pET28a-Streptag I-SbpA-Ag85B重组质粒的表达与鉴定

目的 应用分子克隆技术获得纳米级Ag85B蛋白并对其进行鉴定和免疫学特性检测.方法 利用S-layer/Streptag I这一具有自我组装能力的纳米模式嵌合结核杆菌分泌的Ag85B蛋白基因,将其克隆到原核表达载体pET28a（＋）中,并在大肠杆菌中表达Ag85B蛋白,表达后的蛋白经SDS-PAGE和Western blotting鉴定.结果 原核表达重组质粒pET28a-Streptag I-SbpA-Ag85B成功构建,并可在大肠杆菌中诱导表达,得到的Streptag I-Sbpa-Ag85B蛋白经SDS-PAGE和Western blotting鉴定正确,重组蛋白能被结核患者血清识别.结论 重组后的Ag85B蛋白具有较强的免疫反应性.所建立的原核表达体系为新型结核疫苗研制提供了一个新的思路.     

淮河水系毛毕吸虫的研究初报

目的调查淮河水系有无毛毕属吸虫存在及其尾蚴性皮炎而行。方法现场采集耳萝卜螺（Radixauricularia），用直接压片法分离尾蚴，并感染雏鸭；采集渔民养殖家鸭的粪便，分别用水洗沉淀法检查虫卵和孵化法分离毛蚴，并用此毛蚴感染实验室饲养的耳萝卜螺；解剖沿岸渔民放养的家鸭及人工实验感染的雏鸭分离成虫，并对该成虫作扫描电镜观察；对沿岸部分村民作现场查询和体检，以确定有无该皮炎表现及伴随症状。结果从现场捕获的耳萝卜螺体内可分离到有眼点的叉尾蚴，经鉴定为毛毕属吸虫尾蚴。从渔民养殖家鸭的粪便内可检获到虫卵，该卵可孵出毛蚴；毛蚴感染实验室内饲养的耳萝卜螺，可获与上述形态相同的有眼点的叉尾蚴。解剖上述两个不同来源的家鸭均可获毛毕属吸虫成虫。该毛毕吸虫经电镜观察发现其形态特征与毛毕属吸虫其它近似种有所不同，因此，暂名为淮南毛毕吸虫。结论淮河水系有毛毕属吸虫存在，其尾蚴可引起皮炎。     

毛毕吸虫成虫分离方法的研究初报

目的为提高毛毕吸虫成虫的收集率。方法对家鸭行离体或在体肝静脉逆行灌注收集肝内和门静脉内成虫;同时对家鸭灌注降主动脉,以收集肝内、门静脉内和肠系膜静脉的成虫。结果应用上述方法可收集到大量活的无损伤的成虫。结论灌注法优于解剖法,且简便、快捷,实用性强。     

Protective role of melatonin in progesterone production by human luteal cells

This study investigated whether melatonin protects luteinized granulosa cells from reactive oxygen species (ROS) as an antioxidant to enhance progesterone production in the follicle during ovulation. Follicular fluid was sampled at the time of oocyte retrieval in women undergoing in vitro fertilization and embryo transfer (IVF-ET). Melatonin concentrations in the follicular fluid were positively correlated with progesterone concentrations (r = 0.342, P < 0.05) and negatively correlated with the concentration of 8-hydroxy-2'-deoxyguanosine (8-OHdG), an oxidative stress marker (r = -0.342, P < 0.05). The progesterone and 8-OHdG concentrations were negatively correlated (r = -0.246, P < 0.05). Luteinized granulosa cells were obtained at the time of oocyte retrieval in women undergoing IVF-ET. Cells were incubated with H(2)O(2) (30, 50, 100 μm) in the presence or absence of melatonin (1, 10, 100 μg/mL). Progesterone production by luteinized granulosa cells was significantly inhibited by H(2)O(2). Melatonin treatment overcame the inhibitory effect of H(2) O(2) . Twenty-five patients who had luteal phase defect (serum progesterone concentrations <10 ng/mL during the mid-luteal phase) were divided into two groups during the next treatment cycle: 14 women were given melatonin (3 mg/day at 22:00 hr) throughout the luteal phase and 11 women were given no medication as a control. Melatonin treatment improved serum progesterone concentrations (>10 ng/mL during the mid-luteal phase) in nine of 14 women (64.3%), whereas only two of 11 women (18.1%) showed normal serum progesterone levels in the control group. In conclusion, melatonin protects granulosa cells undergoing luteinization from ROS in the follicle and contributes to luteinization for progesterone production during ovulation.     

微创清除术治疗脑出血的临床研究

 目的 为研究治疗高血压脑出血的有效方法,提高治愈率,减少致残率。方法根据脑出血患者CT结果,选择出血量在30～80ml,意识Ⅱ～Ⅳ级的住院患者50例,施行微创清除术。选择1997年～2000年相同条件的内科治疗和传统外科治疗各50例作为对照。疗效判定采用ADL分级法。结果 微创治疗组在血肿清除时间、死亡率、恢复时间等方而均明显优于其他两组。结论微创清除术治疗脑出血明显提高高血压脑出血患者的生存率,降低致残率,缩短住院时间,值得推广应用。     

复方斑蝥胶囊联合化疗治疗弥漫大B细胞淋巴瘤的临床研究

目的 探讨复方斑蝥胶囊联合环磷酰胺+表柔比星+长春新碱+泼尼松(CHOP方案)治疗弥漫大B细胞淋巴瘤(DLBCL)的临床疗效.方法 回顾性分析73例DLBCL患者的临床资料,根据治疗方法的不同将患者分为复方斑蝥胶囊联合CHOP组(n=37)和CHOP组(n=36),比较两组患者的临床有效率、生存率、无进展生存率和不良反应发生率.结果 治疗后复方斑蝥胶囊联合CHOP组中,完全缓解(CR)21例,部分缓解(PR)10例,总有效率为83.8％(31/37);CHOP组中,CR 15例,PR 7例,总有效率为61.1％(22/36),两组患者的总有效率比较,差异有统计学意义(P﹤0.05).对于国际预后指数(IPI)评分为3～5分、Ki-67≥80％及β2微球蛋白升高的患者,复方斑蝥胶囊联合CHOP组的有效率分别为85.7％、90.5％和90.0％,均高于CHOP组的55.0％、61.9％和57.1％,差异均有统计学意义(P﹤0.05).两组患者的1年总生存率、1年无进展生存率及不良反应发生率比较,差异均无统计学意义(P﹥0.05).结论 复方斑蝥胶囊联合CHOP方案治疗DLBCL的临床有效率高于CHOP方案,且不良反应无明显增多,值得临床推广应用.     

Brain-derived neurotrophical factor after olfactory ensheathing cells transplantation in spinal cord injury of rats

<正>Objective To observe the expression of brain - derived neurotrophical factor ( BDNF) in injury spinal cord after transplantation olfactory ensheathing cells ( OECs) , and to investigate the mechanism of OECs repairing spinal cord injury. Methods OECs from GFP transgenic rats were separated and cultured for transplantation. Spinal cord injury rats were separated two groups by random     

MiR-1193 was sponged by LINC00963 and inhibited cutaneous squamous cell carcinoma progression by targeting SOX4

Cutaneous squamous cell carcinoma (CSCC), a class of skin tumor derived from epidermal keratinocyte, is reputed as one of the most malignant tumors globally. MicroRNAs (miRNAs) are increasingly identified as essential players in CSCC. Current study aimed to uncover the impact and mechanism of miR-1193 in CSCC. We identified the low expression of miR-1193 in CSCC cell lines. Gain- and loss-of-function assays showed that miR-1193 acted as an inhibitor of proliferation and migration in CSCC cells. Furthermore, we illustrated that miR-1193 targeted and inhibited SRY-box 4 (SOX4), and that long intergenic non-protein coding RNA 963 (LINC00963) sponged miR-1193 to upregulate SOX4 expression. Rescue assays showed that LINC00963 regulated CSCC progression through miR-1193/SOX4 axis. In conclusion, our study firstly revealed the LINC00963/miR-1193/SOX4 axis in CSCC, indicating miR-1193 as a promising biological target in CSCC progression.     

Relationship between post-stroke depression and lesion location: a meta-analysis

Our understanding of the relationship between the neuroanatomic loci of brain damage and the incidence of post-stroke depression (PSD) is not complete. Many studies have investigated this relationship and the evidence is conflicting. With the purpose of gaining a consistent, strong, and credible conclusion on the relationship between PSD and the loci of brain damage, a meta-analysis was used in this study to systematically reanalyze the findings of related studies and to investigate the sources of heterogeneity among study results. The key words "stroke or cerebrovascular" and "depression or mood or affective" were entered into the MEDLINE, PsycINFO, and EMBASE databases to search for relevant studies. The references cited in the studies found were also used to locate additional studies. For each eligible study, the important study characteristics were recorded, and the effect sizes of the relationship between PSD and lesion location were computed. Furthermore, we conducted subgroup analyses to explore the heterogeneity among study results. A total of 3,668 patients participating in 52 studies were included in this meta-analysis. There was a weak relationship between PSD and right hemisphere lesion. The major sources of heterogeneous study results included systematic exclusion of patients with language dysfunction and use of different assessors and instruments for diagnosing depression. Future efforts should aim to enhance standards for reporting studies, improve assessment tools for assessing depression of aphasic patients, and adopt appropriate study methodologies for investigating the relationship between PSD and lesion location.