兔脑 —氨基丁酸结合蛋白（受体）在非洲瓜蟾卵母细胞上的重建

Gurdon's Xenopus laevis oocyte translation system has been extensively employed to investigate the molecular biology of gamma-aminobutyric acid (GABA) receptors in the brains of embryonic chick, rat, etc. As GABA and its receptor may play a role in the pathogenesis of hepatic encephalopathy and a rabbit model of fulminant hepatitis has been established, we reconstituted the rabbit model of fulminant hepatitis has been established, we reconstituted the rabbit brain GABA binding protein (receptor) on Xenopus laevis oocytes to offer a base for further study of the pathological effects of GABA receptor at molecular level. In this report, total RNA was extracted from rabbit brain by guanidine hydrochloride method. The total RNA was then subjected to oligo-(dT)-cellulose affinity chromatography to isolate mRNA. All the RNA samples achieved a high purity with an average OD260/OD280 ratio of about 2.10. The clear bands of 18 S and 28S rRNA in electrophoresis implied that the RNA was not degraded and was therefore available for expression. After two days of incubation of the oocytes injected with mRNA, radioreceptor assay indicated that saturable and specifically displaceable GABA binding sites were implanted onto the oocyte membrane. This result led us to the conclusion that rabbit brain GABA binding protein (receptor) can be reconstituted by injecting exogenous mRNA inclusive of those encoding the receptor into Xenopus oocytes. However, the functional activity of the reconstituted receptor as a GABA gated chloride ion channel needs to be further characterized by patch clamping.     

黄疸出血群赖型钩端螺旋体“TM抗原”的提取和初步鉴定

本文报道用90％酚、乙醇沉淀法提取黄疸出血群赖型017株“TM抗原”。用双向琼脂扩散法及补体结合试验探讨其型特异性,对其理化性质作了红外光谱分析及电镜观察。     

生大黄在治疗暴泼性肝衰型肝性脑病中的效果及其机理的实验探讨(摘要)

作者在腹腔注射D—氨基半乳糖(GalN) 于Wistar雄性大鼠复制的暴发性肝衰型肝性脑病模型上用生大黄煎液灌胃治疗,观察疗效并用放射受体分析法动态测定了动物血浆GABA浓度变化。结果表明:肝衰组动物,在GalN注射后,随病情加重,血中GABA随之增高,二     

大鼠暴发性肝功能衰竭型肝性脑病血浆γ-氨基丁酸浓度变化的实验研究

作者采用放射受体分析法,测定D-氨基半乳糖(GalN)复制的暴发性肝衰大鼠模型其血浆γ-氨基丁酸(GABA)浓度的动态变化。结果表明:注射GalN后,随大鼠病情加重,GABA浓度随之增高,二者呈平行关系(r=0.9443,P<0.05)。48小时出现肝性脑病时,GABA浓度达到高峰,为注射前8倍。提示:肝衰动物血浆GABA浓度增高与肝性脑病发病有关。     

兔脑γ-氨基丁酸结合蛋白(受体)在非洲爪蟾卵母细胞上的重建

作者采用盐酸胍法提取兔脑RNA,并经寡聚-(dT)-纤维素亲和层析分离获得完好的总mRNA,这种异源mRNA注入非洲爪蟾卵母细胞胞浆后经放射受体分析证实,γ-氨基丁酸结合蛋白(受体)能被重建于细胞膜上。该重建技术稳定可靠,为进一步进行γ-氨基丁酸受体的体外研究奠定了基础。     

兔脑γ-氨基丁酸结合蛋白(受体)在非洲爪蟾卵母细胞上的重建

非洲爪蟾卵母细胞翻译系统已被广泛用于胚鸡、大白鼠等动物脑GABA受体的分子生物学研究。鉴于GABA及其受体在肝性脑病中的作用和暴发性肝炎兔模型的建立,我们在卵母细胞上重建了兔脑GABA结合     

γ-氨基丁酸在肝性脑病发病机理中的作用

近年,国外学者在暴发性肝衰型脑动物模型用放射免病分析测定脑组织神经递质受体功能的改变‘结果表明:兴奋性氨基酸神经递质(天门冬氨酸、谷氨酸)受体密度减少;抑制性氨基酸神经递质(甘氨酸、γ-氨基丁酸)受体密度增加。而毒覃硷能受体和鸦片受体功能无改变。目前研究表明:1)脑内γ-氨基丁酸水平增加与血中γ-氨基丁酸水平增加有关。2)     

生大黄在治疗暴发性肝衰型肝性脑病中的效果及机理的初步探讨

作者采用腹腔注射专一性肝毒性药物——D-氨基半乳糖(GalN)于Wistar雄性大鼠复制暴发性肝衰型肝性脑病模型,用放射受体分析法动态测定了血中GABA浓度变化,并用生大黄煎液灌胃治疗肝衰动物。实验结果表明;肝衰组动物,在GalN注射后,随病情加重,血中GABA随之增高,二者呈平行关系     

应用ELISA检测SLE病人血清抗双链DNA抗体

作者用ELISA间接法检测SLE、非SLE病人及正常人血清抗双链DNA抗体。结果显示:活动期SLE病人19例中18例阳性;非活动期SLE病人32例、非SLE病人11例及正常人48例均为阴性。本法用于鉴别活动期SLE具有高度特异性和敏感性,操作简便,结果可靠,重复性好。