奇静脉逆行灌注在主动脉手术中对脊髓保护的解剖学研究

目的 :为主动脉手术中脊髓保护提供形态基础和术式设计。方法 :解剖观察了 3 1例成人标本的奇静脉系、椎静脉丛 ,以及脊髓静脉回流途径。结果 :观测奇静脉系及相关静脉 ,其直径为 ( x±s) :奇静脉为 (9.2± 1.9)mm ,半奇静脉为 (5 .5± 1.2 )mm ,副半奇静脉为 (3 .8± 0 .9)mm ,左最上肋间静脉为 (2 .0±0 .4)mm ,右最上肋间静脉为 (1.9± 0 .4)mm ,左腰升静脉为 (2 .3± 0 .5 )mm ,右腰升静脉为 (2 .2± 0 .6)mm。根据奇静脉属支的配布和肋间静脉汇入形式 ,将奇静脉系分为 4种类型。奇静脉及其属支中存在静脉瓣 ,有个体差异。脊髓静脉密集 ,与椎内静脉丛有丰富的网络连接 ,并通过神经根静脉与椎外静脉丛、奇静脉系相交通。结论 :在主动脉手术中应用奇静脉逆行灌注脊髓保护 ,是一种行之有效的新术式。     

坐骨棘筋膜固定术的临床解剖学研究

Objective The study was to explore the safety, firmness and convenience of the fascia around the ischial spine as a new fixation site for the vaginal fornix. Methods Between June 2007 and January 2008, detailed dissections and related measurements of the regions around the ischial spine were performed on 10 Chinese female cadavers (3 unembalmed and 7 embalmed cadavers). At the same time, the sacrospinous ligament,the fascia on the ischial spine, the iliococcygeus fascia as well as the vaginal fornix were exposed and the pull-out strength sequentially tested using a digital push-pull force gauge. Results The fascia on the ischial spine was firm and strong, with a thickness of 3 about mm. No major vessels or nerves were observed on the ischial spine. The greatest pullout strengths of the sacrospinous ligament,the fascia on the ischial spine, the iliococcygeus fascia as well as the vaginal fornix were (102±26),(64±15),(33±8)and(32±6)N, respectively. Conclusion The fascia at 1 cm from anterior lateral ischial spine, free of major vessels and nerves, is safe and strong and could be used as a new site for suspension in vaginal prolapse.     

坐骨棘筋膜固定术的临床解剖学研究

Objective The study was to explore the safety, firmness and convenience of the fascia around the ischial spine as a new fixation site for the vaginal fornix. Methods Between June 2007 and January 2008, detailed dissections and related measurements of the regions around the ischial spine were performed on 10 Chinese female cadavers (3 unembalmed and 7 embalmed cadavers). At the same time, the sacrospinous ligament,the fascia on the ischial spine, the iliococcygeus fascia as well as the vaginal fornix were exposed and the pull-out strength sequentially tested using a digital push-pull force gauge. Results The fascia on the ischial spine was firm and strong, with a thickness of 3 about mm. No major vessels or nerves were observed on the ischial spine. The greatest pullout strengths of the sacrospinous ligament,the fascia on the ischial spine, the iliococcygeus fascia as well as the vaginal fornix were (102±26),(64±15),(33±8)and(32±6)N, respectively. Conclusion The fascia at 1 cm from anterior lateral ischial spine, free of major vessels and nerves, is safe and strong and could be used as a new site for suspension in vaginal prolapse.     

超声波作用下NaBH4/ZrCl4还原9(E)-红霉素肟制备9(S)-红霉胺

9(S)-红霉胺是合成地红霉素和CP-544372的中间体，此化合物可在超声波的作用下利用NaBH4／ZrCl4于室温下还原9(E)-红霉素肟制得，收率为69％，其结构经IR、NMR和MS确证。该方法是制备9(S)-红霉胺的方便、有效的途径。     

坐骨棘筋膜固定术的临床解剖学研究

Objective The study was to explore the safety, firmness and convenience of the fascia around the ischial spine as a new fixation site for the vaginal fornix. Methods Between June 2007 and January 2008, detailed dissections and related measurements of the regions around the ischial spine were performed on 10 Chinese female cadavers (3 unembalmed and 7 embalmed cadavers). At the same time, the sacrospinous ligament,the fascia on the ischial spine, the iliococcygeus fascia as well as the vaginal fornix were exposed and the pull-out strength sequentially tested using a digital push-pull force gauge. Results The fascia on the ischial spine was firm and strong, with a thickness of 3 about mm. No major vessels or nerves were observed on the ischial spine. The greatest pullout strengths of the sacrospinous ligament,the fascia on the ischial spine, the iliococcygeus fascia as well as the vaginal fornix were (102±26),(64±15),(33±8)and(32±6)N, respectively. Conclusion The fascia at 1 cm from anterior lateral ischial spine, free of major vessels and nerves, is safe and strong and could be used as a new site for suspension in vaginal prolapse.     

红葱萃取物对离体大鼠主动脉血管环的影响研究

目的:研究红葱萃取物(氯仿和乙酸乙酯萃取物)对离体大鼠胸主动脉血管环的影响作用。方法:将大鼠分为3组,即空白和氯仿、乙酸乙酯萃取物组,用Powerlab生物信号采集与分析系统测定离体主动脉血管环张力的变化。结果:与空白组比较,氯仿和乙酸乙酯萃取物组的血管环明显舒张,对苯肾上腺素(PE)和KCl引发的预收缩有显著抑制作用(P<0.05或P<0.01)。氯仿部位较乙酸乙酯部位萃取效果好(P<0.05或P<0.01)。结论:红葱的氯仿和乙酸乙酯萃取部位既能直接舒张血管环,亦能抑制PE和KCl对血管环的预收缩作用。     

红葱抗稻瘟霉活性成分研究

目的阐明红葱抗稻瘟霉活性的物质基础.方法利用稻瘟霉筛选体系对红葱的60%(φ)乙醇提取物及其氯仿、正丁醇和水的萃取部分进行活性筛选,并对活性部位的化学成分进行分离鉴定.结果与结论从活性部位中分离得到10个化合物,通过理化性质和波谱数据鉴定它们的结构分别为:异红葱甲素(1)、异红葱乙素(2)、红葱乙素(3)、β-谷甾醇(4)、8-羟基-3,4-二甲氧基-1-甲基-蒽醌-2-羧酸甲酯(5)、4-羟基红葱乙素(6)、hongconin(7)、4,8-二羟基-3-甲氧基-1-甲基-蒽醌-2-羧酸甲酯(8)、dihydro-eleutherinol(9)、1,3,6-三羟基-8-甲基蒽醌(10).其中,9和10为首次从该属植物中分得.化合物2、3、5、6、7、9、10具有较好的诱导稻瘟霉菌丝变形的活性,它们的最小变形浓度(minimummorphologicaldeformationconcentrate,MMDC)分别为30、30、170、130、55、60、150μmol·L-1.用MTT法测定10个化合物的抗肿瘤活性,发现化合物2、3、5、6、7、9有较强的抑制人类红白血病细胞株K562细胞生长的活性,它们的IC50值分别为33、49、266、35、174、154μmol·L-1.     

黄牛木中的异戊烯基酮类化合物

目的 研究黄牛木茎60%乙醇提取物的化学成分.方法 运用多种色谱学方法对黄牛木茎60%乙醇提取物的化学成分进行分离,并根据光谱数据鉴定化合物的结构.结果 从该植物中分离得到5个(口山)酮类化合物,分别鉴定为1,7-二羟基-2-(3-甲基丁-2-烯基)-5"-羟基-6"-甲基-6"-(4-甲基戊-3-烯基)-4",5"-二氢吡喃(2",3":3,4)双苯吡酮(Ⅰ)、5,9-二羟基-8-甲氧基-2,2-二甲基-7-(3-甲基丁-2-烯基)-2H,6H-吡喃一[3,2.b]一双苯吡-6-酮(Ⅱ)、黄牛木(口山)酮A(Ⅲ)、4-(3',7'-二甲基辛-2',6'-二烯基)-1,3,5-三羟基-9H-双苯吡-9-酮(Ⅳ)和黄牛木酮A(Ⅴ).结论 化合物Ⅰ为新化合物,命名为越南黄牛木(口山)酮E(cracochinchinone E).化合物Ⅱ和Ⅳ为首次从黄牛木属植物中分离得到.     

云南石仙桃氯仿萃取物活性部位对入肝癌细胞HepG2的周期抑制作用

目的通过体外实验研究云南石仙桃氯仿萃取物活性部位对人肝癌细胞株HepG2的细胞增殖抑制作用，并进一步研究其作用机理。方法采用噻唑兰（MTT）比色法测定对HepG2细胞生长增殖的影响；采用流式细胞仪技术和蛋白质电泳技术检测对HepG2细胞的细胞周期及相应的细胞周期蛋白表达的影响。结果云南石仙桃氯仿层活性部位通过下调周期蛋白Cyclin B1及其蛋白激酶p34础的表达将Hep G2细胞阻滞在G2／M期。结论云南石仙桃氯仿层活性部位对人肝癌细胞Hep G2的细胞周期具有阻断作用。