全文获取类型
收费全文 | 281篇 |
免费 | 19篇 |
国内免费 | 2篇 |
专业分类
耳鼻咽喉 | 13篇 |
儿科学 | 3篇 |
妇产科学 | 11篇 |
基础医学 | 33篇 |
口腔科学 | 8篇 |
临床医学 | 18篇 |
内科学 | 65篇 |
皮肤病学 | 11篇 |
神经病学 | 8篇 |
特种医学 | 2篇 |
外科学 | 34篇 |
综合类 | 4篇 |
预防医学 | 17篇 |
眼科学 | 23篇 |
药学 | 20篇 |
中国医学 | 3篇 |
肿瘤学 | 29篇 |
出版年
2024年 | 1篇 |
2023年 | 1篇 |
2022年 | 5篇 |
2021年 | 10篇 |
2020年 | 6篇 |
2019年 | 10篇 |
2018年 | 11篇 |
2017年 | 13篇 |
2016年 | 13篇 |
2015年 | 9篇 |
2014年 | 14篇 |
2013年 | 20篇 |
2012年 | 25篇 |
2011年 | 21篇 |
2010年 | 10篇 |
2009年 | 12篇 |
2008年 | 17篇 |
2007年 | 17篇 |
2006年 | 8篇 |
2005年 | 9篇 |
2004年 | 13篇 |
2003年 | 6篇 |
2002年 | 7篇 |
2001年 | 2篇 |
2000年 | 1篇 |
1999年 | 6篇 |
1998年 | 2篇 |
1997年 | 4篇 |
1996年 | 1篇 |
1995年 | 1篇 |
1993年 | 1篇 |
1992年 | 1篇 |
1991年 | 2篇 |
1990年 | 3篇 |
1989年 | 4篇 |
1987年 | 2篇 |
1986年 | 2篇 |
1985年 | 4篇 |
1984年 | 4篇 |
1983年 | 3篇 |
1980年 | 1篇 |
排序方式: 共有302条查询结果,搜索用时 15 毫秒
1.
Olle Ringdén Marie Schaffer Katarina Le Blanc Ulla Persson Dan Hauzenberger Mohammad R Abedi Olle Olerup Per Ljungman Mats Remberger 《Biology of blood and marrow transplantation》2004,10(2):128-134
The aim of this study was to identify significant prognostic factors by using unrelated genomically HLA-A, -B and -DRB1-identical donors. Such data could help to choose the best donor. We studied 136 consecutive patients with hematologic malignancies and a median age of 32 years (range, 0-55 years) who received hematopoietic stem cell transplantation. Bone marrow grafts were given to 83 and peripheral blood stem cells to 53 patients. The cumulative incidence of grade II to IV acute graft-versus-host disease (GVHD) was 30% and of chronic GVHD was 54%. At 5 years, the overall transplant-related mortality (TRM) was 34%, and patient survival was 50%. In Cox multivariate analysis, 32 potential risk factors were analyzed. Monoclonal antibody OKT-3 during conditioning was correlated with grade II to IV acute GVHD, chronic GVHD, and TRM. HLA-DP mismatch was associated with poor TRM and poor survival. Cytomegalovirus-seropositive patients with a seronegative donor had a decreased leukemia-free survival. Five-year TRM was 14% with no risk factor, 38% with 1 risk factor, and 87% with 2 risk factors. The 5-year survival was 72%, 48%, and 30% with 0, 1, and 2 risk factors, respectively. We concluded that unrelated hematopoietic stem cell transplantation may be improved if an optimal donor and immunosuppression are chosen. 相似文献
2.
3.
Equine piroplasmosis is a tickborne disease of equids with worldwide distribution, caused by Theileria equi and Babesia caballi. The aim of this study was molecular detection of T. equi and B. caballi in donkeys in northeastern Iran and investigate the association between positivity of piroplasm infection and host-related factors. In the present study, Blood samples were collected from 106 apparently healthy donkeys (Equus asinus) in North Khorasan province, Iran. Blood smears were prepared and stained by giemsa method. DNA was extracted from blood and then multiplex-PCR was done for detection of any piroplasms infection. According to the results, four donkeys showed T. equi in blood smears but B. caballi was not found. Also, fifty four donkeys (50.94%) showed T. equi infection using multiplex-PCR. No siginificant difference was observed between the frequency of T. equi infection with host-related factors in donkeys. This is the first report on the molecular detection of eqiune piroplamosis in donkeys in Iran. Also, no significant association was found between the rate of T. equi infected animals.Key Words: Babesia caballi, Donkey, PCR, Theileria equi 相似文献
4.
Arezou Masteri Farahani Fariba Maleki Nourollah Sadeghzadeh Saeid Abediankenari Seyed Mohammad Abedi Mostafa Erfani 《Chemical biology & drug design》2020,96(2):850-860
Prostate cancer is a serious threat to men's health, so it is necessary to develop the techniques for early detection of this malignancy. Radiolabeled peptides are the useful tools for diagnosis of prostate cancer. In this research, we designed a new HYNIC‐conjugated GnRH analogue and labeled it by 99mTc with tricine/EDDA as coligands. We used aminohexanoic acid (Ahx) as a hydrocarbon linker to generate 99mTc‐(tricine/EDDA)‐HYNIC‐Ahx‐[DLys6]GnRH. The radiopeptide exhibited high radiochemical purity and stability in solution and serum. Two human prostate cancer cell lines LN‐CaP and DU‐145 were used for cellular experiments. The binding specificity and affinity of radiopeptide for LN‐CaP were superior to DU‐145 cells. The Kd values for LN‐CaP and DU‐145 cells were 41.91 ± 7.03 nM and 55.96 ± 10.56 nM, respectively. High kidney uptake proved that the main excretion route of radiopeptide was through the urinary system. The tumor/muscle ratio of 99mTc‐HYNIC‐Ahx‐[DLys6]GnRH was 4.14 at 1 hr p.i. that decreased to 2.41 at 4 hr p.i. in LN‐CaP tumor‐xenografted nude mice. The blocking experiment revealed that the tumor uptake was receptor‐mediated. The lesion was visualized clearly using 99mTc‐[DLys6]GnRH at 1 hr p.i. Accordingly, this research highlights the capability of 99mTc‐(tricine/EDDA)‐HYNIC‐Ahx‐[DLys6]GnRH peptide as a promising agent for GnRHR‐expressing tumor imaging. 相似文献
5.
6.
7.
Jairajpuri DS Fatima S Saleemuddin M 《Clinica chimica acta; international journal of clinical chemistry》2007,378(1-2):86-92
BACKGROUND: Immunoglobulins undergo non-enzymatic glycation reaction with sugars both in vivo and in vitro. Effects of glycation on the ability of the antibodies to bind antigens are contradictory. Antibodies raised in various animals may also be exposed to high concentration of sugars that are added during freeze-drying/pasteurization for preservation. METHODS: IgG isolated from the sera of goat, human, rabbit, mouse, buffalo as well as IgY from hen egg yolk was subjected to in vitro glycation with fructose. The behavior of glycated IgG was investigated by SDS-PAGE, hyperchromicity at 280 nm, tryptophan fluorescence and new fluorescence. RESULTS: Marked variations were observed in the response of the immunoglobulins derived from various animals to incubation with fructose. Also, incubation of anti-glucoseoxidase (GOD) antibodies with fructose resulted in a rapid loss of their ability to bind the enzyme antigen as revealed by immunodiffusion and ELISA. DETAPAC and EDTA were quite protective but were unable to completely prevent the fructose-induced alterations. CONCLUSIONS: Immunoglobulins derived from goat, human, rabbit, mouse, buffalo and hen egg yolk undergo remarkable structural alterations on incubation with fructose. The susceptibility of the immunoglobulins to the modification however differed remarkably. The goat IgG was most recalcitrant while hen egg yolk IgY was most susceptible to the alterations. DETAPAC or EDTA restricted the fructose-induced alterations remarkably. 相似文献
8.
Jay L. Patel Mehrdad Abedi Christopher R. Cogle Harry P. Erba Kathryn Foucar Guillermo Garcia-Manero David L. Grinblatt Rami S. Komrokji Sandra E. Kurtin Jaroslaw P. Maciejewski Daniel A. Pollyea Dennis A. Revicki Gail J. Roboz Michael R. Savona Bart L. Scott Mikkael A. Sekeres David P. Steensma Michael A. Thompson Elizabeth Dawn Flick Pavel Kiselev Chrystal U. Louis Melissa Nifenecker Arlene S. Swern Tracy I. George 《International journal of laboratory hematology》2021,43(3):426-432
9.
Christopher S. Mirucki Mehran Abedi Jin Jiang Qiang Zhu Yu-Hsiung Wang Kamran E. Safavi Robert B. Clark Frank C. Nichols 《Journal of endodontics》2014
Introduction
Periapical infections secondary to pulpal necrosis are associated with bacterial contamination of the pulp. Porphyromonas endodontalis, a gram-negative organism, is considered to be a pulpal pathogen. P. gingivalis is phylogenetically related to P. endodontalis and synthesizes several classes of novel complex lipids that possess biological activity, including the capacity to promote osteoclastogenesis and osteoclast activation. The purpose of this study was to extract and characterize constituent lipids of P. endodontalis and evaluate their capacity to promote proinflammatory secretory responses in the macrophage cell line, RAW 264.7, as well as their capacity to promote osteoclastogenesis and inhibit osteoblast activity.Methods
Constituent lipids of both organisms were fractionated by high-performance liquid chromatography and were structurally characterized using electrospray mass spectrometry or electrospray-mass spectrometry/mass spectrometry. The virulence potential of P. endodontalis lipids was then compared with known biologically active lipids isolated from P. gingivalis.Results
P. endodontalis total lipids were shown to promote tumor necrosis factor alpha secretion from RAW 264.7 cells, and the serine lipid fraction appeared to account for the majority of this effect. P. endodontalis lipid preparations also increased osteoclast formation from RAW 264.7 cells, but osteoblast differentiation in culture was inhibited and appeared to be dependent on Toll-like receptor 2 expression.Conclusions
These effects underscore the importance of P. endodontalis lipids in promoting inflammatory and bone cell activation processes that could lead to periapical pathology. 相似文献10.
Farshad Abedi Bibi Marjan Razavi Hossein Hosseinzadeh 《Phytotherapy research : PTR》2020,34(4):729-741
Beneficial therapeutic effects of phenolic acids have been proven in various research projects including in vivo and in vitro studies. Gentisic acid (GA) is a phenolic acid that has been associated with useful effects on human health, such as antiinflammatory, antigenotoxic, hepatoprotective, neuroprotective, antimicrobial, and especially antioxidant activities. It is an important metabolite of aspirin and also widely distributed in plants as a secondary plant product such as Gentiana spp., Citrus spp., Vitis vinifera, Pterocarpus santalinus, Helianthus tuberosus, Hibiscus rosa‐sinensis, Olea europaea, and Sesamum indicum and in fruits such as avocados, batoko plum, kiwi fruits, apple, bitter melon, black berries, pears, and some mushrooms. This study was undertaken to review the pharmacological effects, pharmacokinetic properties as well as toxicity and pharmaceutical applications of GA. 相似文献