Catquest-8SF量表分析年龄相关性白内障患者的效果

目的：应用Catquest-8SF量表研究PHACO+SIOL植入术患者主观报告的效果,评估Catquest-8SF量表在中国北方白内障人群的适用性及其对白内障手术的敏感性。

方法：前瞻性临床病例随访研究。泰安市中心医院白内障患者术前和术后3mo完成面对面的Catquest-8SF量表填写。

结果：术前共120例白内障患者完成了Catquest-8SF量表。术前Catquest-8SF量表得分与术前双眼加权logMAR视力及年龄呈显著负相关(P<0.05),与文化程度呈正相关(P<0.001),无全身合并症的患者量表得分高于有全身合并症的患者(P<0.05)。102名(85%)患者完成术后3mo量表,单眼手术组和双眼手术组比较,术后视力均显著提高(t=10.404, P<0.001),术后量表得分均明显提高(P<0.001)。两组在量表得分提高值上差异无统计学意义。92.2%的患者对手术效果满意。满意度与量表得分的提高值显著相关(P<0.05)。

结论：中文版Catquest-8SF量表在我国北方ARC人群中适用性好,简短高效,可以作为白内障常规临床评估的一部分。     

Effects of 530 nm monochromatic light on basic fibroblast growth factor and transforming growth factor-β1 expression in Müller cells

AIM: To expose rat retinal Müller cells to 530 nm monochromatic light and investigate the influence of varying light illumination times on basic fibroblast growth factor (bFGF) and transforming growth factor-β1 (TGF-β1) expression.METHODS: Three groups of rat retinal Müller cells cultured in vitro under a 530 nm monochromatic light were divided into 6, 12 and 24h experimental groups , while cells incubated under dark conditions served as the control group. The bFGF and TGF-β1 mRNA expression, protein levels and fluorescence intensity of the Müller cells were analyzed.RESULTS:ThebFGF mRNA expression and protein levels were significantly upregulated in Müller cells in all three experimental groups compared with the control group (P<0.05), while that of TGF-β1 was downregulated (P<0.05). Also, bFGF expression was positively correlated, but TGF-β1 expression was negatively correlated with illumination time. The largest changes for both cytokines were seen in the 24h group. The changes in bFGF and TGF-β1 fluorescence intensity were highest in the 24h group, and significant differences were observed among the experimental groups (P<0.05).CONCLUSION:The expressions ofbFGF and TGF-β1 changed in a time-dependent manner in Müller cells exposed to 530 nm monochromatic light with 250 lx illumination intensity. Müller cells might play a role in the development of myopia by increasing bFGF expression or decreasing TGF-β1 expression. Changes in cytokine expression in retinal Müller cells may affect monochromatic light-induced myopia.     

Successful recovery of COVID-19-associated recurrent diarrhea and gastrointestinal hemorrhage using convalescent plasma

Background:Gastrointestinal symptoms are not rare among coronavirus disease 2019(COVID-19)patients,but there have been no reports regarding convalescent plasma therapy for the recovery of gastrointestinal problems in COVID-19 patients.Case presentation:We present two cases of patients with COVID-19-associated recurrent diarrhea and positive fecal occult blood who successfully recovered after a one-time convalescent plasma administration.Conclusion:When COVID-19 patients develop recurrent or refractory gastrointestinal symptoms and fail to respond to the available treatment,alternative therapy with convalescent plasma administration may be considered.     

新碳青霉烯类抗生素百纳培南的小鼠泌尿道感染药效学研究

目的评价新碳青霉烯类抗生素百纳培南对小鼠大肠埃希菌泌尿道上行性感染的体内疗效。方法通过泌尿道钝针头插管法,向小鼠膀胱内注入0.05 ml浓度为1010cfu/ml的大肠埃希菌9612菌液,建立小鼠泌尿道感染模型。于感染后6、24和30 h皮下给药,感染后48 h处死动物,取肾剖面盖印并研磨计数,对百纳培南进行体内药效学评价,并与对照药美罗培南、厄他培南进行比较。结果对大肠埃希菌9612泌尿道上行性感染小鼠,百纳培南8、2和0.5 mg/kg剂量组小鼠肾组织匀浆菌落计数明显低于对照组(P0.01);8、2、0.5和0.125 mg/kg剂量组肾剖面盖印结果均与对照组有显著差异(P0.01或P0.05)。百纳培南与相同剂量美罗培南、厄他培南组相比,除0.125 mg/kg剂量组肾剖面盖印结果与厄他培南组有显著性差异(P0.01)外,其他剂量组肾组织匀浆菌落计数和肾剖面盖印结果均无显著性差异(P0.05)。结论对于小鼠泌尿道大肠埃希菌9612上行性感染,百纳培南可显著降低小鼠的肾组织菌落计数和肾剖面盖印阳性率,疗效与美罗培南、厄他培南相近。     

The patient-reported outcome of age-related cataract using Catquest-8SF questionnaire

AIM: To assess the patient-reported outcome of phacoemulsification with posterior chamber single focus foldable intraocular lens (SIOL) using the Chinese version of Catquest-8SF questionnaire (Catquest-8SF-CN), evaluate the applicability of Catquest-8SF scale in northern Chinese and its sensitivity to cataract surgery. METHODS: Prospective clinical case follow-up study. The patients were recruited from the Central Hospital of Tai’an and completed the Catquest-8SF questionnaire by face-to-face interviews before and 3mo after surgery at the hospital. RESULTS: A total of 120 cataract patients with median age 67.6y and 52.5% female completed the Catquest-8SF. The Cronbach’s α coefficient of the total scale, daily-activity sub-table and comprehensive-evaluation sub-table were 0.861, 0.853 and 0.748 respectively. There was a high level of consistency between two investigators. The cumulative contribution rate was 66.64% by using the principal component analysis and the maximum variance orthogonal rotation method. The preoperative score of the Catquest-8SF-CN had a significant negative correlation with preoperative binocular mean weighted visual acuity and age (P<0.05), while a significant positive correlation with education level (F=6.094, P<0.001). The patients without systemic comorbidities got higher score than those who with (P<0.05). Three months after surgery, 102 (85%) patients came for follow-up and completed the questionnaire. Significant improvement of visual acuity was observed in both binocular and monocular surgery groups (t=10.404, P<0.001). There was higher improvement in binocular weighted visual acuity of binocular surgery group than in monocular surgery group (t=-20.77, P<0.001). The postoperative score was significantly higher in both groups than before (P<0.001). There was a significant improvement in the total score after cataract surgery. The 94 patients (92.2%) were very satisfied or satisfied with the operation; 92 patients (90.2%) thought the surgery achieved their expectations, and they all felt satisfactory. The degree of satisfaction was significantly correlated with the improvement score of the scale and it was also correlated with their expectation of the surgery. CONCLUSION: The Chinese version of Catquest-8SF questionnaire is suitable for the age-related cataract population in northern China. It is highly responsive to cataract surgery and brief, so it may have the potential to become part of a routine clinical assessment for cataract surgery in China. The patient’s overall satisfaction is high, which illustrates that the medical service we provide matches the patient’s needs. The degree of satisfaction was significantly correlated with the improvement score of the scale and it was also correlated with their expectation of the surgery.     

A new indole alkaloid from the traditional Chinese medicine Chansu

A new indole alkaloid N′-formylserotonin (1), along with five known indole alkaloids N′-methylserotonin (2), 5-hydroxy-1H-indole-3-carbaldehyde (3), N-acetylserotonin (4), 6-hydroxy-1-oxo-3,4-dihydro-β-carboline (5), and bufoserotoin C (6), were isolated from the water extract of traditional Chinese medicine Chansu. Their structures were elucidated on the basis of spectral analyses. The cytotoxicities of 1–6 against human lung adenocarcinoma epithelial cells A549 were tested using the MTT method. Compound 6 exhibited stronger cytotoxic effect than 5-FU, and 1–5 showed no cytotoxic effects. Bufoserotonin C is one of the cytotoxic components in water-soluble extract of Chansu.     

Adult onset type 2 familial hemophagocytic lymphohistiocytosis with PRF1 c.65delC/c.163C>T compound heterozygous mutations: A case report

BACKGROUNDFamilial hemophagocytic lymphohistiocytosis (FHL) is a primary immunodefici-ency disease caused by gene defects. The onset of FHL in adolescents and adults may lead clinicians to ignore or even misdiagnose the disease. To the best of our knowledge, this is the first report to detail the clinical features of type 2 FHL (FHL2) with compound heterozygous perforin (PRF1) defects involving the c.163C>T mutation, in addition to correlation analysis and a literature review.CASE SUMMARYWe report a case of a 27-year-old male patient with FHL2, who was admitted with a persistent fever and pancytopenia. Through next-generation sequencing technology of hemophagocytic lymphohistiocytosis (HLH)-related genes, we found compound heterozygous mutations of PRF1: c.65delC (p.Pro22Argfs*29) (frameshift mutation, paternal) and c.163C>T (p.Arg55Cys) (missense mutation, maternal). Although he did not receive hematopoietic stem cell transplantation, the patient achieved complete remission after receiving HLH-2004 treatment protocol. To date, the patient has stopped taking drugs for 15 mo, is in a stable condition, and is under follow-up observation.CONCLUSIONThe delayed onset of FHL2 may be related to the PRF1 mutation type, pathogenic variation pattern, triggering factors, and the temperature sensitivity of some PRF1 mutations. For individual, the detailed reason for the delay in the onset of FHL warrants further investigation.     

快速认知筛查测验在检测轻度认知损害中的应用研究(英文)

目的轻度认知损害(mild cognitive impairment,MCI)可根据认知表现分为遗忘型MCI(aMCI)和非遗忘型MCI(naMCI)。本研究旨在编制快速认知筛查测验(quick cognitive screening test,QCST)便于快速全面地识别MCI,为进一步研究提供依据。方法符合MCI操作性诊断标准的MCI组121例、符合DSM-Ⅳ有关痴呆诊断标准的阿尔茨海默病(AD)组79例和正常老年人组186例,参与了QCST和标准化全套神经心理测验。参与者教育程度均在5年或以上,年龄55-85岁。QCST项目包括即刻记忆、延迟回忆、命名、动物流畅性、相似性、彩色连线B、画钟、手指结构、数字广度等9个分测验,每个分测验满分10分,总分90分,耗时10-15分钟。结果MCI组、AD组和正常老年人组QCST总分分别为(58.13±8.18)、(44.53±10.54)和(72.92±6.85)分。制定教育程度在5-8年、9-12年、高于13年3个组别的QCST总分的划界分分别为63、65和68分。QCST识别MCI的敏感性为87.6%,其中识别aMCI-s、aMCI-m和naMCI的敏感性分别...     

埋线刺络法治疗强直性脊柱炎临床观察

目的:探讨埋线刺络法治疗强直性脊柱炎(AS)的疗效和安全性.方法:86例AS患者随 机分为埋线刺络组(43例)和药物组(43例),分别予埋线刺络治疗(穴取肾俞、大抒、夹脊等)和口服柳氮磺胺吡啶(SASP)治疗,连续治疗8周,比较治疗前后症状积分、疼痛视觉模拟评分(VAS)、晨僵时间、Schoher试验、枕墙距、胸廓活动度及血沉(ESR)、C反应蛋白(CRP)的变化.结果:埋线刺络组总有效率为83.7%(36/43),优于药物组的70.0%(28/40)(P＜0.05).两组的全部观察指标均较治疗前明显改善(均P＜0.01),且埋线刺络组症状积分、Schober试验和疼痛VAS评分的改善程度明显优于药物组(P＜0.01,P＜0.05),并且埋线刺络组无不良反应.结论:埋线刺络法治疗强直性脊柱炎疗效确切、无不良反应,优于口服柳氮磺胺吡啶.