小儿骨髓间质干细胞体外诱导分化为神经元样细胞

BACKGROUND:In the past, the culture and differentiation of bone marrow mesenchymal stem cells in vitro were mostly reported in the adult or animal rather than in children. OBJECTIVE:To explore the ability of bone marrow mesenchymal stem cells from children differentiating into neural stem cells and nerve cells. METHODS:Bone marrow mesenchymal stem cells from children were isolated and cultured, and passage 12 cells were cultured in the pre-induction medium (DMEM culture medium containing 10% fetal bovine serum and 1 mmol/L β-mercapto ethanol) and induction medium (DMEM containing 2% dimethyl sulfoxide and 150 μmol/L butylated hydroxyanisole). Expression of nestin and β-tublin III was detected using immunocytochemistry method at 30 minutes and 7 days after induction, while RT-PCR was used to detect nestin mRNA expression at 0, 5.5, 6 days after induction. RESULTS AND CONCLUSION: After combined induction, the cells shrank from round shape to tapered, polygonal or oval shape, and cell processes extended gradually and became filament-like shape. Interconnected cells formed a network at 6 days after combined induction. The expression of nestin antigen was positive at 30 minutes after induction, while the expression of β-tublin was positive at 7 days. RT-PCR findings showed that positive expression of nestin mRNA was detected at 5.5 hours of induction, and then disappeared at 6 days. These findings show that the combined use of dimethyl sulfoxide and butylated hydroxyanisole can induce bone marrow mesenchymal stem cells from children to differentiate into neural stem cells and nerve cells in vitro.     

儿童过敏性紫癜肾炎KIM-1和IL-18水平及其与EB病毒感染的关系

目的 探究儿童过敏性紫癜肾炎(HSPN)肾损伤分子-1(KIM-1)、白细胞介素-18(IL-18)水平及其与EB病毒(EBV)的关系。方法 选取2019年1月-2022年12月河南大学淮河医院收治的238例过敏性紫癜(HSP)患儿为研究对象,根据HSPN情况分为HSPN组(n=96)和非HSPN组(n=142),比较两组肾功能指标[血肌酐(Scr)、尿素氮(BUN)、胱抑素C(Cys-C)、24 h尿蛋白定量]、免疫学指标[免疫球蛋白A(IgA)、补体3、补体4]、EBV拷贝数及尿液KIM-1、IL-18表达水平;根据HSPN患儿外周血EBV-DNA拷贝数,可分为EBV阳性组(n=37)和EBV阴性组(n=59),比较两组肾功能指标、免疫学指标及尿液KIM-1、IL-18水平;分析EBV DNA拷贝数及尿液KIM-1、IL-18与HSPN患儿肾小球病理分级关系。结果 HSPN组皮疹持续时间较非HSPN组长(P<0.05),Scr、BUN、Cys-C、24 h尿蛋白定量、IgA、EBV DNA拷贝数、KIM-1、IL-18均较非HSPN组升高(P<0.05),补体3较非HS...     

儿童社区获得性肺炎病原菌及其疗效的影响因素

目的 探讨儿童社区获得性肺炎(CAP)病原菌与气道炎症因子及疗效的影响因素。方法 选取2019年6月-2021年9月河南大学淮河医院确诊的137例痰培养阳性CAP患儿，记录病原菌鉴定结果；根据患儿入院治疗7 d后临床疗效，分为有效组99例、无效组38例，比较两组患儿治疗前后气道炎症因子[诱导痰中核因子-κB(NF-κB)、半乳凝素3(Gal-3)、甲壳质酶蛋白40(YKL-40)]水平，分析气道炎症因子与病情程度[临床肺部感染评分(CPIS)、小儿危重病例评分(PCIS)]的相关性，探究影响疗效的风险因素及气道炎症因子对疗效的预测价值。结果 137例CAP患儿共培养分离细菌158株，其中革兰阴性菌92株占58.23%、革兰阳性菌66株占41.77%,以肺炎链球菌、流感嗜血杆菌、肺炎克雷伯菌为主；治疗前后，有效组NF-κB、Gal-3、YKL-40均低于无效组(P<0.05),且NF-κB、Gal-3、YKL-40治疗前后下降幅度大于无效组(P<0.05);多重细菌感染、CPIS评分、PCIS评分是CAP患儿抗菌疗效的影响因素(P<0.05);NF-κB、Gal-3、Y...