal skin.     

面部皮肤恶性肿瘤外科手术后缺损修复38例

目的:探讨面部皮肤恶性肿瘤的手术切除后缺损修复方法及其效果。方法:对38例面部肿瘤患者进行肿瘤扩大切除术,采用原位缝合、皮片移植或局部皮瓣转移修复缺损。结果:38例中原位缝合11例,植皮13例,局部皮瓣转移14例。除1例皮瓣远端表皮坏死给予换药外,切口均愈合良好。结论:在面部肿瘤的手术治疗中应兼顾外形的恢复,推荐使用全厚皮片、局部皮瓣修复皮肤缺损。     

孩子为什么不乖乖睡觉

儿童睡眠障碍的表现夜间频频醒来,睡不安稳、恐惧黑暗、夜间磨牙、遗尿、呓语、梦游、睡觉中大哭、摇动身体、抓挠皮肤、入睡困难和易惊醒等等。儿童睡眠障碍原因归纳起来有以下几种情况:首先是精神刺激,比如受惊吓,或有苦恼又不愿让家长知道,或家庭关系紧张,总在压抑中过日子等。其次是疾病,最常见的是特异性皮肤病,其中90%的儿童年     

吉林市高校大学生健康素养及其影响因素分析

了解吉林市高校大学生健康素养现状及其影响因素,为提高大学生的健康素养水平提供参考.方法 以2008年卫生部发布的《中国公民健康素养:基本知识与技能》的内容为基础自行设计调查问卷,对4所高校分层随机抽取的1 700名大学生进行调查.结果 大学生总体健康素养水平为10.82%,健康基本知识、健康生活方式与行为和健康技能3个维度素养具备率分别为10.00%,18.53%和53.94%.医学生健康素养及其各维度具备率均高于其他类型院校,差异均有统计学意义(P值均<0.01).女生健康素养具备率高于男生;大三学生好于大一和大二学生(P值均<0.05).多因素Logistic回归分析显示,不同类型院校和年级是影响大学生健康素养水平的主要因素(P值均<0.05).结论 大学生健康素养总体水平一般.高校应当注重大学生健康素养的教育,提高大学生健康素养水平.     

手术治疗颜面部皮肤癌的临床疗效观察

目的探讨手术治疗颜面部皮肤癌的临床治疗方法与效果。方法回顾分析淮北市人民医院2009年12月-2011年12月期间收治的38例颜面部皮肤恶性肿瘤患者的临床资料，并对手术方式及手术的治疗效果等进行总结。结果颜面部皮肤癌的手术治疗方式异常复杂，在手术的治疗之后，所有患者的皮瓣均成活。皮片有1例出现了表皮坏死的情况，其余的全部成活，且患者切口的愈合情况良好，在之后的随访中，患者的术区及功能等恢复情况均良好。结论运用外科手术的先进技术来治疗颜面部皮肤癌患者不仅非常的安全和可靠，且具有相当显著的临床治疗效果。     

高效液相色谱法同时测定古方生化汤中阿魏酸和川芎嗪的含量

目的:建立用反相高效液相色谱法同时测定古方生化汤中阿魏酸和川芎嗪含量的方法.方法:以甲醇-水(含1%的冰醋酸),(7:18)为流动相,Zorbax C18柱(250 mm×4.6 mm,5 μm)为固定相,紫外检测波长为280 nm,外标法定量.结果:阿魏酸和川芎嗪分离完全,阿魏酸在8.06～64.48 mg·L-1的浓度范围内回归,方程为Y=1811.32 1170.33X,r=0.9954,平均回收率为97.9%,RSD为1.4%;川芎嗪在4.03～20.15 mg·L-1的浓度范围内,回归方程为Y=16.60 1401.94X,r=0.9941,平均回收率为101.9%,RSD为2.6%.结论:此法快捷、简便,准确,可作为生化汤的质量标准.