排序方式: 共有24条查询结果,搜索用时 31 毫秒
1.
Miterpáková Martina Valentová Dana Čabanová Viktória Berešíková Ľudmila 《Parasitology research》2018,117(7):2347-2350
Parasitology Research - Until recently Dirofilaria immitis, the causative agent of serious canine heartworm disease, has been detected relatively infrequently in Central Europe in comparison with... 相似文献
2.
Sleep and Breathing - Obstructive sleep apnea (OSA) is associated with oxidative stress that is involved in the pathogenesis of cardiovascular and metabolic complications. The concentrations of... 相似文献
3.
4.
Andrea Kucharíková Andrea Schreiberová Monika Závodská Štefánia Gedrová Ľudmila Hricová Jaroslav Pavel Ján Gálik Martin Maršala Nadežda Lukáčová 《Acta histochemica》2014
The interruption of supraspinal input to the spinal cord leads to motor dysfunction and the development of spasticity. Clinical studies have shown that Baclofen (a GABAB agonist), while effective in modulating spasticity is associated with side-effects and the development of tolerance. The aim of the present study was to assess if discontinued Baclofen treatment and its repeated application leads antispasticity effects, and whether such changes affect neuronal nitric oxide synthase (nNOS) in the brainstem, nNOS and parvalbumin (PV) in lumbar α-motoneurons and glial fibrillary acidic protein in the ventral horn of the spinal cord. Adult male Wistar rats were exposed to Th9 spinal cord transection. Baclofen (30 mg/b.w.) diluted in drinking water, was administered for 6 days, starting at week 1 after injury and then repeated till week 4 after injury. The behavior of the animals was tested (tail-flick test, BBB locomotor score) from 1 to 8 weeks. Our results clearly indicate the role of nitric oxide, produced by nNOS in the initiation and the maintenance of spasticity states 1, 6 and 8 weeks after spinal trauma. A considerable decrease of nNOS staining after Baclofen treatment correlates with improvement of motor dysfunction. The findings also show that parvalbumin and astrocytes participate in the regulation of ion concentrations in the sub-acute phase after the injury. 相似文献
5.
Relationship between A163G osteoprotegerin gene polymorphism and other osteoporosis parameters in Roma and non‐Roma postmenopausal women in eastern Slovakia
下载免费PDF全文
![点击此处可从《Journal of clinical laboratory analysis》网站下载免费的PDF全文](/ch/ext_images/free.gif)
6.
7.
8.
Alexandra Gal Koval
íkov Alexandra Pan
íkov Barbora Kone
n Tatiana Klamrov Bohuslav Novk Eva Kovaov
udmila Podrack Peter Celec
ubomíra Tthov 《European journal of oral sciences》2019,127(5):417-424
Despite the fact that saliva contains measurable concentrations of urea and creatinine, it is not widely used in clinical nephrology. One of the reasons is the high inter‐ and intra‐individual variability in the salivary markers of kidney function. We hypothesized that gingival bleeding in patients with periodontitis could contribute to this variability by increasing the concentration of salivary urea or creatinine. Samples were collected from 25 patients with periodontitis and 29 healthy controls. In addition, saliva samples from five healthy volunteers were artificially contaminated with blood. The concentration of urea, but not that of creatinine, was more than twice as high in patients with periodontitis than in controls. Artificial contamination of saliva with blood did not affect the salivary concentration of creatinine. Salivary urea increased only with very high levels of contamination (≥2.5% blood in saliva), but that did not occur in patients. In conclusion, periodontitis increases the concentration of salivary urea, but this is not likely to be a result of contamination with blood. Future studies should investigate the composition of the oral microbiome, specifically regarding how it affects the concentration of salivary urea. Salivary creatinine seems to be a more robust non‐invasive marker of renal functions than salivary urea. 相似文献
9.
Bacillus subtilis is considered to have great potential as a host for the production and secretion of recombinant proteins. Many different expression systems have been developed for B. subtilis. Here we compare two widely used expression systems, the IPTG-inducible derivative of spac system (hyper-spank) and the xylose-inducible (xyl) to the SURE (subtilin-regulated gene expression) system. Western blot analysis of the membrane protein SpoIISA together with its protein partner SpoIISB showed that the highest expression level of this complex is obtained using the SURE system. Measurement of β-galactosidase activities of the promoter-lacZ fusions in individual expression systems confirmed that the PspaS promoter of the SURE system is the strongest of those compared, although the induction/repression ratio reached only 1.84. Based on these results, we conclude that the SURE system is the most efficient of these three B. subtilis expression systems in terms of the amount of expressed product. Remarkably, the yield of the SpoIISA–SpoIISB complex obtained from B. subtilis was comparable to that normally obtained from the Escherichia coli arabinose-inducible expression system. 相似文献
10.
Kovalčíková Alexandra Gyurászová Marianna Vavrincová-Yaghi Diana Vavrinec Peter Tóthová Ľubomíra Boor Peter Šebeková Katarína Celec Peter 《Metabolic brain disease》2018,33(3):961-967
Metabolic Brain Disease - Uremic encephalopathy is a severe complication of renal failure. The underlying pathogenesis is unknown although several mechanisms have been suggested. Renal failure... 相似文献