人重组γ－干扰素（hrIFN－γ）对人胎盘滋养层hCG分泌和蜕膜蛋白质合成的影响

研究证明人重组γ－干扰素（ｈｒＩＦＮ－γ）对人胎盘滋养层组织绒毛膜促性腺激素（ｈＣＧ）和蜕膜组织蛋白质合成在体外实验中均有抑制作用。结果指出ｈｒＩＦＮ－γ在剂量为２５０Ｕ／ｍｌ培液和２５００Ｕ／ｍｌ培液时明显地抑制滋养层组织ｈＣＧ分泌，与对照组比较Ｐ值分别为０．０５和０．０１，并呈剂量相关反应，同时ｈｒＩＦＮ－γ在剂量范围为１０Ｕ～１０００Ｕ／ｍｌ培液时，明显抑制蜕膜蛋白质合成３Ｈ亮氨酸掺入的ｃｐｍ值，在对照组和实验组之间无论是细胞内或是分泌蛋白都有明显差别（Ｐ＜０．０５），而且抑制作用呈剂量相关反应，ＩＦＮ－γ抗血清能阻断其抑制作用，但ＩＦＮ－α对蜕膜组织蛋白质合成无作用。     

人类早孕蜕膜对B淋巴细胞IgG分泌的影响

目的观察细胞因子对蜕膜细胞介导B淋巴细胞分泌IgG的影响，探讨蜕膜局部免疫微环境的免疫学特点。方法将γ-干扰素（IFN-γ）、白细胞介素．2（IL-2）、白细胞介素-6（IL-6）和表皮生长因子（EGF）加入培养的蜕膜细胞中，用蜕膜细胞的培养上清液刺激B淋巴细胞生长，通过放射免疫法测定作用不同时间后B细胞分泌的IgG的值。结果正常早孕蜕膜细胞的培养上清液可以刺激B淋巴细胞IgG的分泌：经细胞因子作用后的蜕膜细胞培养上清液使B细胞分泌的IgG明显上升，但这种作用与细胞因子的浓度、种类无明显相关性，而与细胞因子作用的时间具有一定关联．表现在第12小时实验组的IgG分泌显著上升（P〈0．05），到24、48h时实验组有逐渐下降趋势。结论在外源性细胞因子的作用下，蜕膜局部体液免疫反应增强，但这种增强可能是蜕膜局部免疫的自身调节作用．以维护妊娠的正常进行。     

人重组γ-干扰素抗生育效应及其机理研究

本研究以新西兰品系实验兔为动物模型,观察人重组－γ干扰素（ｈｒＩＦＮ－γ）的抗生育效应并探讨其作用机理。实验结果：ｈｒＩＦＮ－γ能降低兔胚泡的着床率,对照组的着床率为８０％,ｈｒＩＦＮ－γ２．５、５．０和１０万ＩＵ３组的着床率分别为３０％、２７％和２７％。检测血清中雌二醇（Ｅ２）水平,对照组为１５７．９±３１．８ｐｍｏｌ／Ｌ,ｈｒＩＦＮ－γ各组均有降低趋势,分别为１０７．３±１９．２、１０６．３±２１．７和１０４．９±１９．８０ｐｍｏｌ／Ｌ;血清孕酮（Ｐ）水平,对照组为５０．５９±７．６２ｎｍｏｌ／Ｌ,ｈｒＩＦＮ－γ各组分别为１０．２１±３．１８、６．２３±１．６４和４．８７±０．２６ｎｍｏｌ／Ｌ。在对卵巢和子宫组织的实验病理学的系统观察发现,ｈｒＩＦＮ－γ剂量为１０万ＩＵ时,呈明显的黄体退化、子宫内膜的发育及内膜上皮和腺体的分泌均被抑制。研究表明ｈｒＩＦＮ－γ对孕兔有一定的抗生育效应,其抗孕作用的主要靶器官可能是卵巢,从而影响子宫内环境,使不利于妊娠     

Preliminary Identification of Human Nonserum Oviduct Specific Proteins by Using Electrophoresis and Immunoblotting Analysis

The present paper reported the preliminary results of identification of human nonserum oviduct specific proteins. The ID-SDS-polyacrylamide gel electro-phoresis (PAGE) and 2D SDS-PAFE in conjunction with the immunoblotting assay were used in the present study. The results showed that the nonserum oviduct specific proteins with MW130, 100 and 80 kD existed in human oviduct fluid or oviductal extract. In addition, the antibody against pig oviduct antigens couldmore strongly cross react with human oviduct antigens, mainly recognizing 130,116 and 100 kD proteins from human oviduct. It is suggested that in human oviduct there are some specific antigens possessing some similar epitopes to those in pig oviducts. This result seems to be consistent with predominant cross reactivity existing in antigens of porcine and human zona pellucida.     

The synthetic fragment of rabbit blastocyst peptide＿2 and its physiological significance relevant to

Ｔｈｅｓｙｎｔｈｅｔｉｃｆｒａｇｍｅｎｔｏｆｒａｂｂｉｔｂｌａｓｔｏｃｙｓｔｐｅｐｔｉｄｅ＿２　ａｎｄｉｔｓｐｈｙｓｉｏｌｏｇｉｃａｌｓｉｇｎｉｆｉｃａｎｃｅｒｅｌｅｖａｎｔｔｏｔｈｅ　ｍｅｃｈａｎｉｓｍｏｆｉｍｐｌａｎｔａｔｉｏｎＣａｏＹｏｎｇｑｉ...     

米非司酮对子宫内膜整合素亚单位σ和β3表达的影响

为探讨米非司酮对与子宫内膜接受性建立相关的整合素的影响。对１９例正常生育妇妇进行两个连续月经周期的前瞻性、自身对照研究。应用免疫组织化学方法检测口服米非司酮前后种植窗期子宫内膜整合素亚意境α４（１９例）和β３（９例）、以及孕激素受体（ＰＲ，１９例）表达的情况，应用ＨＳＣＯＲＥ评分作半定量分析，用非参数Ｗｉｌｃｏｘｏｎ检验作统计学处理。结果：种植窗期正常子宫内膜腺体及血管内皮表达整合素亚单位α４和β     

人重组γ—干扰素（hrIFN—γ）对人胎盘滋养层hCG分泌和蜕膜蛋白质合…

研究证明人重组γ－干扰素（ｈｒＩＦＮ－γ）对人胎盘滋养层组织绒毛膜促性腺激素（ｈＣＧ）和蜕膜组织蛋白合成了在体外实验中均有抑制作用，结果指出ｈｒＩＦＮ－γ在剂量为２５０Ｕ／ｍｌ培液和２５００Ｕ／ｍｌ培液时明显地抑制滋养层组织ｈＣＧ分泌，与对照组比较Ｐ值分别为０．０５和０．０１，并呈剂量相关反应，同时ｈｒＩＦＮ－γ在剂量范围为１０Ｕ～１０００Ｕ／ｍｌ培液时，明显抑制蜕膜蛋白质合成＾３Ｈ亮氨酸掺入的ｃ     

The Effect of Human Recombinant Interferon Gamma （hrIFN-γ） on hCG Secretion of Trophoblast and Protein Synthesis of Decidual Tissue in Vitro

In this study the effect of human recombinant interferon gamma (hrIFN-γ) on hCG secretion of human first trimester trophoblast and protein synthesis of decidual tissue was investigated in vitro. The results indicated that hrIFN-γ at the doses of 250 U/ml medium and 2500 U/ml medium decreased hCG secretion of trophoblast obviously (P ＜ 0. 0,5, P＜0. 01 ) in a dose dependent manner. The effect of hrIFN-γ on protein synthesis at the doses of 10 U to 1,000 U/ml medium inhibited the^3 H leucine incorporation obviously. The cpm values between control and experimental groups were significantly different (P ＜0. 05 ) in a dosedependent manner. Furthermore its inhibitory effect is in a dose-dependent manner and was neutralized by IFN-γ antiserum. The IFN-α at the doses used did not find any effect on protein synthesis in decidual tissue.     

Identification and Partial Purification of lnterferons from Human FirstTrimester Trophoblast Tissue

Previous researeh demonstrated the presence of interferons with unusual moleeularweights and amigenicity in virus-infected amniotie membrane and in placental bloodat mid-term and term pregnaney. We reported here the identificution and partial purification of interferons from aeid extract of humen first trimester trophoblast tissue,Immunoreactive interferons, inchuling interferon-x and -β were detected with immunoelectrophoresis and enzyme-linked immunosorbent assays, By preparative is oelectrofocusing, preparative polyacrylamide get elcurophoresis and electroelution,one relatively pure protein with molecular weight of 43 kilodalton was found to have interferon x/β immunoreactivhy,the physiolo gical role(s)of these interferons in the maintenance of pregnancy remains to be defined.