Graves病硫脲类药物治疗所致甲减（附32例临床分析）

Ｇｒａｖｅｓ病硫脲类药物治疗所致甲减（附３２例临床分析）广东省广宁县人民医院内科（５２６３００）梁如庆广州军区广州总医院内分泌科王国华，林延德Ｇｒａｖｅｓ病（弥漫性甲状腺肿伴甲状腺功能亢进症）的治疗方面，鉴于多数患者对手术有顾虑，同位素碘治疗尚不能普...     

脂氧素A4对心肌缺血再灌注损伤的保护作用

Objective To investigate the roles of lipoxin A4, an endegenous lipid mediator with wide anti-inflammatory fea- tures, in attenuating myocardial ischemia-reperfusion injury and the possible mechanisms. Methods Thirty male KM mice were divid- ed randomly into three groups, 10 in each: ischemia-reperfusion group (group A), lipoxin A4 (0.1 mg/kg) group (group B) and Zn- PP (Zinc protoporphyrin Ⅸ, 25 mg/kg)phus lipoxin A4 (0.1 mg/kg)group (group C). A ischemia-reperfusion heart model was de- veloped by ligating the lift anterior descending branch of the coronary artery. A dine of 10% dehydrated alcohol in 0.2 ml for group A, a dose of isochoric lipoxin A4 for group B and a dose of isochoric ZnPP + lipoxin A4 for group C was infused into the ascending aorta through a catheter, which was kpassing the right common carotid artery, 30 minutes after reperfusion. The concentration of serum TNF-α, activities of serum crestine kinase(CK) and lactate dehydrogenase (LDH), activities of myeloperoxidase (MPO) and malond- ialdehyde (MDA) and the cell apoptosis rate in the myocardial tissue were measured 5 hours after reperfusion. Pathological features of the inflammatory infiltration in the myocardium were also observated.Results As compared with group A, the inflammatoryry infiltra- tion in the ischemic and necrotic regions tithe myocardium was reduced, with group C in the intermediate range. The serum activities of CK and LDH wine significantly lower in group B and C than that in group A, and the lowest activities were detected in group B. Similar findings were observed for MPO, an indicator for neutrophil infiltration, and MDA, an indictor for cell injury caused by oxy- gen radicals, in the myocardium. The concentration of TNF-α and the rate of cadiocyte apoptosis were decreased significantly in group B(P < 0.01). ZnPP, an inhibitor of heine oxygenase (HO)-1, attenusted the above protective effects of lipoxin A4 significantly (P<0.05). Conclusion Lipoxin A4 has protective effects against myocardial ischemia-reperfusion injury, and HO-1 may have a potential role in the protectve mechanisms of lipoxin A4, probably pertly by means of reducing the production of reactive oxygen spe- cies and TNF-α, decreasing the activation and infiltration of neutrophils, alleviating inflammatory damage and avoiding apoptosis.     

老年人高血压性脑出血急性期T淋巴细胞亚群的检测及初步探讨

老年人高血压性脑出血急性期Ｔ淋巴细胞亚群的检测及初步探讨王国华，张秀华，白颖哈市第一医院神经科王文宝哈市第一医院血液病研究所祁威，方俊兰哈市第十医院神经科自从１９７９年Ｒｅｉｎｈｅｒｚ获得淋巴细胞单克隆抗体以来，使人们对Ｔ淋巴细胞亚群及其临床意义逐渐...     

清胃散加味治疗腮腺炎的体会

流行性腮腺炎是由腮腺炎病毒所引起的急性呼吸道传染病 ,好发于儿童时期 ,亦可见于成人。1　资料与方法1.1　一般资料 :从 1999年 5月至 2 0 0 2年 7月共治疗病例12例 ,其中男性 9例 ,女性 3例 ,年龄 5～ 41岁。1.2　治疗方法 :1处方 :当归 15 g,生地黄 2 0 g,黄连 10 g,升麻 15 g,丹皮 12 g,石膏 (先煎 ) 3 0 g,麻黄 9g,板蓝根 10g。 2用法 :先煎石膏 3 0 min,再入他药 ,煎药用水超过药面2 cm高 ,煎煮 2次后 ,将药液混合均匀 ,分早晚服。服药 2～5剂。2　结　　果痊愈 8例 ,有效 3例 ,无效 1例 ,总有效率为 91.7%。3　讨　　论流行性腮腺炎…     

新生儿窒息心电图改变及其意义

本文对我院1992年6月～1996年10月72例新生儿窒息患儿心电图进行观察分析，以此指导临床用药。临床资料本组男37例，女35例；早产儿16例，足月小样儿9例，足月新生儿47例。轻度窒息49例，重度23例。观察时间：出生2d内有心电图异常者，治疗1wk后复查。新生儿窒息患儿心电图表现     

贫困大学生心理健康分析与对策

随着高校收费制度的改革 ,“贫困生”在大学中占一定比例 ,其心理问题也较复杂。因而 ,研究贫困大学生心理特点 ,帮助他们培养健康的心理素质 ,使他们不仅具有较高的专业知识水平 ,而且具有完善的人格 ,顺利渡过大学时代 ,对于高校学生思想政治工作者来说是一项紧迫的任务。哈尔滨医科大学仅 2 0 0 0年入学的一个系70 0多名新生中 ,就有 93名贫困生 ,占新生总数的 13 %。调查表明 ,贫困生多数来自于农村及边远地区 ,其中一部分来自灾区 ,家中兄弟姐妹较多 ,或父母及亲属患有重病 ,造成家中负债 ,另外 ,有一些贫困生家住城市 ,但父母下岗 ,或…     

Weel基因修饰细胞抗CTL诱导的凋亡效应

目的 研究Weel转基因细胞抗CTL介导的凋亡效应及抗穿孔素抗体的对此抗凋亡效应的影响。方法 采用DNA转染技术将重组体Weel Hu／GFP导入哺乳动物细胞NIT(鼠胰岛细胞瘤细胞)；以转染重组体前、后的NIT为靶细胞，采用ELISA凋亡试剂盒检测CTL介导的凋亡效应。结果 转染Weel及加入抗穿孔素抗体的NIT组发生凋亡的细胞数最少。结论 Weel基因转染靶细胞在一定程度上可抵抗CTL介导的细胞凋亡，而穿孔素抗体可协同增强此抗凋亡效应。     

应用免疫金标记技术分析福氏2a痢疾杆菌膜抗原

本文报告应用抗福氏２ａ痢杆菌膜成份ＭｃＡｂ及胶体金探针对细菌膜抗原进行了定位。结果显示４种抗脂多糖ＭｃＡｂ（３Ａ６，２Ｅ６，４Ｅ６，４Ｆ１，２Ａ３）可不均一的识别位于细菌表面的抗原，而另外一种抗ＬＰＳＭｃＡｂ（１Ｇ８）和一抗膜蛋白ＭｃＡｂ（１Ａ１）识别的抗朱未暴露于细菌表面。应用细菌膜碎片包埋前染色成功地定位了抗膜蛋白ＭｃＡｂ１Ａ１位于细菌内膜的抗原。与ＭｃＡｂ的生物学活性比较表明。阳性标记细菌表