全唾液法分析糖尿病及牙周炎伴牙列缺损患者行种植义齿修复的临床效果

目的:探究糖尿病及牙周炎伴牙列缺损患者种植治疗的预后疗效,以此作为该类患者种植治疗术前评估,为提高种植成功率提供参考依据.方法:分别于种植前和种植后4周、12周与24周收集2型糖尿病患者组、牙周炎患者组以及对照组患者唾液,记录探诊深度、测量种植体稳定性,实验ELISA法检测唾液中的IL-1β与TNF-α,荧光定量PCR...     

3D打印羊椎骨粉/聚乙烯醇支架、纳米级羟基磷灰石/聚乙烯醇支架、羊椎骨粉/聚乙烯醇无孔骨板的性能比较

BACKGROUND: With the promotion of 3D printing technology, 3D printing scaffolds for bone tissue engineering have become the new ideas for jaw bone repair. OBJECTIVE: To compare the physical and biological properties of sheep vertebral bone meal/polyvinyl alcohol (PVA) scaffold, nano-hydroxyapatite (nHA)/PVA scaffold, and sheep vertebral bone meal/PVA nonporous bone plate. METHODS: 3D printing technology was used to print sheep vertebral bone meal/PVA scaffold, nHA/PVA scaffold, and sheep vertebral bone meal/PVA nonporous bone plate. Porosity, morphology, water absorption rate and mechanical properties of different scaffolds were detected. Three kinds of scaffolds were all used to culture bone marrow mesenchymal stem cells, and cell proliferation ability was detected using cell counting kit-8 at 1, 4, 7 days of culture. RESULTS AND CONCLUSION: Under scanning electron microscope, the sheep vertebral bone meal/PVA scaffold and nHA/PVA scaffold exhibited regular and interconnected pores with good continuity and clear network structure; the sheep vertebral bone meal/PVA nonporous bone plate had no obvious pores; however, it had dense and evenly distributed micropores with different sizes on its surface. The porosity of nHA/PVA scaffold was lower than that of the sheep vertebral bone meal/PVA scaffold (P < 0.05). The water absorption rate was highest for the nHA/PVA scaffold followed by the sheep vertebral bone meal/PVA scaffold and the sheep vertebral bone meal/PVA nonporous bone plate (P < 0.05). In contrast, the scaffold toughness was highest for the sheep vertebral bone meal/PVA nonporous bone plate, followed by the sheep vertebral bone meal/PVA scaffold and nHA/PVA scaffold. In addition, the cell proliferation activity of cells cultured on the sheep vertebral bone meal/PVA scaffold was significantly higher than that cultured on the other two kinds of scaffolds. Taken together, the 3D printing sheep vertebral bone/PVA scaffold has good physical and chemical performance.