奇静脉逆行灌注在主动脉手术中对脊髓保护的解剖学研究

目的 :为主动脉手术中脊髓保护提供形态基础和术式设计。方法 :解剖观察了 3 1例成人标本的奇静脉系、椎静脉丛 ,以及脊髓静脉回流途径。结果 :观测奇静脉系及相关静脉 ,其直径为 ( x±s) :奇静脉为 (9.2± 1.9)mm ,半奇静脉为 (5 .5± 1.2 )mm ,副半奇静脉为 (3 .8± 0 .9)mm ,左最上肋间静脉为 (2 .0±0 .4)mm ,右最上肋间静脉为 (1.9± 0 .4)mm ,左腰升静脉为 (2 .3± 0 .5 )mm ,右腰升静脉为 (2 .2± 0 .6)mm。根据奇静脉属支的配布和肋间静脉汇入形式 ,将奇静脉系分为 4种类型。奇静脉及其属支中存在静脉瓣 ,有个体差异。脊髓静脉密集 ,与椎内静脉丛有丰富的网络连接 ,并通过神经根静脉与椎外静脉丛、奇静脉系相交通。结论 :在主动脉手术中应用奇静脉逆行灌注脊髓保护 ,是一种行之有效的新术式。     

坐骨棘筋膜固定术的临床解剖学研究

Objective The study was to explore the safety, firmness and convenience of the fascia around the ischial spine as a new fixation site for the vaginal fornix. Methods Between June 2007 and January 2008, detailed dissections and related measurements of the regions around the ischial spine were performed on 10 Chinese female cadavers (3 unembalmed and 7 embalmed cadavers). At the same time, the sacrospinous ligament,the fascia on the ischial spine, the iliococcygeus fascia as well as the vaginal fornix were exposed and the pull-out strength sequentially tested using a digital push-pull force gauge. Results The fascia on the ischial spine was firm and strong, with a thickness of 3 about mm. No major vessels or nerves were observed on the ischial spine. The greatest pullout strengths of the sacrospinous ligament,the fascia on the ischial spine, the iliococcygeus fascia as well as the vaginal fornix were (102±26),(64±15),(33±8)and(32±6)N, respectively. Conclusion The fascia at 1 cm from anterior lateral ischial spine, free of major vessels and nerves, is safe and strong and could be used as a new site for suspension in vaginal prolapse.     

坐骨棘筋膜固定术的临床解剖学研究

Objective The study was to explore the safety, firmness and convenience of the fascia around the ischial spine as a new fixation site for the vaginal fornix. Methods Between June 2007 and January 2008, detailed dissections and related measurements of the regions around the ischial spine were performed on 10 Chinese female cadavers (3 unembalmed and 7 embalmed cadavers). At the same time, the sacrospinous ligament,the fascia on the ischial spine, the iliococcygeus fascia as well as the vaginal fornix were exposed and the pull-out strength sequentially tested using a digital push-pull force gauge. Results The fascia on the ischial spine was firm and strong, with a thickness of 3 about mm. No major vessels or nerves were observed on the ischial spine. The greatest pullout strengths of the sacrospinous ligament,the fascia on the ischial spine, the iliococcygeus fascia as well as the vaginal fornix were (102±26),(64±15),(33±8)and(32±6)N, respectively. Conclusion The fascia at 1 cm from anterior lateral ischial spine, free of major vessels and nerves, is safe and strong and could be used as a new site for suspension in vaginal prolapse.     

超声波作用下NaBH4/ZrCl4还原9(E)-红霉素肟制备9(S)-红霉胺

9(S)-红霉胺是合成地红霉素和CP-544372的中间体，此化合物可在超声波的作用下利用NaBH4／ZrCl4于室温下还原9(E)-红霉素肟制得，收率为69％，其结构经IR、NMR和MS确证。该方法是制备9(S)-红霉胺的方便、有效的途径。     

4种9,10-二氢菲类化合物的体外抗肿瘤活性及机制

 目的研究4种9,10-二氢菲类化合物对4种人癌细胞的增殖抑制作用及其作用机制。方法采用MTT法测定它们对人肝癌细胞株HepG2,人非小细胞肺癌细胞株NCI-H460,人乳腺癌细胞株MCF-7,人神经胶质瘤细胞株SF-268的增殖抑制作用;采用流式细胞仪技术和蛋白质电泳技术检测它们对HepG2细胞的细胞周期以及细胞周期蛋白表达的影响。结果9,10-二氢菲类化合物对HepG2细胞的增殖有一定的抑制作用;其中4,5-二羟基-2-甲氧基-9,10-二氢菲可能通过下调周期蛋白CyclinB1的表达将HepG2细胞阻滞在G2/M期。结论9,10-二氢菲类化合物通过将HepG2细胞阻滞在G2/M期从而对其增殖产生了一定的抑制作用。     

坐骨棘筋膜固定术的临床解剖学研究

Objective The study was to explore the safety, firmness and convenience of the fascia around the ischial spine as a new fixation site for the vaginal fornix. Methods Between June 2007 and January 2008, detailed dissections and related measurements of the regions around the ischial spine were performed on 10 Chinese female cadavers (3 unembalmed and 7 embalmed cadavers). At the same time, the sacrospinous ligament,the fascia on the ischial spine, the iliococcygeus fascia as well as the vaginal fornix were exposed and the pull-out strength sequentially tested using a digital push-pull force gauge. Results The fascia on the ischial spine was firm and strong, with a thickness of 3 about mm. No major vessels or nerves were observed on the ischial spine. The greatest pullout strengths of the sacrospinous ligament,the fascia on the ischial spine, the iliococcygeus fascia as well as the vaginal fornix were (102±26),(64±15),(33±8)and(32±6)N, respectively. Conclusion The fascia at 1 cm from anterior lateral ischial spine, free of major vessels and nerves, is safe and strong and could be used as a new site for suspension in vaginal prolapse.     

大黄中蒽醌类成分分离方法的改进

<正> 大黄是一味重要的常用中药,具有泻下,清热、解毒等作用。大黄含有多种蒽醌类化合物,其中大黄酸(Rhein)具有羧基,酸性最强。大黄素(Emodin)具有β-酚羟基,酸性第二。芦荟大黄素(Aloeemodin)联有羟甲基,酸性第三。大黄素甲醚(physcion)及大黄酚(chrysophanol)     

红葱萃取物对离体大鼠主动脉血管环的影响研究

目的:研究红葱萃取物(氯仿和乙酸乙酯萃取物)对离体大鼠胸主动脉血管环的影响作用。方法:将大鼠分为3组,即空白和氯仿、乙酸乙酯萃取物组,用Powerlab生物信号采集与分析系统测定离体主动脉血管环张力的变化。结果:与空白组比较,氯仿和乙酸乙酯萃取物组的血管环明显舒张,对苯肾上腺素(PE)和KCl引发的预收缩有显著抑制作用(P<0.05或P<0.01)。氯仿部位较乙酸乙酯部位萃取效果好(P<0.05或P<0.01)。结论:红葱的氯仿和乙酸乙酯萃取部位既能直接舒张血管环,亦能抑制PE和KCl对血管环的预收缩作用。     

红葱抗稻瘟霉活性成分研究

目的阐明红葱抗稻瘟霉活性的物质基础.方法利用稻瘟霉筛选体系对红葱的60%(φ)乙醇提取物及其氯仿、正丁醇和水的萃取部分进行活性筛选,并对活性部位的化学成分进行分离鉴定.结果与结论从活性部位中分离得到10个化合物,通过理化性质和波谱数据鉴定它们的结构分别为:异红葱甲素(1)、异红葱乙素(2)、红葱乙素(3)、β-谷甾醇(4)、8-羟基-3,4-二甲氧基-1-甲基-蒽醌-2-羧酸甲酯(5)、4-羟基红葱乙素(6)、hongconin(7)、4,8-二羟基-3-甲氧基-1-甲基-蒽醌-2-羧酸甲酯(8)、dihydro-eleutherinol(9)、1,3,6-三羟基-8-甲基蒽醌(10).其中,9和10为首次从该属植物中分得.化合物2、3、5、6、7、9、10具有较好的诱导稻瘟霉菌丝变形的活性,它们的最小变形浓度(minimummorphologicaldeformationconcentrate,MMDC)分别为30、30、170、130、55、60、150μmol·L-1.用MTT法测定10个化合物的抗肿瘤活性,发现化合物2、3、5、6、7、9有较强的抑制人类红白血病细胞株K562细胞生长的活性,它们的IC50值分别为33、49、266、35、174、154μmol·L-1.     

腹壁下动脉穿支皮瓣的局部解剖

目的探讨腹壁下动脉穿支皮瓣的解剖学基础及其在头颈修复中的临床意义。方法解剖国人新鲜成人尸体5具,观察并测量腹壁下深动脉穿支（deep inferior epigastric artery perforator,DIEAP）皮瓣供血穿支的数目、类型、管径、蒂长、走行、出筋膜后轴向及源血管外径并对其定位。结果腹壁下动脉穿支数目（〉0.5mm）为124支,平均每侧（12.4±3.01）支,均为肌皮支。优势穿支的平均蒂长（14.5±3.13）cm,位置位于脐外6cm,脐上、下3cm之内。腹壁下动、静脉外径为（2.77±0.31）mm～（3.0±0.26）mm、（2.58±0.4）mm～（3.1±0.42）mm。结论DIEAP皮瓣穿支血管解剖位置较为恒定,其管径及蒂长均适宜头颈肿瘤术后缺损的修复。