排序方式: 共有65条查询结果,搜索用时 13 毫秒
1.
Objective To explore the effect of L-canosine in preventing and treating rat cataract induced by sodium selenite. Methods This was an experimental study. Cataract was induced in the rats by sodium selenite. Rats were divided into 3 groups and each group has 3 ones. L-canosine eye drops (50 g/L or 20 g/L) and 0.9% normal saline were respectively instilled to the rat eye for 3 weeks and examined. L-canosine was added to the medium of cultured rat cataract lens at 1.00, 0.10 and 0.01 g/L for 1 week and then examined. Cataract lens were studied by using two-dimensional eletrophoresis. Results One week after instillation of L-canosine, the scores of rat cataract were 2.22±0.65, 2.39±0.98 and 2.83±0.38 in 50 g/L, 20 g/L L-canosine and control groups, respectively. The lesion in 50 g/l, L-canosine group was lighter than that of the control group. There was significant difference between these two groups (P=0.013). On the 2nd and 3rd weeks, there was no difference among these three groups. After 1 week for culturing cataract lens, there was no difference between these three groups. The results of two-dimensional eletrophoresis showed that the protein number was 182 and 161 in the L-carnosine and control groups, respectively. High molecular weight protein decreased and low molecular weight protein increased in L-carnosine group. Conclusions L-carnosine at 50 g/L could restrain the development of early stage rat cataract. L-camosine could modulate the rat lens protein in vitro, but could not affect lens cataract scores. 相似文献
2.
3.
碱性成纤维细胞生长因子在人晶体上皮细胞的蛋白检测研究 总被引:5,自引:0,他引:5
研究成纤维细胞生长因子(basicfibroblastgrowthfactor,bFGF)对人晶体上皮细胞(humanlensepithelialcels,HLECs)的促增殖作用。方法:采用免疫组织化学检查(ABC法)来检测人晶体上皮细胞上bFGF蛋白水平,并用图像分析进行相对定量。结果:定性及定量地证明了人晶体上皮细胞有bFGF蛋白存在。结论:阐明了人晶体上皮细胞本身存在的bFGF以蛋白的形式参与了术后后囊混浊的形成。 相似文献
4.
生长因子与晶体后囊混浊 总被引:2,自引:0,他引:2
随着人工晶体植入术的普遍开展,其远期晶体后囊混浊的研究日益被人们重视。但对生长因子与晶体上皮细胞的关系认识不足。生长因子是一类多功能的分子信号多肽或蛋白质。正常人眼视网膜、玻璃体、房水、晶体上皮细胞等存在数种生长因子。有刺激增殖性及抑制性因子。它们之间需达到平衡状态,否则会引起各种病理改变。本文叙述生长因子的特点,晶体后囊混浊的病理生理及诱因,生长因子与晶体后囊混浊的关系及处理后囊混浊的研究等情况。 相似文献
5.
目的探讨改良法经第二三腰椎间隙行腰麻硬膜外联合阻滞(腰硬联合阻滞)对腰麻针置入即刻出现神经刺激症状的发生率和硬膜外导管置入蛛网膜下腔可能性的影响。方法ASAI或Ⅱ级拟行全子宫切除术、经第三四腰椎间隙(L3,4)行腰一硬联合阻滞穿刺失败的患者100例,均改为经第二三腰椎间隙(L2,)进行穿刺。根据穿刺手法不同,随机分为2组:传统方法组(C组)和改良法组(G组)。观察两组腰麻针置入蛛网膜下腔即刻神经刺激症状的发生率和硬膜外导管置入蛛网膜下腔的发生率。结果与C组相比,G组腰麻针置入蛛网膜下腔即刻出现神经刺激症状的发生率和硬膜外导管置入蛛网膜下腔的发生率均降低(P〈0.05),但两组患者术后均未出现任何症状。结论改良法经第二三腰椎间隙行腰硬联合阻滞可降低在该间隙穿刺腰麻针置入蛛网膜下腔即刻出现神经刺激症状的发生率和硬膜外导管置入蛛网膜下腔的发生率,从而可避免脊髓圆锥的损伤。 相似文献
6.
Objective To explore the effect of L-canosine in preventing and treating rat cataract induced by sodium selenite. Methods This was an experimental study. Cataract was induced in the rats by sodium selenite. Rats were divided into 3 groups and each group has 3 ones. L-canosine eye drops (50 g/L or 20 g/L) and 0.9% normal saline were respectively instilled to the rat eye for 3 weeks and examined. L-canosine was added to the medium of cultured rat cataract lens at 1.00, 0.10 and 0.01 g/L for 1 week and then examined. Cataract lens were studied by using two-dimensional eletrophoresis. Results One week after instillation of L-canosine, the scores of rat cataract were 2.22±0.65, 2.39±0.98 and 2.83±0.38 in 50 g/L, 20 g/L L-canosine and control groups, respectively. The lesion in 50 g/l, L-canosine group was lighter than that of the control group. There was significant difference between these two groups (P=0.013). On the 2nd and 3rd weeks, there was no difference among these three groups. After 1 week for culturing cataract lens, there was no difference between these three groups. The results of two-dimensional eletrophoresis showed that the protein number was 182 and 161 in the L-carnosine and control groups, respectively. High molecular weight protein decreased and low molecular weight protein increased in L-carnosine group. Conclusions L-carnosine at 50 g/L could restrain the development of early stage rat cataract. L-camosine could modulate the rat lens protein in vitro, but could not affect lens cataract scores. 相似文献
7.
Objective To explore the effect of L-canosine in preventing and treating rat cataract induced by sodium selenite. Methods This was an experimental study. Cataract was induced in the rats by sodium selenite. Rats were divided into 3 groups and each group has 3 ones. L-canosine eye drops (50 g/L or 20 g/L) and 0.9% normal saline were respectively instilled to the rat eye for 3 weeks and examined. L-canosine was added to the medium of cultured rat cataract lens at 1.00, 0.10 and 0.01 g/L for 1 week and then examined. Cataract lens were studied by using two-dimensional eletrophoresis. Results One week after instillation of L-canosine, the scores of rat cataract were 2.22±0.65, 2.39±0.98 and 2.83±0.38 in 50 g/L, 20 g/L L-canosine and control groups, respectively. The lesion in 50 g/l, L-canosine group was lighter than that of the control group. There was significant difference between these two groups (P=0.013). On the 2nd and 3rd weeks, there was no difference among these three groups. After 1 week for culturing cataract lens, there was no difference between these three groups. The results of two-dimensional eletrophoresis showed that the protein number was 182 and 161 in the L-carnosine and control groups, respectively. High molecular weight protein decreased and low molecular weight protein increased in L-carnosine group. Conclusions L-carnosine at 50 g/L could restrain the development of early stage rat cataract. L-camosine could modulate the rat lens protein in vitro, but could not affect lens cataract scores. 相似文献
8.
精氨酸甘氨酸天门冬氨酸序列(RGD)是整合素受体与细胞外配体相结合的识别位点。由于合成的RGD肽具有抑制细胞粘附的作用,所以对眼各种增殖性病变具有潜在的临床应用价值。本文综述了RGD的本质、生物学功能、作用机制和特点,以及RGD肽在眼科中的研究应用。 相似文献
9.
10.