归熟汤治疗腰椎间盘突出症218例疗效观察

腰椎间盘突出症是临床常见病 ,治疗上方法很多 ,但疗效不一。自 1998～ 2 0 0 2年 3月 ,作者先后收治手术、大推拿和腰椎牵引治疗无效的病例 2 18例 ,经辨证应用归熟汤加减治疗 ,取得满意疗效。现总结报告如下。1　临床资料本组 2 18例 ,男 12 4例 ,女 94例。年龄最小 17岁 ,最大 71岁 ,平均 4 2岁。本组曾用开窗手术治疗 6 8例 ,电视椎间盘镜治疗 2 8例 ,经皮切吸治疗 3例 ,大推拿治疗 86例 ,骨盆牵引 33例。在上述治疗后来诊者最长 2年 ,最短 (骨盆牵引后 ) 3天。据手术、大推拿和腰椎牵引前ＣＴ检查所示中央型突出 2 8例 ,中央偏左型 4 4…     

膝关节康复器的研制及其临床应用

下肢骨折,膝关节损伤引起关节装置骨折和韧带断裂,或膝关节术后都往往会并发膝关节僵直的功能障碍.如不及时认直进行康复治疗,就会留有不同程度的膝关节伸屈功能障碍,影响患者生活和工作.作者于1998年自行设计研制出一种膝关节康复器,运用自身的力量,进行膝关节功能康复治疗,经126例观察疗效满意.现总结报告如下.     

魏清琳治疗围绝经期功血经验

<正>魏清琳主任医师,硕士研究生导师,甘肃省名中医,甘肃针灸学会副会长,现任甘肃中医药大学附属医院针灸科主任。从事中医针灸临床、教学、科研工作26年,擅长运用针灸、中药、气功等传统医疗保健方法诊治各类疑难杂症,应用针灸综合技术(针刺、拔罐、艾灸、埋线、穴位注射、小针刀疗法、小儿推拿等方法),在治疗临床各种急慢性疾病方面积累较为丰富的临床经验,形成独特的诊疗思路,诊治过程中注重     

非发酵菌的临床分布与耐药性分析

目的研究非发酵菌的临床科室分布以及耐药性,为临床合理运用抗菌药物提供理论依据。方法回顾性分析医院2010-2012年细菌培养分离的非发酵菌,细菌分离培养按《全国临床检验操作规程》进行,采用法国生物梅里埃公司全自动微生物VITEK-2Compact分析仪鉴定到种,体外药敏试验采用纸片K-B法,采用WHONET 5.6软件进行统计分析。结果共分离出非发酵菌4 225株,前3位为鲍氏不动杆菌1 636株、铜绿假单胞菌1 537株、嗜麦芽寡养单胞菌426株,分别占38.7%、36.4%、10.1%;标本来源以呼吸道标本为主,占76.8%,其次为分泌物与脓液,分别占9.3%;分离菌株主要来自ICU占22.4%和儿科占18.0%;鲍氏不动杆菌对头孢西丁的耐药率最高为93.7%,对米诺环素的耐药率最低为18.0%,铜绿假单胞菌对头孢西丁、米诺环素的耐药率均为100.0%。结论非发酵菌在临床标本的分离率较高,耐药率也普遍较高,应加强耐药监测,从而为抗菌药物的合理使用及医院感染的有效控制提供理论依据。     

反义miR-21通过RECK抑制胶质瘤细胞侵袭性生长的体内外研究

目的 探讨胶质瘤细胞LN229中RECK作为miR-21的调控靶点,在胶质瘤侵袭性生长中的作用.方法 将反义miR-21(AS-miR-21)寡核苷酸转染至人脑胶质瘤细胞LN229中.Real-time PCR检测LN229细胞中miR-21的表达量.荧光素酶实验检测miR-21对RECK的调控关系.Transwell实验评价LN229细胞侵袭能力的变化,应用Western blot检测细胞内MMP2/9和RECK蛋白水平的变化,ELISA实验检测培养基中活性MMP2/9的表达量,动物实验评价体内条件下肿瘤侵袭性的变化.结果 Real-time PCR显示转染组中miR-21的表达量与对照组相比下调60%.荧光素酶实验证明RECK是miR-21的靶点.Transwell实验证实胶质瘤细胞侵袭能力下降,Western blot和ELISA实验证实MMP2/9表达降低,动物实验及免疫荧光反映肿瘤侵袭性生长受抑制.结论 反义miR-21通过上调RECK的表达而抑制恶性胶质瘤细胞的侵袭性生长.

Abstract：

Objective To investigate the regulation of miR - 21 on invasion growth of human glioma cells by RECK.Method The human glioma LN229 cells were transfected with AS - miR - 21 or scrambled sequences by Lipofectamine2000.Real time PCR was conducted to detect the expression of miR-21.Luciferase experiment was performed to detect the relationship between miR-21 and RECK.The expression of RECK was evaluated by Western blot.The invasion ability was evaluated by transwell assay and subcutaneous models.Western Blot, ELISA and immunofluorescence were used to estimate the changes of MMP2/9.Results The expression of miR - 21 in LN229 cells decreased after transfection with AS-miR-21. It was proved that RECK was a direct target of miR -21 by luciferase experiment.Meanwhile, the high expression of RECK protein in AS - miR -21 group conformed its important function in this mechanism.Transwell assay demonstrated decreased invasion capability of LN229 cell lines transfected with AS- miR- 21.Western blot, ELISA, and immunofluorescence demonstrated the levels of MMP2/9 were down -regulated in AS -miR -21 group compared with control and scrambled group.Conclusions AS - miR -21 could depress the invasion of glioma cells owing to up - regulating the level of RECK which could inhibit MMP2/9 activities both in vitro and vivo.     

肾俞穴与肾位置关系的影像学研究*

目的：应用磁共振影像技术,观察肾俞穴水平断面肾的显示情况,了解肾俞穴与肾的位置关系,为避免针刺损伤肾提供依据。方法：选择成年健康志愿者160例,用磁共振仪采集肾俞穴的水平断面影像,观察断面影像的肾显示情况,并进行统计分析。结果：160例肾俞穴水平断面影像中,未见双肾62例(38.75%),仅见右肾57例(35.62%),可见双肾35例(21.88%),仅见左肾6例(3.75%)。男女之间差异无统计学意义,体型正常、超重之间差异无统计学意义。结论：肾位于肾俞穴的外上方,在肾位置正常的情况下,肾下端未达肾俞穴水平断面。但肾位置存在解剖变异,且受体位、呼吸等因素的影响,肾下端达肾俞穴水平断面的情况较多。肾俞穴针刺时应谨慎,不宜深入腹腔,以避免损伤肾。肾俞穴向外上方深刺时,更易伤及肾。     

聚酰胺-氨纳米微粒介导5-氟尿嘧啶联合小RNA-21抑制乳腺癌细胞生长的研究

Objective To study the effect of PAMAM-mediated 5-fluorouracil combined with miR-21 inhibitor gene therapy to suppress MCF-7 human breast cancer cell growth in vitro. Methods 5-Fu/PAMAM complex was prepared by dialysis method and then incubated with miR-21 inhibitor at room temperature. Transmission electronic microscopy (TEM) was performed to observe the morphology of the nanoparticles. The drug loading efficiency and encapsulation efficiency was determined by ultraviolet spectroscopy (UV). The transfection of PAMAM dendrimer was detected by flow cytometry. MTT assay was carried out to determine MCF-7 cell growth survival rate. Cell apoptosis was analyzed by flow-cytometry. Transwell assay was performed to detect invasion ability after MCF-7 cells treated with 5-Fu chemotherapy combined with miR-21 inhibitor gene therapy. Results The morphology of the complex was sphere observed under TEM. Encapsulation efficiency and loading efficiency of drug were (66. 21±4. 11)% and (31.77±0. 73)% , respectively. Flow cytometry revealed that 5-Fu/PAMAM transfection efficiency was (60.54 ±6. 97)%. 5-Fu combined with miR-21 inhibitor treatment significantly suppressed cell growth, and the survival rate was only (55. 85±3. 71)% on the 6th day of the observation period. The apoptosis rate in combined treatment group was (18. 32±2.42)% , dramatically higher than in control group (F=58. 326,P<0. 01). In combined treatment group, the number of invasion cells was only 18. 96 ±3. 14, suggesting the greatly decreased invasion ability of MCF-7 cells (F=16. 409,P < 0. 01). Conclusion PAMAM could effectively deliver miR-21 inhibitor and 5-Fu simultaneously, and combined therapy can suppress growth of MCF-7 cells effectively in vitro.