探讨舒适护理在股骨颈骨折护理中的应用

目的 探讨舒适护理在股骨颈骨折护理中的应用.方法 收集2011年6月--2012年9月来本院就诊,临床诊断为股骨颈骨折需要手术的患者90例,根据患者在院期间所接受的护理方式分为A、B两组,A组患者接受一般常规护理,B组患者接受舒适护理,通过对90例股骨颈骨折术后患者的对症护理效果,探讨舒适护理在股骨颈骨折护理中的应用.结果 对A、B两组股骨颈骨折术后患者的对症护理效果进行统计分析,差异有统计学意义,P＜0.05.结论 通过对老年骨折术后患者实施舒适护理,可降低术后并发症的发生率,提高治愈率,更易让患者满意.     

新型壳聚糖基仿生网络复合膜诱导骨髓间充质干细胞定向成骨分化

Objective To study the osteogenic differentiation capacity of mesenchymal stem cells(MSCs)on chitosan based biomimetic network composites films.Methods The chitosan-based biomimetic network composites films were prepared using chitosan,gelatin and pectin in certain ratio by biomimetic approach.The experiment was designed as follow:experimental group 1 (composites films+routine medium),control group 1 (routine medium),experimental group 2(composites films+osteogenic medium),control group 2(osteogenic medium).The growth and proliferation of MSCs were observed by inverted phase contrast microscope and scanning electronmicroscopy(SEM) and detected through methyl thiazolyl tetrazolium(MTT) assay.Alkaline phosphatase(ALP) activity was used to investigate the osteogenic differentiation capacity.The formation of mineral was determined by special staining and the energy dispersive X-ray apparatus (EDX).Results MSCs could well adhere,grow and proliferate on the network composites films.The MTT assay demonstrated that there was no significant difference in cell viability between the experimental and control groups (P＞0.05).SEM observation showed that MSCs grew assembly on the surface of the films secreted a large number of extracelluar matrix,and formed scattered nodules.The cells on the experimental group 1 showed statistically higher ALP activity level than those cells on the other groups (P＜0.01).Moreover,the calcified nodules can be seen in the experimental groups by Alizarin red and Von Kossa staining.ALP staining showed blue-staining granules within the cytoplasm,and the deposition of Ca and P was detected on the films by SEM-EDX.Conclusion The novel chitosan-based biomimetic network composites films not only have good biocompatibility,but can also improve MSCs differentiation toward the osteogenic lineage.     

MC3T3-E1细胞与多组分纳米羟基磷灰石基三维复合支架材料的相容性

目的 观察MC3T3-E1细胞在复合支架材料上的黏附、增殖及形态,评价多组分纳米羟基磷灰石基三维复合支架材料的生物相容性.方法 采用仿生学方法,将壳聚糖、羟基磷灰石、明胶、果胶按照一定比例制作成多组分纳米羟基磷灰石基三维复合支架材料.在复合支架材料上接种MC3T3-E1细胞,通过倒置相差显微镜、HE染色、扫描电镜、四甲...     

新型壳聚糖基仿生支架材料生物相容性的评价

目的: 评价新型壳聚糖基仿生支架材料的生物相容性.方法: 采用仿生学方法,将壳聚糖、明胶、果胶按一定比例制成新型壳聚糖基仿生支架材料.体外培养骨髓间充质干细胞(MSCs)并与支架材料复合培养,通过倒置相差显微镜、H-E染色、扫描电镜、四甲基偶氮唑盐(MTT)法检测细胞的生物相容性.结果: 新型壳聚糖基仿生支架材料呈半透明薄膜状.MSCs接种在支架材料上,倒置相差显微镜观察到细胞在材料上贴附、生长良好、增殖旺盛;H-E染色显示细胞胞质丰富,核卵圆形,可见1～2个清晰的核仁;扫描电镜可见细胞伸出多个伪足样突起,互相连接,并分泌大量细胞外基质;MTT法检测细胞活性显示仿生材料组吸光度值与对照组比较差异无统计学意义.结论: 新型壳聚糖基仿生支架材料蕴涵丰富的生物信息,细胞毒性小,生物相容性好,是一种很好的组织工程支架材料.     

新型壳聚糖基仿生网络复合膜诱导骨髓间充质干细胞定向成骨分化

目的 探讨新型壳聚糖基网络复合膜诱导骨髓间充质干细胞（MSCs）定向成骨分化的可行性.方法 采用仿生学方法,壳聚糖、明胶、果胶按照一定比例制作成新型壳聚糖基仿生网络复合膜.设计4个组：实验组1（复合膜＋常规培养基）,对照组1（常规培养基）,实验组2（复合膜＋成骨诱导（OS）培养基）,对照组2（OS培养基）.通过倒置相差显微镜、四甲基偶氮唑盐（MTT）法、扫描电镜（SEM）检测细胞在复合膜上的生长和增殖情况;通过测定碱性磷酸酶（ALP）活性来评价MSCs在复合膜上的成骨分化能力;通过特殊染色和能谱分析（EDX）来评定钙盐的沉积.结果 MSCs在网络复合膜上贴附、生长良好且增殖旺盛.MTr法检测细胞活力显示实验组吸光度（OD）值与对照组比较无统计学意义（P＞0.05）.SEM观察到细胞在支架材料表面呈聚集生长,分泌大量的细胞外基质,可见散在的结节形成.实验组1的ALP活性明显增高,与实验组2、对照组比较有统计学意义（P＜0.01）.茜素红和Von Kossa染色可见实验组细胞分化后形成的钙化结节;ALP染色可见胞浆内蓝染颗粒;EDX检测到Ca、P沉积.结论 新型壳聚糖基网络复合材料具有良好的生物相容性,在不添加诱导剂的条件下,可以诱导MSCs定向成骨分化.